Video of DNA sequencing technique - (Jul/17/2006 )

I have not done DNA sequencing before, but have to perform it, so i wonder if you know any links or websites that showing us how to perform it (gel preparation, sample loading and detection)?


Thanks alot!

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what machine will you use to perform sequencing?

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It is automated sequencing using a genetic analyzer. I have used BigDye and cycle sequenced the DNA but i have not performed the gel separation and detection parts. i wonder what to do next after i have cycle sequenced the DNA. Would anyone mind telling me how to do it?

For example how you do with:
-sample preparation after cycle sequencing?
-sample loading?
-gel separation?
-detection?


Thank you so much!

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autumn, have you contacted ABI or gone to their website to see if they offer a tutorial?

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