how to compare my DNA sequence with NCBI sequence? - (Jan/14/2008 )
Hi! Dear forumers, I get a PCR product which is 378bp theoretically, and agarose gel confirmed this result
but My sequence result from Invitrogen Shanghai gave me two abi files one is 341bp and another one is 347bp
how do I compare this with NCBI cDNA sequence?
I mean how can I discern point mutation or deletion for example from this result??
First of all you should check quality of your sequences (e.g. if peaks are high and well defined). Do you know what sequence it is? If you do, you can align it against known DNA sequence. If you don't just go to NCBI, Blast, paste your sequence and wait for results.