DNA contanmination in RNA prep. - (Jul/06/2001 )
I'm trying to look for a efficient method to remove the DNA contanmination in my total RNA samples. I'm working with a Gram-positive bacteria E. hirae and I'm trying to use the RNA for quantitative RT-PCR. I've used both the Gaunidium thiocyanate method and the Qiagen's RNeasy kit to extract my RNA. I then treated the RNA with DNase I. However, my no RT control kept giving me positive signal in my PCR.I'll appreciate it very much if someone can give me an advice. Thank you.
You can find a good answer to your question at www.ambion.com/techlib/tb/tb_181.html.when you.good luck.