pmol calculation for DNA - (Jul/20/2006 )

recently i suffered for self-ligated plasmid. so i need to check my CIAP treatment

my question is ...1. how you calculate the amount of 5'end in pmol???
2. I follow Sambrook et al and they wrote 0.1-0.5 U of CIAP i need to use, is it enough for CIAP treatment or Its just theoritical, i need to add more enzyme for successful dephosphorylation??

advacne thanks for your reply

you have the appropriate tool at promega's biomath calculator, including formula.

hi fred many thanks for your reply and the nice link.
but what about my seceond question............is it a theoritical value, do i need to adjust it with my expt?? what do you u do when u dephosphorylate ur vector?