DNA Extraction-pigment contamination - (Apr/14/2007 )

I extracted DNA from plant shoot meristem tissue. However, the DNA that I got is reddish brown in color (anthocyanin contamination?) I used the CTAB method and repeated the wash buffer step several times to get rid of the color...didn't work :-(

Can anyone suggest some way to get rid of the color?

-SonaliM-

Why do you care? Often colored material can be removed with carbon black followed by filtration.

-phage434-

Wouldn't it affect PCR and Southern hybridisation?

-SonaliM-

Probably not. Try it and see.

-phage434-

Well my PCR didn't work today :-((

-SonaliM-

Is there a control reaction which is working? Do you know the primers and annealing temperature are working? Often the problem is too much template DNA. How much are you adding?

-phage434-

QUOTE (SonaliM @ Apr 14 2007, 11:20 PM)

There was already a thread about this, look here

-hobglobin-