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How can I add sequences to DNA using PCR? - (Jun/20/2005 )

I'm trying to add two tails, one on each side of a DNA sequence of interest. My tags are 61 and 66bp long. Does anybody knows where I can find information about how to do this? I'm not getting any product.

Thank you

-medchemgirl-

what is the Tm of your gene specific part of the primer sets?

can you perform a second PCR with just the tails themselves?

Nick

-methylnick-