Protocol Online logo
Top : Forum Archives: : Molecular Biology

How can I add sequences to DNA using PCR? - (Jun/20/2005 )

I'm trying to add two tails, one on each side of a DNA sequence of interest. My tags are 61 and 66bp long. Does anybody knows where I can find information about how to do this? I'm not getting any product.

Thank you


what is the Tm of your gene specific part of the primer sets?

can you perform a second PCR with just the tails themselves?