How can I add sequences to DNA using PCR? - (Jun/20/2005 )
I'm trying to add two tails, one on each side of a DNA sequence of interest. My tags are 61 and 66bp long. Does anybody knows where I can find information about how to do this? I'm not getting any product.
what is the Tm of your gene specific part of the primer sets?
can you perform a second PCR with just the tails themselves?