Largest viral insert? - How much DNA can be packaged? (Apr/17/2004 )
I was wondering how large of an insert I can put into a viral plasmid? I plan to put a 7kb insert into a 5.1kb viral plasmid (pBabe-puro). I'm worried that this large insert won't package correctly.
Does anyone have any experience with this?
I had successful experience of cloning 12kb insert into pGem (3kb). Still think it is not limit.