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What EDTA does in annealing of DNA and vector? - (Oct/05/2007 )

I am using Ligation independent cloning (LIC) for insertion of target DNA into vector (pEMT-11).
From the protocol manual, the next step after mixing T4 polymease digested vector and DNA for annealing, is to add 1ul of EDTA and stand for 5 mins.
I 've been asking why do I add it and how important is it?

Or anyone know what function EDTA is (what for)? Or when do you use it?

-Nwt-X-

what i know about EDTA is Antibacterial

-biojoe-

Apart from being a useful buffer, EDTA is mostly known as a metal chelator. Depending on the concentration (which you didn't give... wink.gif ), I suppose it could also act to stabilise the non-covalently joined insertion prior to transfecting into the cell.

-swanny-