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Small DNA fragments separation - (Oct/22/2008 )

I was wondering if there is any way to separate DNA fragments that differ only 1-10bp.... This is the only way I can think of I could possibly see if second digestion of my vector is successful. (Two restriction enzymes sites are only 4-5 base way to each other...)



A bisacryl/acrylamide gel 7-8%


you mean polyacrylamide gel??? the recipe?? more details please ....