crosslinking of DNA after modification? - (Nov/30/2004 )
I am still trying to optimize my bisulfite modification protocol. I ran it on some size marker DNA in order to compare to the original and quantify how much DNA is lost during the treatment. Not only do I lose a significant amount of the DNA (about 90% less intensity on the gel), there is a bright band "stuck" in the well. Is this really long pieces of DNA that have been crosslinked or ligated during treatment?
Thank you for any help you can give.
-labtechie-
Hi,
did you already try to increase the concentration of the hydroquinone as suggested by Raizis et al (Analytical Biochemistry 226, 161-166, 1995).
Good luck!
-fuechschen-