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Chloroplast DNA isolation - (Mar/22/2002 )

I am interested if anyone has a method to isolate cpDNA that they know works. Currently I am using an isopycnic/CsCL high speed centrifugation method that does not yield much DNA? I am trying to extract samples enriched for cpDNA from Chlamydomonas (green algae).


though I don't have personal experience with your organism, just a thought:
Can you disrupt the algae in such a way that the chloroplast remains mostly intact? If so, you could try separating them by centrifugation from the cellular material.