genomic DNA isolation from hair - (Apr/17/2007 )
hello,
can anybody reccomend me any kit or method to isolate genomic DNA from hair?
I will use the DNA for genotyping using multiplex PCR, so I need a good yield of DNA during the isolation.
I found that some people used Qiagen. But I still want to know the other alternatives
Thank you 
-audrey-
http://www.protocol-online.org/forums/inde...p?showtopic=760
I tried it, very good
CODE
Cut 10- 15 hair roots about 0.5 cm into a 1.5ml eppendorf tube.
Add 50 ul of 200mM NaOH solution.
Boil the tube in a water bath at 94oC for 10 minutes.
Cool at room temperature and add 50 ul of a solution containing 200mM HCL.
+100mM Tris-HCL pH 8.5.
Your DNA is ready for PCR.
Add 50 ul of 200mM NaOH solution.
Boil the tube in a water bath at 94oC for 10 minutes.
Cool at room temperature and add 50 ul of a solution containing 200mM HCL.
+100mM Tris-HCL pH 8.5.
Your DNA is ready for PCR.
-youngvet-
QUOTE (youngvet @ Jun 3 2008, 07:02 AM)
http://www.protocol-online.org/forums/inde...p?showtopic=760
I tried it, very good
I tried it, very good
CODE
Cut 10- 15 hair roots about 0.5 cm into a 1.5ml eppendorf tube.
Add 50 ul of 200mM NaOH solution.
Boil the tube in a water bath at 94oC for 10 minutes.
Cool at room temperature and add 50 ul of a solution containing 200mM HCL.
+100mM Tris-HCL pH 8.5.
Your DNA is ready for PCR.
Add 50 ul of 200mM NaOH solution.
Boil the tube in a water bath at 94oC for 10 minutes.
Cool at room temperature and add 50 ul of a solution containing 200mM HCL.
+100mM Tris-HCL pH 8.5.
Your DNA is ready for PCR.
only with NaOH and HCl?.......how is that possible?
-Curtis-
hi....
im also working on dna extraction from hair sample.....
can sm1 plz tell me the ingredients i shud use in the lysis buffer...and the quantity of lysis buffer to b addaed to the sample?.....thnx in advance.....anxiously waiting for a reply!
-mala-