Chloroform and DNA precipitation - (Jun/06/2005 )
Can anybody tell me what happens if some chlorofrom is still left in the sample when precipitating the DNA with ethanol after phenol/chlorofrom extraction . Please tell me what happens actually!!
Thanks in advance!
actually nothing happens to your DNA (degradation or whatever). Chloroform will just disturb further reactions. Hence i would recommend to precipitate your DNA with a great volume of ethanol absolute and salts and make several washes (5 minimum with a great volume of Etoh absolute. Then you can wash with ethanol 70% and resuspend in ddH2O or water or what you want.
Thanks Fred!! This info has assured me a lot!! I thought maybe the DNA is not able to precipitate properly, or denature in formamide dye after boiling!