chemical DNA denaturation - (Mar/22/2002 )
Can anyone help me find a method for denaturing DNA with chemicals and/or chemical heat? Thus not using a physical heating source... TIA
-trailmark-
Please visit the DNA methylation protocol http://www.protocol-online.net/molbio/DNA/...esis.htm This
Denature the DNA by adding freshly prepared NaOH (3 M) to a final concentration of 0.3 M. Incubate at 42°C for 30 min.
Good luck!
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