chemical DNA denaturation - (Mar/22/2002 )
Can anyone help me find a method for denaturing DNA with chemicals and/or chemical heat? áThus not using a physical heating source... áTIA
Please visit the DNA methylation protocol http://www.protocol-online.net/molbio/DNA/...esis.htm This
Denature the DNA by adding freshly prepared NaOH (3 M) to a final concentration of 0.3 M. Incubate at 42░C for 30 min.
Thisstep is used to danature DNA (separate two strands) by NaOH: