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chemical DNA denaturation - (Mar/22/2002 )

Can anyone help me find a method for denaturing DNA with chemicals and/or chemical heat? Thus not using a physical heating source... TIA

-trailmark-

Please visit the DNA methylation protocol http://www.protocol-online.net/molbio/DNA/...esis.htm

This step is used to danature DNA (separate two strands) by NaOH:
Denature the DNA by adding freshly prepared NaOH (3 M) to a final concentration of 0.3 M. Incubate at 42C for 30 min.

Good luck!

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