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Answer for how to reuse DNA spin columns.. - (Feb/02/2007 )

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Check this link or the file uploaded for details.
Quoting author:" In this publication we have shown that all kinds of commercial nucleic acid extraction columns can be regenerated and reused for several rounds with a simple treatment of exposing the columns to 1 N HCl for 4 hrs.
This treatment will not affect nucleic acid binding capacity of the resin. Importantly, we have also demonstrated that the regeneration process is absolutely free of carry over contamination."
http://www.biotechniques.com/article.asp?id=112327

-Calvin*-

Cool, thanks Calvin*

But, has anybody tried it yet? I wouldn't want to ruin precious samples... unsure.gif

-krümelmonster-

It sounds great. I should email it to my boss. He must love it as our group is not a rich group and boss doesn't like us to waste. It sounds like he is mean. haha. smile.gif
Thank you, Calvin.

-virus_fan-

Thank you Calvin*. smile.gif
It is very useful to reuse DNA spin columns, especially for not so important DNA samples.

I think it would be a good idea to pin this post.

-Minnie Mouse-

Great link! I'll give it a try one of these days and let you know our experience. Nice of Biotechniques to publish a method making molecular biology available to resource-poor and brain-rich countries. biggrin.gif

-Alethea!-

Thanks alot. Now at least there is a journal to back up the procedure!

-timjim-

Thank you! It is very usefull info

-circlepoint-

Interesting article.

My ex-supervisor used to recycle DNA giga prep columns as they were quite expensive.

-scolix-

I am just wondering about the recycling columns. Is it applicable to all types of column? Do you think I can reuse columns used for protein or RNA (I don't think it is a good idea) but I am just curious about it.

-timjim-

I believe it would be applicable to all columns as the basic principle for silicon binding is the same.

-perneseblue-

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