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How to clone large DNA fragment into small vector! - (Mar/09/2002 )

I am doning the research work about subclone. I have a DNA fragment of 30kb which can be digested by EcoRI into 4 fragments. The lenth of them is 5 to 9 kb.I should insert them into a 2.9kb vector respectively. Because the fragments is too long, it is difficult for me to do the subcloning. Could you help me to find a effective protocol to do this work? Thanks a million!


-margaret meng-

did you try a YAC?