Can i introduce strand DNA(not ligate with the plasmid) into E. coli? - (Dec/11/2007 )
detail like in the topic
-DNAchan-
I suppose you can but there's no point in doing it.
Without a plasmid (with a replication origin) that DNA will be lost in a sec.
-Ambrósio-
Can we ask why you want to do this? Are you having trouble cloning?
-swanny-