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Can i introduce strand DNA(not ligate with the plasmid) into E. coli? - (Dec/11/2007 )

detail like in the topic

-DNAchan-

I suppose you can but there's no point in doing it.

Without a plasmid (with a replication origin) that DNA will be lost in a sec.

-Ambrósio-

Can we ask why you want to do this? Are you having trouble cloning?

-swanny-