PCR Genomic DNA - PCR Genomic DNA (Apr/06/2005 )
I'm trying to amplify a promoter by PCR from Genomic DNA of mouse.
I got a good result up to 700bp, then keeping the same Rev primer and changing the forward one to get a product size of 1500 I have faint bands...
I already tried 2 forward primer..both in normal condition and with BD Advanced 2 Taq Pol ( this last pol give me too many bands on the other side )
Thanks for any help
I had same problem before.
What I did is to change annealing temperature (by 1 C at most) and the product band became brighter.