how can I dry down DNA? - (Jul/01/2008 )
Dear all,
I need to transport some DNA samples (dissolved in TE) to my partners for their studies.
They said they want it dried on a 96-well plate and then frozen.
I haven't had experience with that, so I am not sure how it should be processed.
Do I dry off the sample by PCR? But how "dry" does it mean by dried? How would they retrieve the sample if it is dried off?
I guess...I don't quite understand the theory of the method to do it.
Thank you for any clarification! Really appreciate it!
-tictactoe-
You could precipitate DNA with ethanol and dry it out.
-larryking-
QUOTE (tictactoe @ Jul 1 2008, 09:37 AM)
Dear all,
I need to transport some DNA samples (dissolved in TE) to my partners for their studies.
They said they want it dried on a 96-well plate and then frozen.
I haven't had experience with that, so I am not sure how it should be processed.
Do I dry off the sample by PCR? But how "dry" does it mean by dried? How would they retrieve the sample if it is dried off?
I guess...I don't quite understand the theory of the method to do it.
Thank you for any clarification! Really appreciate it!
I need to transport some DNA samples (dissolved in TE) to my partners for their studies.
They said they want it dried on a 96-well plate and then frozen.
I haven't had experience with that, so I am not sure how it should be processed.
Do I dry off the sample by PCR? But how "dry" does it mean by dried? How would they retrieve the sample if it is dried off?
I guess...I don't quite understand the theory of the method to do it.
Thank you for any clarification! Really appreciate it!
For proteins there is a lyophilizer that freezes and dries samples at the same time. Don't know if it will work on DNA.... You could add your liquid DNA sample to the 96 well dish, do salt+ethanol/isopropanol precipitation(look uo any molbio protocol book), wash out the salts with 70% ethanol, then suck out the 70% ethanol so that the DNA remians in the wells. Leave it to air dry or heat it a bit. Then freeze it (in dry ice? Liq. N2?) and send it. Recovering the DNA is easy. They just have to add a small volume of water/TE to the wells and pipette up and down a bit and the DNA will redissolve in the liwuid.
My best suggestion is: Why don't you call them up and ask?
-lotus-
put the DNA in the tube or tray
and then place in a PCR machine
set to 80℃ with the lid open. The
dna should dry in around 10 minutes.
I dunno how long you can keep it
but the faster u send it the better it is...I guess
Just remember not to dry down large
volumes containing reaction inhibitors
like EDTA..～～.. u know why...～～
-desnossjapan-
QUOTE (desnossjapan @ Jul 1 2008, 10:19 PM)
put the DNA in the tube or tray
and then place in a PCR machine
set to 80℃ with the lid open. The
dna should dry in around 10 minutes.
I dunno how long you can keep it
but the faster u send it the better it is...I guess
Just remember not to dry down large
volumes containing reaction inhibitors
like EDTA..～～.. u know why...～～
and then place in a PCR machine
set to 80℃ with the lid open. The
dna should dry in around 10 minutes.
I dunno how long you can keep it
but the faster u send it the better it is...I guess
Just remember not to dry down large
volumes containing reaction inhibitors
like EDTA..～～.. u know why...～～
Thank you for giving me the direction!
Can I ask though how it is possible for to retrieve the DNA?
The PCR heating the samples would only evaporate out the TE??
Sorry..I guess i'm just stuck with the basics so I don't understand the theory behind this~
-tictactoe-
QUOTE (tictactoe @ Jul 3 2008, 03:08 PM)
QUOTE (desnossjapan @ Jul 1 2008, 10:19 PM)
put the DNA in the tube or tray
and then place in a PCR machine
set to 80℃ with the lid open. The
dna should dry in around 10 minutes.
I dunno how long you can keep it
but the faster u send it the better it is...I guess
Just remember not to dry down large
volumes containing reaction inhibitors
like EDTA..～～.. u know why...～～
and then place in a PCR machine
set to 80℃ with the lid open. The
dna should dry in around 10 minutes.
I dunno how long you can keep it
but the faster u send it the better it is...I guess
Just remember not to dry down large
volumes containing reaction inhibitors
like EDTA..～～.. u know why...～～
Thank you for giving me the direction!
Can I ask though how it is possible for to retrieve the DNA?
The PCR heating the samples would only evaporate out the TE??
Sorry..I guess i'm just stuck with the basics so I don't understand the theory behind this~
If the water has been evaporated, all that is left is whatever salts etc were in the original mix. All your collaborators have to do is resuspend in MQW. The DNA will resuspend fairly quickly. If it doesn't, cxover the plate and heat to 65 C for 10 minutes.
-swanny-
QUOTE (desnossjapan @ Jul 1 2008, 06:19 PM)
put the DNA in the tube or tray
and then place in a PCR machine
set to 80℃ with the lid open. The
dna should dry in around 10 minutes.
I dunno how long you can keep it
but the faster u send it the better it is...I guess
Just remember not to dry down large
volumes containing reaction inhibitors
like EDTA..～～.. u know why...～～
and then place in a PCR machine
set to 80℃ with the lid open. The
dna should dry in around 10 minutes.
I dunno how long you can keep it
but the faster u send it the better it is...I guess
Just remember not to dry down large
volumes containing reaction inhibitors
like EDTA..～～.. u know why...～～
this is a very interesting methods, thank you for your sharing. But how long the dry DNA we can storage on which environment?
-mimifather-
QUOTE (mimifather @ Aug 29 2008, 01:54 AM)
this is a very interesting methods, thank you for your sharing. But how long the dry DNA we can storage on which environment?
When you get oligos synthesised for you, they are stable for quite some time at RT. I'd suggest you do an EtOH precipitation in the 96-well plate, cover and seal the top and send it off.
-swanny-