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Is DNA extraction From Tri reagent is good method - (Aug/27/2007 )

I am using zebrafish ovary sample, I tried to extract DNA using the trireagent followed the protocol exactly; at last I got a very big pellet,tried to dissolve it in water, I was not able to dissolve it fully. I spec the dissolved part, I got the ratio of 1.65, concentration was somewhere around 240ng. When I run the agar gel , I got a band v high up adhering to the well ,
My question is it really the DNA?
Why is it so viscous but its concentration is so low??
Why it adhere to the well though I run it on 1.5%, 0.8% and 0.5%?
Is trireagent good enough to extract DNA?
I hv uploaded my 0.5% gel photo here.
your suggestions will help me alot
thx in advance

-vani.khare-

your gel image is not properly attached.

I would recommend that you add a phenol chloroform step (pH8 to their protocol), followed by chloroform only purification.
I would add alsoa RNase step on the DNA sample if you plan to do RTPCR for ex.

-fred_33-

QUOTE (fred_33 @ Aug 27 2007, 12:53 AM)
your gel image is not properly attached.

I would recommend that you add a phenol chloroform step (pH8 to their protocol), followed by chloroform only purification.
I would add alsoa RNase step on the DNA sample if you plan to do RTPCR for ex.


Thx Fred.....I 'll try and see if I can get nice DNA .

-vani.khare-