Recover DNA from gel - How to increase the efficiency of DNA recovery (Aug/05/2002 )
I recovered DNA from gel by Millipore's kit,It's simple.Cut the band from gel and centrifuge for 10 minutes,but the efficiency is disappointed,only 20% or so. Can anyone reccomend me other methods?Thanks in advance
I had initial problems with DNA extraction from gels. But I have no experience with the millipore kit. In our lab we always use the QIa EX kit by Qiagen. THeir recommendations are good enough. But I always get more than 75% when I use TE buffer (pH 8.0, which is very, very critical).
Guess you should give it a shot.
qiagen gel extraction kit works very well for me