How to remove the DNase in a RNA or DNA sample? - (Jul/18/2007 )
How can i remove the DNase in a RNA or DNA sample? Is there any reagent that can help conglomerate DNase and then eliminate it via centrifuge?
If you want to remove DNase and its buffer from RNA sample you can use RNeasy kit produced by Qiagen. It has some centrifuge steps and after all you obtain high amount of RNA samples. I tried and it is ok. This kit also used in RNA isolation. You will apply RNA clean up part. I am not sure about how you can get rid of DNase from DNA samples. Actually I can't understand why you use DNase in your DNA smples.
i prefer phenol chloroform IAA phase separation, as the lenght of RNAs recovered is more wide (mean you can recover the 5S which get through a column). Moreover, the column is not very good i think as the drying is quite long for RNA stability and i don't like it very much.
For you convenience, you may use turbo DNAse from ambion. It's covalently coupled to agarose beads so pelleting and pipetting efficiently remove DNAse. And sample is ok right after this for RT-qPCR.