How can I distinguish exons fromgenomic DNA library??? - (Nov/26/2001 )
I got a partial cDNA sequence by cDNA library screening and 5'race... Because I can not get any more cDNA sequence by 5'race, I am gonna do genomic DNA library sequence.. but the problem is the way to find exon regions from genomic DNA fragment...
Someone help me.. ps) I need cDNA library longer than 1.4kb and I can't buy commercail cDNA library because of being out of money.So cDNA library screening is not possible.
What kind of library? bacterial..cosmid....YAC? and the exons ur looking for...do u know the sequences...or is there sequence published? I was thinking that maybe u could end label a primer and screen it or make pCR primers specific for the exons in question and go that route...email me at email@example.com...we can figure it out