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Help:how much gDNA is needed? - (Mar/21/2005 )

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I want to construct a genomic expression library of a streptococcus spp. But I don't know how much( in ug ) truncated gDNA is needed to construct a representative expression library for immunoscreen. Who can help me?
Thanks a lot!


dear flyinghorse,

It really depent on what vector are you using. for me, I use 100ug before and it work.

Good luck