DNA migration problem - Genomic DNA (Aug/07/2008 )
Last day after I prepared genomic dna , I set up pcr with previously used primerz and I found there was no amplification but there was proper band of g-DNA just below the wells ...do you think excess DNA will inhibit PCR? will remaining phenol in G-DNA prep inhibit PCR?
does remaining phenol in G-DNA prep after PCI also inhibit DNA solubility even after EtOh step?
(I found the DNA to be a bit insoluble after drying)
can G-DNA (excess) trap PCR products and inhibit migration in Gel?
what was the quantity of the reagents and concentration, Tm of the primers, the program of thermocycler and if possible attach a photo of the gel.