Problem for DNA extraction (Trizol) - Problem for DNA extraction (Trizol) (Dec/10/2007 )
Problem for DNA extraction (Trizol)
I gonna to extract DNA from the Trizol fraction (with DNA) (frozen for a month after removing the RNA fraction). The problem is after adding 100% ethanol, the trizol showed high tubidlity, and clody was spin down after the centrifugation.
What cause the tubidity? and how to solve this problem if i need to continute to purify the genoma DNA from this sample.
Thank a lot
the turbidity could be the result of co-extractionof compounds similar to DNA/RNA or due to fats in suspension. Keep on centrifuging the solution until a pellet forms.
Thank you very much