Problem amplifying 8kb bacterial genomic DNA - (Jul/31/2006 )

Hey guys. I´m trying to amplificate a 8kbp fragment for a bacterial genomic DNA and dosen´t function. I was probing Elongase, Taq GO and some mix of enzymes and nothing. The primers are ok but the DNA its a little complicate to extract but function with more little amplificates (2kbp).
Well, some sugestions please?
Thanks.

---

What is the GC content of the organism? Can you test the primers by using them to amplify shorter fragments?

---

Hi, thanks for your request, the GC contet is 58,7. sadly I can´t probe the primers with small fragments. What you can suggest me?
Thanks

---