Attaching DNA bases - Creating primers and plasmids? (Aug/25/2008 )

Hey people,

Could anyone explain or provide a link on how PCR primers are created? I mean how are the bases joined? And from that principle, can one go about creating circular dsDNA (e.g. a plasmid)?

Thanks and have a great day.

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Have you tried googling oligonucleotide synthesis?

Next, consider the cost per base of custom synthesis, and the length of a plasmid, and do the maths. Even if you make each oligo set at 100 nt (which will give you ~10% full length protduct), you're pushing it.

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the efficiency of coupling each new base varies from 98.5 to 99.5. At 100bp you fill find only 60-20% percent (leaning closer to 40% according to invitrogen) of the molecules are correctly synthesized.

Having said that there have been initiative to build plasmids directly from these 100bp primers, with the purification, ligation, amplification steps all on a single chip.

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Thank you for the info swanny and perneseblue. Have a good day.

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