Protocol Online logo
Top : Forum Archives: : Molecular Biology

DNA extraction - Is 2years ago extracted DNA still usable (Aug/10/2004 )

Hi,
Could somebody help me? I have DNA that have extracted two years ago from formalin-fixed and paraffin-embedded tissues. It has been kept at 4 ¢ªC . Can I use it to methylation analysis, is DNA too fragmented and inappropriate for analysis, or should I do the extraction again? Some of the samples are quite old (years between 1986-1996).

-mstefani-

In our lab we successfully use fungus DNA (usually in simple procedures as PCR), which was extracted even 10 years ago.
So you definitely might try
Good luck smile.gif

youlia

-Youlia-

there should be no problem. some of my colleagues keep their DNA, primers all at 4C.

-mario2004-