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DNA extraction - Is 2years ago extracted DNA still usable (Aug/10/2004 )

Could somebody help me? I have DNA that have extracted two years ago from formalin-fixed and paraffin-embedded tissues. It has been kept at 4 ¢ªC . Can I use it to methylation analysis, is DNA too fragmented and inappropriate for analysis, or should I do the extraction again? Some of the samples are quite old (years between 1986-1996).


In our lab we successfully use fungus DNA (usually in simple procedures as PCR), which was extracted even 10 years ago.
So you definitely might try
Good luck smile.gif



there should be no problem. some of my colleagues keep their DNA, primers all at 4C.