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PCR Related Discussions
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PCR product appear two close band in my gel! - (reply: 3)
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Multiplex pcr trouble - (reply: 4)
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need help...how to compare mouse vs human sequences for primer design - (reply: 2)
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RT-PCR for small RNAs - (reply: 1)
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DNA PAGE - suitability of PCR buffer (reply: 1)
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pcr amplification - right amplification,right sequence but still in dilemma (reply: 4)
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Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
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Tm of PCR primers: one 68, the other 58. Impossible to succeed? - (reply: 3)
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PCR product dimer - clean it up (reply: 3)
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Primer design for PCR following ChIP - (reply: 3)
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PCR Inhibitor - Where/What are you? (reply: 9)
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Primer dimers!? HELP - (reply: 8)
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MSP primer does not work - (reply: 5)
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How to increase the product amount in BSP? - weak amplification in BSP (reply: 3)
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general PCR question - (reply: 15)
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What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
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PCR product stuck in agaros wells - (reply: 8)
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how 2 design rev.transPCR primer? - need functioning protocol.... (reply: 1)
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methylnick.please help me with MSP primer designing! - (reply: 3)
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PCR efficiencies for analysis of relative real time data - (reply: 1)
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End of PCR products destroyed - (reply: 2)
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Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
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Inverse PCR ligation - (reply: 1)
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Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
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deisgning primers with restriction site - (reply: 7)
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RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
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cDNA primer help - (reply: 1)
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Background amplification - (reply: 1)
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Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
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Primer design program for Methylight - (reply: 3)
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repeting PCR failing the reaction - (reply: 2)
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mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
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PCR efficiency? - (reply: 3)
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PCR fragment ligation - ligation of 3 pcr frangments (reply: 1)
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What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
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Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
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PCR false positive reasons - (reply: 3)
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PCR cycle - (reply: 1)
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RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
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Why am I only getting smears for my genomic DNA long-distance PCR products? - It worked great with sheep BACs but failed miserably for genomic DNA (reply: 4)
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PCR amplification - (reply: 2)
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primer sequence - reverse primer sequence (reply: 3)
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RT-PCR GAPDH Variability - (reply: 1)
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PCR Optimization Question - Non-specific smearing around target level? (reply: 6)
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PCR troubleshooting - (reply: 10)
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pcr question - (reply: 5)
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PCR bands - why not straight ?&%#@&& - (reply: 3)
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how to optimize rt-pcr for gene expression analysis - (reply: 3)
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PCR negative control contaminated and not the other wells - (reply: 4)
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RNA extraction for real time PCR from specific brain region - (reply: 1)
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PCR Problem - 300bp skip in the middle (reply: 9)
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Designing Primers - (reply: 1)
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Immunostaining - Amplification of signal - (reply: 1)
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rolling circle amplification - (reply: 1)
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Normal PCR for looking expression - (reply: 1)
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internal control primers for plants - (reply: 14)
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Where do you get your PCR primers from? - (reply: 3)
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genomic DNA contamination in RT-PCR - (reply: 7)
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how much cDNA do you use for RT-PCR? - (reply: 2)
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PCR WOES...... - STRATEGY DOUBT... (reply: 3)
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Strange amplification pattern in DNA real time PCR - (reply: 4)
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Could have more DNA concentration inhibition effect on PCR? - (reply: 3)
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BLAST Help , Designing primers... - (reply: 5)
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pyrophosphate generation in PCR - (reply: 2)
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housekeeping gene in klebsiella pneumoniae for RT PCR - (reply: 2)
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RT PCR of viral RNA segment - (reply: 1)
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Primer design and degeneracy - Are synthesized sets identical? (reply: 2)
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panomics promoter methylation PCR kit - results not what i expected (reply: 1)
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primers amplify additional band (!) - (reply: 1)
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resuspended primers in water - is this a problem?! (reply: 5)
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Vent polymerase vs Klenow - (reply: 1)
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Designing the primers for qRT PCR - Query - (reply: 14)
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primers sequence - (reply: 2)
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Chip Troubles - Help: Strong PCR signal from negative control region (reply: 7)
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primer designing - (reply: 1)
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wierd rt-pcr - (reply: 1)
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genomic DNA PCR - is it the same as a cDNA PCR (reply: 9)
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spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
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Unspecific PCR bands - Why that happens? (reply: 5)
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Primer doubts - (reply: 2)
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PCR cloning - (reply: 5)
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Fast vs Standard PCR - (reply: 1)
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RT-PCR to amplify ORFs - (reply: 3)
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Transcript variation - measuring by RT-PCR - (reply: 3)
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Problem with promoter PCR and cloning - (reply: 3)
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PCR components/DNA sample optimal amounts - ul of DNA and each component for a good reaction. (reply: 10)
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please help me to be sure from design primer have it - (reply: 3)
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heparin in DNA - no working PCR (reply: 4)
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when to set up a RT-PCR for mRNA expression? - detect transcription of transgene (reply: 1)
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When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
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Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
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resuspend Primers in TE or in water? - (reply: 7)
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ssr cross species amplification - (reply: 2)
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Can we measure gene expression with standard PCR? - (reply: 1)
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Abgene PCR master mix?-MgCl concentration - (reply: 1)
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Sybr Green real time pcr limit of detection - (reply: 1)
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Taq polymerase expressing E. coli requested - (reply: 2)
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BLAST search of primer sequence - (reply: 3)
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Best house keeping gene for RT-PCR - (reply: 6)
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RT-PCR inconsistant results - (reply: 1)
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Oligo dT primer - oligo dT primer with non-T priming 3´ or with 5´ (reply: 3)
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PRC reagents and equipments - Is a PCR machine robust? (reply: 2)
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Large smear in PCR - (reply: 2)
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developing real time PCR - (reply: 5)
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false positives in PCR due to environmental contamination? - (reply: 7)
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Primer design - How to - am a novice ? (reply: 6)
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Primer optimization (with probes) - Urges... (reply: 2)
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arms PCR problem - (reply: 2)
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storing pcr samples - (reply: 19)
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RT-PCR reagents KIT 1 or 2-step? - (reply: 3)
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Extraction of PCR product from gel - (reply: 4)
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Choice of one-step or two-steps RT-PCR - (reply: 3)
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Problems with Bisulfite PCR - (reply: 13)
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Choosing Primer: random or Olig-dt ? - (reply: 2)
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how to synthesize the pcr primers - (reply: 3)
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Washing the spin columns from PCR purekit - (reply: 1)
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Trouble inserting PCR derived insert into vector - Trouble inserting PCR derived insert into vector (reply: 2)
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RT-PCR primer design and in silico PCR - (reply: 4)
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Checking mRNA expression by RT-PCR - (reply: 2)
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primer design - over expression (reply: 1)
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Bisulfite PCR Question - (reply: 2)
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quadraplex RT-PCR error - (reply: 1)
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cloning of a fluorecent PCR product - (reply: 1)
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NOT SyBrGreenPCR amplification in positive control-Why? - (reply: 1)
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how to improve PCR yield? - (reply: 5)
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TEST: embed video - PCR song - (reply: 4)
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PCR to sequencing problems - (reply: 5)
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EXOSAP-IT degrades my PCR products - (reply: 3)
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Problems with amplification - (reply: 7)
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PCR master mix preparation - (reply: 11)
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real time pcr newbie - (reply: 3)
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PCR: Big Primers = Big Problems (for me) - I'm really not sure what's going on in those poor little tubes... (reply: 2)
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mutations in primer sequence - (reply: 2)
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Amount of DNA to run on gel - Be it from PCR or from DNA extraction steps (reply: 4)
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Amplification Effeciency - solve the problem with E value (reply: 2)
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PCR Inhibition by DNA? - (reply: 5)
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RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
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Total RNA isolation for RT-PCR - (reply: 3)
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Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
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primer conditions for expression work - (reply: 5)
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Failed RT-PCR amplification of 3-&5''-ligated small RNAs - (reply: 5)
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Question about primer design targeting GC-rich region - (reply: 2)
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how to optimize primer and cDNA concentration for real-time - (reply: 2)
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Help -designing primers for real time RT-PCR - (reply: 1)
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getting long pcr from cDNA - (reply: 4)
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PCR from cDNA library - (reply: 1)
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clear culture, but PCR positive - (reply: 4)
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Bad primer efficiency during multiplex qPCR - (reply: 1)
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phophorylating PCR product after CIP - (reply: 2)
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pcr template amount - (reply: 2)
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PCR with paraffin-embedded tissue DNA samples - I have had problems to amplify DNA samples from paraffin-embedded tiss (reply: 1)
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Problems with amplification curve - (reply: 5)
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different transcription from 5' UTR than 3' UTR primers - (reply: 8)
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recommended primers for caspase 8 & 9 - human (reply: 4)
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Optimising bisulphite PCR primers - (reply: 4)
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Strange large bands on my PCR gel, has anyone seen this before? - PCR (reply: 3)
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help with RT-PCR - DNase usage - (reply: 2)
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PAGE with Realtime PCR product (Sybr Green) - Post-staining with ethidium bromide? (reply: 4)
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PCR inhibition in FFPE DNA - (reply: 1)
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Determine specific primers and transgene copy number by PCR - (reply: 1)
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PCR with Betaine, DMSO, BSA, DTT - (reply: 5)
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A couple of (RT-PCR) cowboy questions - I feel the need, the need for speed (reply: 2)
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PCR on this gene - products always present? - (reply: 9)
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PCR cycle for 80 bases - (reply: 2)
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High concentration of antibiotics in amplification of plasmids - concerns about mutation of inserts in plasmid (reply: 1)
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my negative PCR control is still positive ! - (reply: 32)
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Getting primer dimers in RT-PCR on and off? - (reply: 2)
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PCR reaction - quick question! - (reply: 2)
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conc of cDNA to be used - PCR to test primers (reply: 5)
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optimum conditions for long pcr - (reply: 1)
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DIG pcr labeling and northern blot - (reply: 2)
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Weekly Decontamination Routine? - To prevent DNA contamination in a PCR lab (reply: 1)
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PCR on template with trinucleotide repeats - PCR tips for complexe DNA template (reply: 4)
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1:10 serial dilution in rt-pcr with slope of -0.58 ... - (reply: 1)
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Lengthy primers - (reply: 11)
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positive colony PCR, but no insert!? - (reply: 5)
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Steps in PCR - (reply: 1)
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ORF region amplification problem - (reply: 2)
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need help on microRNA real-time PCR - (reply: 6)
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PCR Help! - Primers-quality checking software (reply: 3)
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design primers - (reply: 3)
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DNA polymerase that produces blunt end and doesnt smear! - (reply: 2)
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PCR on Genomic DNA from cell lysate - (reply: 5)
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Primers distinguishing splicing alternatives - (reply: 1)
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Problem with bands of PCR products - (reply: 2)
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about PCR efficiency - (reply: 2)
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TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
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primer design from mRNA - (reply: 12)
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How do you prevent PCR evaporation? - (reply: 9)
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PCR contamination - (reply: 1)
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How can I create a T vector for PCR product cloning - (reply: 2)
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PCR product won't come down from well - (reply: 3)
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RE of PCR Fragment - (reply: 2)
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Starting with semi quantitative RT-PCR and a little bit lost.. - (reply: 4)
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Storing primers...together? - (reply: 3)
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different length for primers amplifying - (reply: 2)
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cytosolic PCR control - (reply: 1)
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Primer concentration is quantitative Real-time PCR - (reply: 1)
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primer dimer annealing temperature - (reply: 2)
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What is the function of acetate solution in DNA extraction for PCR used - (reply: 6)
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choosing real-time PCR machine - (reply: 13)
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Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
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some primer dilution calculations - (reply: 7)
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PCR - reduced binding efficiency with lower Tm? - (reply: 2)
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Purified PCR product - (reply: 2)
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Housekeeping genes for RT-PCR in soybean - (reply: 6)
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cDNA quantification for PCR - (reply: 5)
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no PCR product - (reply: 14)
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Can do reverse transcription by gene-specific primers? - (reply: 3)
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Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
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plz save my PCR product ! - (reply: 4)
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primer desighning with RE sites - (reply: 5)
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Primer design (long transcripts) - (reply: 2)
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Primers for Real time PCR - Really need some advice (reply: 1)
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RNA amplification - (reply: 3)
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PCR product of unlinearized plasmid - (reply: 1)
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help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
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do you put two pairs primers in one tube when you do site mutations? - (reply: 2)
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Will TOPO TA cloning work on "old" purified PCR product? - (reply: 4)
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Can PCR on same sample give different results when repeated? - (reply: 2)
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RT PCR for methylation analysis - (reply: 3)
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RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
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chip assay primers - chip assay primers (reply: 2)
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suitable length DNA for PCR? - (reply: 2)
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how to add bases to the primer restriction sites - (reply: 2)
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Problems with making mix of PCR reagents ? - (reply: 4)
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primer design - (reply: 3)
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SYBR green PCR - (reply: 4)
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PCR problem, no amplicon, tried everything I could think of.. - (reply: 6)
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How to ressuspend primers for Sybr? - (reply: 3)
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simple primer calculation - (reply: 5)
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What's wrong with my pcr? - high temperture with low specificity (reply: 6)
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RT-PCR Vs qRT-PCR help please! - (reply: 4)
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300 bp more after PCR purification - please help - (reply: 3)
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primer/probe mix for standard generation? - (reply: 2)
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Fast way to get plasmid for PCR - (reply: 2)
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PCR efficiency for qRT-PCR analysis - PCR efficiency for qRT-PCR analysis (reply: 4)
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PCR failure. GC problem? - (reply: 7)
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Help. Trying to sequence my plasmids, but the primers refuse to prime! - (reply: 1)
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Degnerate PCR and Sequencing Reactions - (reply: 4)
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Forward n reversre primer Tm difference is 10 degree - (reply: 1)
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Smearing in PCR - (reply: 4)
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Why using heated-top or add mineral oil in PCR - (reply: 2)
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primer design (downloadable) programs - not online tools (reply: 4)
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BLOOD storage and PCR problems! HELP - (reply: 1)
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PCR water contamination - (reply: 5)
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figure out the size of amplicon using known primer - (reply: 3)
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Primer concentration... - (reply: 5)
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How to amplify PCR product - wonder why using linear fragment as template, PCR result was always sm (reply: 1)
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Long PCR primers for epitope tagging - where to order? (reply: 3)
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Extremely Low Yield PCR with restriction site addition - Assistance requested for beginning molecular biologist on PCR techniqu (reply: 3)
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Problems amplifying unstable construct in E.coli - Tips needed (reply: 2)
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RT-PCR consistency - (reply: 2)
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dried out pcr product - (reply: 1)
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Please see the PCR electropherogram - What's wrong with my pcr? (reply: 8)
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PCR inhibitors - help ??!! (reply: 8)
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QPCR primer Pairs - (reply: 1)
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Random Amplification + TA cloning = HATE - (reply: 2)
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Quikchange PCR dNTP mix? - (reply: 8)
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Inconsistent bisulfite PCR - (reply: 8)
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ISH vs. Real Time PCR - correlation between CT-values and quantity - (reply: 2)
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no PCR product! any advice? - (reply: 14)
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New to rt pcr real time - guidelines and advice (reply: 3)
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overlap extension pcr - (reply: 1)
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Quikchange mutagenesis primer and PCR design - (reply: 3)
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PCR inhibitors in soil DNA - (reply: 6)
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How to know tissue specific gene expression by real time PCR - (reply: 3)
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Statistics: real-time RT-PCR - (reply: 4)
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PCR of AT-rich DNA - (reply: 3)
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Primer conc - (reply: 6)
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Non-specific Real time PCR signals - (reply: 1)
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Does anyone know how to design LUX primers - (reply: 2)
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Problems on overlap extenstion PCR - (reply: 2)
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Cox-2 primer for PCR - method how to search good cDNA in Pubmed (reply: 1)
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ChIP PCR problems - I can't get rid of the smears! - (reply: 6)
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PCR primers for CRISPR - primer3 isn't helpful in this case (reply: 1)
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QPCR versus regular PCR for Chip - (reply: 2)
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Is it necessary to measure the amount of DNA before PCR? - (reply: 3)
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miRNA real time PCR - miRNA real time PCR (reply: 2)
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DNA amplification for chip-on-chip - WGA or LM-PCR or others? (reply: 12)
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PCR primer design, real time versus old school - (reply: 2)
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Assembly PCR problem - (reply: 3)
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Amplifying cultures - quick question - (reply: 3)
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I have a problem with the M13 tailed primer method - M13 tailed primers-SSR (plants) (reply: 1)
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PCR Tubes - what kind of lid ? (reply: 6)
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What in ChIP samples inhibit PCR? - (reply: 2)
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Storage of a plate for real time PCR - Ever stored your already prepared plate for real time PCR? (reply: 7)
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specific PCR polymerase - (reply: 3)
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help with site-directed mutagenesis - What polymerase and cells to use? (reply: 3)
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MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN - (reply: 1)
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cloning 708bp PCR prodyct into pET101 vector (TOPO) - (reply: 2)
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Primer3 results - (reply: 2)
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Problems with several unspecific bands after PCR - (reply: 2)
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Colony PCr - (reply: 4)
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Cloning PCR product blunt + restriction site - (reply: 2)
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cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2)
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real time pcr with RNA co-precipitated with glycogen - (reply: 3)
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transfect from full plasmid and transfect from PCR product - (reply: 5)
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PCR problem..... - (reply: 4)
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PCR primers with overhangs - (reply: 2)
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How to reduce the dimers - (reply: 3)
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magnesium concentration in pcr - (reply: 4)
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Primer designing - (reply: 1)
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What is your favorite tool for primer design ? - (reply: 2)
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Primer express? - Do you use it? (reply: 2)
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The best reverse transcriptase for RT PCR - (reply: 5)
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Ooops, forgot to add Mg to PCR! - But samples amplified! Can I use them? (reply: 3)
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Genotyping - Primers for PCR genotyping (reply: 5)
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Enzyme sites on Primer - (reply: 3)
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PCR troubleshooting - (reply: 1)
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gene-specific Primer for qRT-PCR - (reply: 2)
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how to explain this strange amplification chart? - the Ct value comes to be very low because of the unusual amplification (reply: 1)
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PCR Troubleshooting - (reply: 2)
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No signal in Real-time but in normal PCR?! - (reply: 2)
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What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb? (reply: 6)
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deletion PCR? - I need help (reply: 1)
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need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
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PCR troubles - PCR works one day but not the next (reply: 10)
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PCR from cell culture - to detect the presence of adenovirus (reply: 2)
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Wrong PCR product after Bisulfite Treatment-reason? - (reply: 1)
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PCR smear with CHIP - (reply: 2)
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Smear in PCR after ChIP - (reply: 2)
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What happens if different templates dilution in Real-time PCR reaction - (reply: 1)
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PCR amplification of region with many ATT repeats - (reply: 1)
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PCR primers artifact - (reply: 2)
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weak RT-PCR product - (reply: 2)
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How many cycles in PCR would you recommend for ChIP - (reply: 3)
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real time PCR template - template concentration (reply: 3)
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primer design for site-directed mutagenesis - (reply: 3)
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what affects primer specificity? - (reply: 2)
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modification of PCR buffers - When and how (reply: 1)
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how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
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Dealing with VERY Mg sensitive PCR reactions - (reply: 4)
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Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
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Can I use different amount of cDNA template to run real time PCR? - when the amount of templates are different,Can I use reference gene to (reply: 2)
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Human Epigenome Project primers? - (reply: 2)
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Degenerate Primers - Help with Degenerate Primers (reply: 1)
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PCR screening of colonies - (reply: 3)
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Colony PCR running as smears - (reply: 1)
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PCR: Can you get amplification if only one primer anneals? - Same size pcr product using 2 different primer sets (reply: 8)
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RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
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PCR cloning: neg self-ligation control, many colonies but no insert - (reply: 3)
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PCR help! - urgent (reply: 5)
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ready to use primers for BSP - (reply: 4)
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Troubles in PCR the converted DNA in promoter region - (reply: 6)
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Are there any special rules for primer design? - (reply: 5)
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ELISA in a real-time PCR plate - possible? (reply: 3)
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GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
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Unnatural smear in PCR product - (reply: 7)
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ChIP PCR help, maybe is the problem of SDS. - (reply: 4)
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Colony PCR - (reply: 8)
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how to check normal distribution of data - real-time PCR data (reply: 2)
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Insert PCR - (reply: 1)
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mutant PCR - (reply: 4)
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DNA purification technique - After a PCR (reply: 8)
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primer design for Syber Green - (reply: 1)
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DGGE - PCR and gel conditions - (reply: 2)
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PCR machine choice: - (reply: 14)
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PCR OF HIV genome - (reply: 1)
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Cloning a 60 bp pcr product and Mixing Pfx and Taq polymerases - (reply: 4)
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optimizing PCR for low Tm primers - (reply: 1)
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Minimal nucleotide for Taq polymerase binding - (reply: 1)
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primers TM - (reply: 8)
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pcr product degradation - (reply: 1)
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Colony PCR - (reply: 11)
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primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
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designing a primer - (reply: 5)
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using primers programms - (reply: 9)
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qPCR housekeepers - primers or probes? - (reply: 1)
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pcr for gene fusion - (reply: 3)
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basic question regarding PCR - (reply: 7)
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freaky primers - (reply: 5)
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How do you design primers of PCR-STR used in Forensic Medicine - Urgent!!!! (reply: 2)
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what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
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How to get rid of primer dimers - (reply: 2)
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problems with PCR primers - (reply: 15)
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one nucleotide in the primer is wrong - (reply: 2)
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How PCR product becomes gelly? - (reply: 1)
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Flag Tagging a PCR product - (reply: 2)
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genomic DNA extraction - for PCR (reply: 1)
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PCR thermocycler (Eppendorf) - noise problem (reply: 1)
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real time pcr in a day - (reply: 5)
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PCR efficiency - (reply: 2)
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Triton X in PCR - (reply: 7)
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real time PCR using two sets of primers with different melt curves - (reply: 4)
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AmpliTaq Gold - high fidelity polymerase? - (reply: 2)
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Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
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real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
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Introduce restriction sites with PCR primers - (reply: 2)
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How much DNA in PCR? - (reply: 6)
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Free FastStart Universal Real-Time PCR Master Mixes from Roche - (reply: 7)
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primer flaw I'm missing? - (reply: 7)
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Blunt end cloning PCR product - (reply: 6)
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PCR contamination that comes and goes! - (reply: 5)
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How long we can keep Diluted Primer? - (reply: 5)
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Real-time PCR....Making sense of it all? - (reply: 3)
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PCR primer resuspend method? - (reply: 9)
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Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
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RT PCR produces nice crisp band but of wrong length :-) - (reply: 2)
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Help needed. PCR band problem - (reply: 4)
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2/3Kb PCR fragment - 16S/23S (reply: 3)
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Where do dNTP come from? - Chemical or Biological? (reply: 12)
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polimorph markers in inbred mouse strains? - microsatellites or any PCR based suggestins are wellcome! (reply: 1)
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RT-PCR gene specific amplification problem - (reply: 1)
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information regarding PCR using very long primers - (reply: 4)
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Non repeatability of RT-PCR with same cDNA - (reply: 3)
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MSP primer design - understanding the basics - (reply: 2)
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Bisulfite: PCR condition or primer problem? - (reply: 13)
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I really need an answer on PCR (16s rRNA), PLS PLS PLS! - (reply: 2)
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storage of primers - (reply: 3)
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Problem with pcr, help required - PCR product (reply: 12)
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Curious - different primer synthesis? - (reply: 4)
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Windows in PCR/Clean rooms..... - (reply: 8)
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help a student with a PCR process question? - (reply: 6)
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too many hits on Blast for primers / microsatellite - (reply: 4)
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sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
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how to get DNA sequences starting from the primer - (reply: 4)
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How to remove a few bases from my cloned insert using PCR - (reply: 3)
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PCR help - (reply: 1)
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PCR amplification using universal 16s rDNA - it's necessary to do it (reply: 3)
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LM-PCR amplification - (reply: 1)
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RT-PCR NTC- substitute DNA with water? - or just end up with a smaller rxn volume? (reply: 1)
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PCR protocols - for some antibiotic resistant strains (reply: 2)
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How to quantitate the pcr product from gel picturess - (reply: 4)
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primers for promoter amplification - (reply: 1)
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RT-PCR primer designing - (reply: 6)
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Scaling up PCR reactions - (reply: 4)
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Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
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cloning through PCR - (reply: 3)
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Homemade TaqMan PCR Mastermix? - (reply: 1)
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My primers don't work with Chip - (reply: 1)
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qPCR using random primers - (reply: 4)
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designing primers for cloning - designing primers for cloning (reply: 1)
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no bands in PCR reaction analysis - does centrifuge time matter? (reply: 2)
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PCR optimization - (reply: 5)
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1. ABI Power SYBR® Green primer efficiency? - (reply: 1)
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Polymerase Activity Assay - (reply: 1)
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About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
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PCR inespecific band only in control - why only in the control? (reply: 2)
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Identification by 16S rDNA - using short primer (reply: 2)
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Degenerate Primers - (reply: 12)
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What safety and risk assessments should i be aware of in doing PCR and gel elect - (reply: 2)
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restriction enzymes primers - (reply: 5)
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Fusion PCR - Help ragrding Fusion PCR (reply: 5)
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Roche produce Blunt or dA tail PCR product? - (reply: 3)
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Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
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Ever heard not of Prime Dimers but Primer Multimers? - (reply: 1)
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Isolation of the upstream region of a gene by PCR: a new tecnique? - (reply: 4)
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make PCR template blunt ended - (reply: 2)
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AT rich PCR problems - anyone experienced with tetramethylammonium chlroide? (reply: 3)
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how would you detect a point mutation in the genome? PCR? - (reply: 3)
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KOD HiFi DNA Polymerase - (reply: 3)
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Need to confirm PCR primers are bad - (reply: 5)
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PCR - (reply: 1)
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Expectations for presentation of pcr data - (reply: 1)
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False negative PCR result - (reply: 5)
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help with nested PCR for splice variant detection - nested PCR (reply: 3)
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Ligation of half blunt PCR fragments - (reply: 4)
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first time doing quantitative real time PCR - (reply: 1)
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Help! Primer Dimers… - (reply: 3)
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RT-PCR without RNA isolation - (reply: 2)
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Very low PCR primer concentrations? - (reply: 4)
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Inconsistent PCR results - (reply: 3)
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Multiplexing first round PCR in BSP - Any experience? (reply: 16)
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PCR detectable units - PDU (reply: 2)
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Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
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no results in RACE PCR - (reply: 5)
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Very resistand band in PCR - (reply: 7)
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Knockdown detected by western but not RT-PCR - (reply: 3)
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PCR Amplification from clones - (reply: 7)
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Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
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PCR cloning problem - (reply: 6)
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validation of micrarray data by realtime pcr - (reply: 1)
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minimum amount of rna in RT-PCR - (reply: 1)
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Trying to lyse cells to get PCR template - Need something simpler than a miniprep (reply: 10)
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PCR Primer again - (reply: 1)
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RAPD PCR - (reply: 1)
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Polymerase Choice Troubles - my primer melting temp is so low (~30 C) (reply: 4)
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multiplex RT-PCR - (reply: 6)
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cycling parameters for PCR - (reply: 4)
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primer Tm - (reply: 10)
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why no insert but can PCR insert out? - (reply: 5)
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smear in degenerate PCR - (reply: 3)
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Single Codon Deletion - SOE PCR - (reply: 3)
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oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
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PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
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PCR for checking transfection - (reply: 6)
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digoxygenin-labelled primer - (reply: 3)
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How to import GeBank files into Primer Express 2.0 - (reply: 1)
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ChIP: I get smear after PCR? - (reply: 2)
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Old PCR publication - help! - (reply: 2)
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Primers with RE sites - (reply: 2)
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overlap extension using PCR (3way PCR) - (reply: 1)
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normalization for real-time PCR - (reply: 4)
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what can i do with this pcr? - amplification of cDNA , with primers tm 50 and 67 (reply: 7)
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Fusion PCR for eventual TA cloning - (reply: 2)
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improve the DNA cons. in RT-PCR - (reply: 2)
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Inverse PCR problem! plz help! - (reply: 2)
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primer for real-time and reverse transcriptase PCR - (reply: 1)
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reverse transcription PCR - (reply: 1)
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Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
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RT-PCR not working - (reply: 8)
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PCR for >4kb, i got no product. - how shud i optimize my pcr (reply: 9)
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Representing RT-PCR Data - (reply: 2)
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do you ever use PAGE to check PCR? - (reply: 3)
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PCR puzzle- big or small - PCR (reply: 1)
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klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
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explaining competitive PCR - (reply: 1)
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Anyone ever do a molecular beacon PCR? - (reply: 1)
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Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
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Primers going bad - what's the best storage/usage conditions? (reply: 4)
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5' Primer modification - Tm-effect ?? (reply: 1)
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PCR using an enzyme mix - never done this before, how do I set it up? (reply: 4)
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Designing PCR Primers - (reply: 3)
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running a gel with primers - (reply: 2)
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Long PCR on a cosmid vector - Help needed to run PCR (reply: 3)
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the results of allele specific PCR is the other way around - I dont know why (reply: 1)
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Will this primer work? - (reply: 8)
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More PCR cloning problems! - (reply: 12)
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What are the most important parameters in PCR primer design? - Tm difference, GC clamp, GC%, primer-primer complementarity, self-complementarit (reply: 9)
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How much PCR do I need for cloning? - (reply: 5)
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Primers and enzymes not compatible - How can I fix this? (reply: 2)
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cytokines primers - (reply: 1)
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How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
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PCR cloning problems - (reply: 13)
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is there a loss of volume after pcr? - (reply: 5)
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Enzyme Site in reverse Primer - (reply: 3)
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PCR product self life? - (reply: 2)
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general pcr/primer question - (reply: 1)
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dephosphorylated vector and PCR insert - (reply: 7)
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Need a FRET PCR protocol - (reply: 1)
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for the PCR primer what is better the hairpin or the self annealing? - (reply: 9)
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blunt end ligation after inverse PCR - (reply: 5)
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PCR'ing a high GC gene - (reply: 1)
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pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want??? (reply: 5)
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PCR problem with CGG repeats - (reply: 4)
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different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
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Primers for BS treated DNA - (reply: 5)
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Restriction digestion of PCR product - (reply: 3)
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Problems with PCR for GATEWAY - I'd get rid of 100-150bp unspecific band!!! (reply: 5)
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Pfx polymerase for site-directed mutagenesis - (reply: 2)
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Nested polymerase reaction - why to do this nested PCR? (reply: 1)
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RT-PCR product dilution and electrophoresis - (reply: 2)
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forward & reverse primers - (reply: 3)
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How to calculate DNA concentration after PCR manually - not using spectrophotometry or electrophoresis (reply: 6)
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Taq Polymerase storage conditions - (reply: 1)
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pGEM v pTOPO PCR products for sequencing.... Help! - (reply: 3)
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role of ammonium sulphate in PCR buffer - (reply: 2)
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Primers & probes of Applied Biosystems for real time - (reply: 2)
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What is the optimum amount ot bases that can be deleted by PCR? - (reply: 5)
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Can I PCR a gene of 2KB? - (reply: 3)
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When I chage Taq polymerase - (reply: 2)
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Target Amplicon Length for real-time PCR - (reply: 4)
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Primer Optimiztion - can this be done in water (reply: 8)
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question for the PCR to cloning construct - (reply: 9)
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Large fragments from PCR. - problems getting 3.5kb from genomic DNA (reply: 4)
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Mycoplasma PCR detection in media/FBS - (reply: 6)
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increasing sensitivity and reducing primer dimmer - (reply: 11)
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Real-Time PCR Amplicon Question - (reply: 2)
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PCR reagents contaminated w/ bact - still ok for fungi? - (reply: 2)
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ppt DNA directly from a digestion/ pcr - (reply: 2)
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primer design questions for confused - (reply: 2)
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Anyone use phusion HF DNA polymerase? - urgent!!!! (reply: 6)
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How excess template can inhibit the PCR reaction? - (reply: 1)
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Real Time PCR and Gene expression - (reply: 1)
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PCR enzyme activity-can you do 50 cycles - (reply: 2)
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REAL TIME PCR QUESTION - (reply: 3)
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Which primer concentration for RT with very low RNA amounts? - (reply: 1)
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Strange problems with B-actin amplification - (reply: 1)
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forgot to digest PCR insert before ligation. - what happened? (reply: 7)
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restriction site in primers urgent - (reply: 1)
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Preparation of template for PCR - (reply: 7)
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primer design problems - (reply: 4)
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Weird PCR result - (reply: 6)
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problems with PCR from chIP material - double band PCR from chIP material (reply: 2)
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SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
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pcr yield and primer concentration - (reply: 5)
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PCR Setup stopped working - (reply: 14)
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is amplification directly from my ligation reaction possible? - (reply: 1)
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Primer design with restriction sites - (reply: 3)
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Is a 100bp PCR product more prone to give non-specific signals in a hybridisatio - (reply: 1)
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help PCR of GC rich templates - (reply: 3)
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I am so disappointed that I failed in PCR again and again - (reply: 4)
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differences between oligo and random primers - (reply: 2)
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My PCR product is 100bp (approx). What result is more reliable-gel or hybridisat - (reply: 5)
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Why is it difficult to PCR taget gene in rice successfully ? - (reply: 1)
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PCR contamination - (reply: 2)
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Effect of Temperature on PCR - (reply: 3)
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Difficulties getting a 4 kb PCR product - (reply: 5)
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PCR product multiple bands - (reply: 3)
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Any primer design programs...? - (reply: 8)
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Which result is correct-Western or RT-PCR - (reply: 5)
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Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
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PCR problems, huge artifacts, no product - involves 16S primers (reply: 8)
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rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
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Touch down PCR - (reply: 8)
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RealTime PCR of microRNAs - (reply: 9)
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Primer design for BSP - (reply: 12)
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Designing primers and hybridization probes - (reply: 1)
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Junk/dimers on left of single product peak - (reply: 3)
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gel purification of PCR product - (reply: 1)
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RT PCR Primer - Software (reply: 2)
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Possible to clone when you have only 20ng PCR product? - (reply: 10)
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Increased signal in IP after peptide addition? - Immunoprecipitation signal amplification (reply: 2)
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How to PCR a 100bp fragment - (reply: 4)
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Problems with PCR following bisulfite treatment - (reply: 11)
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pBluescript cloning of PCR products - (reply: 1)
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Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
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vector PCR problem - stuck in experiments... (reply: 2)
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please tell me more about primers with restriction site! - (reply: 4)
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Problem in Patch PCR for Site-Directed Mutagenesis - (reply: 5)
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transgenic mouse - identification by PCR (reply: 2)
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pcr detection of pathogens - (reply: 3)
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Recombinant PCR - (reply: 5)
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standard curve and differentiation asays - Real Time PCR (reply: 2)
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[PCR] Different amplification-efficiency of varying DNA - (reply: 1)
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allele-specific amplification - (reply: 1)
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Double bands in PCR - WHY? - (reply: 5)
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Long range PCR - What enzymes do you use? - (reply: 6)
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sear in PCR no bands - (reply: 3)
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Few colonies with cloning of PCR product after digestion - (reply: 7)
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can primers age? - (reply: 1)
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No PCR Product after Bisulfite Modification - (reply: 5)
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PCR with 75 mer forward primer - (reply: 4)
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half life of primers - (reply: 2)
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competition of multiple PCR products - (reply: 1)
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PCR from Genomic DNA - (reply: 3)
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about the primers in MSP - (reply: 5)
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ARMS PCR - (reply: 3)
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cloning problem with pcr and pegfp vector - (reply: 9)
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What annealing temperature is better for my primers? - (reply: 7)
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primer concentration (calculation problem) - (reply: 5)
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Problem with cloning p53-suggestions for RT-PCR - (reply: 1)
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PCR: same premix, same everything, different results... - (reply: 21)
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How to confirm a 80bp PCr fragment? - (reply: 5)
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RT-PCR control for Vitis vinifera - (reply: 2)
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Problem PCR amplifying gene promoter regions - (reply: 4)
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pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
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primer:cross-dimer - How to remove the cross dimer? - (reply: 3)
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PCR trouble.....help! - (reply: 16)
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My PCR's are killing me! - nothing is working!! (reply: 9)
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PCR almost like false positive problem! - How can I get rid of 5bp band near mine !!!!!!! (reply: 2)
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dNTP dilution for PCR - (reply: 4)
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can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
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alternative to dmso in pcr - (reply: 3)
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The weirdest primer ever - (reply: 7)
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Trouble with real-time PCR standard curve - (reply: 4)
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no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
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What happens to PRIMERS at 72 C ? - (reply: 5)
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PCR cleanup - Quick question (reply: 1)
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No PCR product detected in agraose gel - (reply: 7)
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2 melting peaks in real-time PCR - (reply: 1)
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visualizing unknown DNA with linkers and PCR - (reply: 2)
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DNA fingerprinting - Is it necessary to use DNA polymerase with proof-read function in PCR? (reply: 2)
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checking colonies by vector primers.... - (reply: 7)
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Does 2-mercaptoethanol inhibit PCR reactions? - anyone know? (reply: 7)
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Nested PCR troubleshooting - no secondary product - (reply: 5)
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adding restriction sites to degenerate primers - (reply: 9)
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Two to use PCR to get domain deletion within a protein? - (reply: 2)
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Standard vs High fidelity polymerase, please help - No results with high fidelity (reply: 5)
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PCR on a GC rich region - PCR on a GC rich region (reply: 4)
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PCR amplification of Community DNA - (reply: 6)
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Unwanted band seen along with my pcr product. - regarding PCR product with unwanted band formation during electrophoresis (reply: 3)
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cloning primer and tag questions - first try (reply: 2)
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PCR problems with 1,7kb fragment - (reply: 8)
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reuse PCR gel - (reply: 2)
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Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
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Two design programs give very different primer Tm values - RT-PCR (reply: 5)
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Problem of Smear - Smearing of PCR product (reply: 7)
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cDNA synthesis primer - (reply: 3)
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gsamsa - Gene array vs PCR (reply: 4)
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Any one can tell me what happens to my RT-PCR? - Urgent !RT-PCR really kills me! (reply: 5)
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PCR of herbarium specimes - PCR of Caesalpinia herbarium specimes (reply: 1)
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pcr product transfection - (reply: 8)
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restriction digestion of PCR product? - (reply: 10)
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Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
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Taq polymerase vs HotstartTaq polymerase - (reply: 4)
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relative quantification, primer pair problem - (reply: 1)
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Question about PCR reaction condition - (reply: 3)
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PCR Internal Controls - (reply: 2)
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smear in PCR - No consistancy in amplification (reply: 3)
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Designing species specific primers for E.coli - please help? (reply: 3)
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Chloramphenicol amplification - (reply: 5)
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Screening a Gene Library - I want to use colony PCR, but How (reply: 4)
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Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
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Need for Nested PCR - Primers Designed with MethPrimer - (reply: 6)
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single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
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2nd round of amplification issues - (reply: 1)
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Smearing of the PCR Products - (reply: 10)
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Stick T7 on custom primer for sequencing? - (reply: 2)
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SMEAR IN POSITIVE CONTROL IN LONG PCR - (reply: 3)
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Cloning PCR product - Any ideas? (reply: 3)
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MSP primer design help - (reply: 5)
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Primers for nested-PCR - (reply: 1)
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no product after PCR - (reply: 5)
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Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
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making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
-
BSP and MSP Primers - (reply: 2)
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PCR question, need help please! - (reply: 5)
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Bisulfite sequencing primer - (reply: 4)
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specific loci not present depending on DNA extraction method? - DNA extraction and succesful PCR (reply: 1)
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Methods for ligating fragments to form dimers.. - (reply: 1)
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Must all BSP be nested PCR? - (reply: 4)
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QPCR, where to start? - optimizing primer and template (reply: 5)
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about nested PCR - (reply: 2)
-
Primer for sequencing - need help!!!!!!!!!! (reply: 6)
-
Primer design invert pcr - (reply: 5)
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Standard PCR - (reply: 3)
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Left my PCR supplies out overnight. Are they all ruined? - (reply: 4)
-
faster way to identify RNA virus other than RT-PCR? - (reply: 2)
-
Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
-
designing primers for Real Time - (reply: 1)
-
Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
-
Real time PCR late amplification - (reply: 6)
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PCR anealing temperature question - (reply: 12)
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Taq and Pfu polymerase give different amplification - (reply: 8)
-
Methods for primer reconstitution - (reply: 23)
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Help on PCR purification - (reply: 2)
-
BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
-
Troubleshooting PCR contamination - (reply: 13)
-
no result with pcr. is it the primer?/ - no amplification in pcr even after starting from the beginning (reply: 6)
-
PCR result: Nothing! - Urgent! (reply: 2)
-
PCR large fragments - (reply: 2)
-
can we add the terminaton codon in the 3' primer? - (reply: 1)
-
PCR probes for Southern blot - (reply: 1)
-
Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
-
designing primer for methylation - (reply: 21)
-
Primer dimers?Contaminations? - Unexpected bands! (reply: 5)
-
Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
-
Use PCR products to determine efficiency of amplification? - (reply: 7)
-
Band in negative PCR control - (reply: 25)
-
question in 5' RACE and primer extesion - (reply: 8)
-
Primer concentration ? - (reply: 2)
-
PCR my cDNA & got nothing - (reply: 2)
-
PCR product dimer (not primer dimer!) - PCR (reply: 2)
-
Degradation of PCR product during purfication - Purification of PCR product for cloning (reply: 2)
-
Nonspecific PCR amplification? - (reply: 2)
-
Plasmid PCR - (reply: 5)
-
Nested allele-specific PCR - (reply: 1)
-
problems in subcloning PCR products for TA cloning - (reply: 4)
-
no product for promoter genomic PCR - (reply: 3)
-
from which strand to derive BSP primers? - (reply: 5)
-
SMear instead of PCR product - (reply: 3)
-
Best product size for real-time RT-PCR - (reply: 4)
-
what is the principle of gradient PCR? - How does it work (reply: 2)
-
Why is my smaller band too faint? Genomic PCR not working! - Just trying to genotype some mice... (reply: 2)
-
Removal of PCR inhibitor - (reply: 3)
-
Both the PCR primers in a vial. - Did anybody mix the diluted primers in single tube for use? (reply: 7)
-
Concentrating PCR product - (reply: 9)
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How to design primer for sequencing unknown DNA - (reply: 5)
-
PCR mix and DEPC water - (reply: 2)
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need helps on probe design--human ip10 primers and probe. - (reply: 2)
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PCR - (reply: 8)
-
Success with Direct Sequening of Bisulfite Pcr Products - (reply: 47)
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how to determin the Annealing Temp of ristriction sites modified primer? - help me ! (reply: 26)
-
two bands of one gene in PCR - (reply: 8)
-
RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
-
About PCR primers' concentration - (reply: 2)
-
Multiple unknown amplification products - (reply: 2)
-
Qiagen Gel Extraction of PCR product - (reply: 7)
-
Problem with lids to 8-strip .2 ml PCR tubes - (reply: 3)
-
Measuring the amount of PCR product - (reply: 9)
-
difficult PCR - (reply: 1)
-
Low PCR product for antibody gene - (reply: 4)
-
Colony PCR positive, miniprep negative? - (reply: 9)
-
RT-PCR machine/setup problems - (reply: 2)
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Primer dimers - (reply: 1)
-
troubles with human genomic DNA amplification - (reply: 1)
-
RT-PCR question - genomic DNA amplified with RNA (reply: 9)
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Preserve PCR mix - (reply: 3)
-
problem concerning PCR Test after disruption and yeastgenome DB - (reply: 6)
-
primers design for mutagenesis of two nearby sites - mutations (reply: 2)
-
Asymmetric PCR - (reply: 2)
-
PCR of long fragment(approx 8 kb) - (reply: 3)
-
PCR..HELP - band in no template control (reply: 1)
-
Troubleshooting PCR smear - Urgent,Need help! (reply: 4)
-
[urgent help] Band missing in PCR - (reply: 5)
-
Multiplex PCR - (reply: 4)
-
Smearing in nested pcr product - (reply: 2)
-
Intensive primer dimers - (reply: 2)
-
PCR using different primers to generate same DNA sequence - explanation please :) (reply: 2)
-
PCR no longer works - no pcr product? (reply: 3)
-
What should I do for more samples need to be tested by real-time PCR? - (reply: 1)
-
Laddering effect in PCR blank control - (reply: 4)
-
Do you synthsize your own oligo(dT) primer? - (reply: 2)
-
PCR on Genomic DNA - (reply: 2)
-
Could higher ammount of taq be inhibitory for overall PCR reaction? - (reply: 2)
-
genotyping mice-no pcr product - (reply: 3)
-
Nested PCR - no amplicon - (reply: 1)
-
PCR - (reply: 3)
-
Volume of Real time PCR mastermix and reaction - (reply: 4)
-
Weird duplex PCR results - (reply: 5)
-
PCR - (reply: 4)
-
potassium acetate in primer solution - I made a mess but need to recover (reply: 6)
-
cloning/amplifying attenuate RNA transcripts - (reply: 6)
-
Nested PCR problems - (reply: 1)
-
confirmation of my pcr product - (reply: 2)
-
16S rRNA primers for E. coli - (reply: 2)
-
About PCR smear - Anyone can help me? (reply: 4)
-
primer design - how to design primers (reply: 2)
-
Real-time PCR problems - RT_PCR (reply: 7)
-
HIV RT PCR KIT - (reply: 1)
-
Real time PCR - no product - (reply: 1)
-
Multiplex Primers - Primer primer interaction (reply: 1)
-
Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
-
PCR smear in samples and negative control - (reply: 5)
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How should I check my colonies? - PCR check is not working...:( (reply: 5)
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question about mutagenic PCR. - (reply: 2)
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phenol/chloroform of purified PCR product after RE digestion - what are we trying to purify? (reply: 4)
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PCR and topo problem - (reply: 3)
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Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers? - (reply: 6)
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How to calculate absolute fold change in expression by realtime PCR - Realtime PCR (reply: 2)
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Amplification of 3KB protein from Hela mRNA PCR PROBLEM - PCR Amplification of 3KB protein (reply: 6)
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qPCR for HKG and traditional PCR for GOI - (reply: 1)
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Smeary bands in real-time RT-PCR - (reply: 7)
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RT PCR - (reply: 1)
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How to purify PCR product? - (reply: 3)
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PCR internal control - how does it work? (reply: 1)
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False positive for PCR screening of insert - (reply: 1)
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why using Vent-exo polymerase? - (reply: 2)
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Programs for designing Primers - (reply: 6)
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Disadvantages of using oligo-dT primers - (reply: 1)
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Selective amplification problem in AFLP - (reply: 1)
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my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
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Do I need internal controls in MS-PCR? - Methylation specific PCR Internal controls (reply: 3)
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PCR Trouble shooting - PCR Trouble shooting (reply: 2)
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ES cell genomic DNA as PCR template for screening - (reply: 5)
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Problem with PCR amplification - (reply: 2)
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Simple PCR - (reply: 1)
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targetted pcr - targetted pcr (reply: 3)
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Sequencing primer dilution - (reply: 2)
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PCR for cloning - PCR (reply: 2)
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PCR with degenerate primer - (reply: 6)
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1.3kb PCR only shows primer dimer - (reply: 2)
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Titration of Mg2+ in RT-PCR - (reply: 3)
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RT-PCR primers for E. coli LacZ gene - (reply: 3)
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SOS: real time PCR standard curve - (reply: 1)
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Serratia PCR - (reply: 6)
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QuickChange mutagenesis: how to do it without kit? - What dNTP concentration should I use? (reply: 1)
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Long distance (16 kb) PCR problem - (reply: 12)
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Primer for 4 genes sharing short consensus sequence - (reply: 2)
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Looking for online primer design for SNaPshot - (reply: 1)
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ask for an explanation to pcr ladder at high cycle number - help (reply: 2)
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PCR primers: hairpin and dimers? - what can I do to avoid this? (reply: 4)
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BSP - favors amplification of unmethylated template? - (reply: 2)
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PCR on PCR of cDNA smear - (reply: 1)
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Reconstitute primers - (reply: 4)
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PCR product - (reply: 5)
-
Delta-delta Ct method and PCR efficiencies - (reply: 9)
-
multiplex PCR for three sets of primers - (reply: 1)
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repeat PCR amplification problem - (reply: 2)
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to purify these PCR products or not? - preparation for restriction digest (reply: 5)
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How many mismatches in point mutation primers? - (reply: 3)
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Software available for Multiplex PCR primer designing - (reply: 3)
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PCR of ligation products - (reply: 8)
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multiplex pcr trouble - some bands won't amplify (reply: 16)
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Subcloing PCR fragments - (reply: 1)
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3' - 5' activity of Taq DNA Polymerase - (reply: 2)
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Amplify 4.5 kb pcr product without using special polymerase - (reply: 10)
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SOS! asymmetric PCR, no PCR product - (reply: 2)
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RT-PCR- help - Polyacrylmide Gel electrophrosis for PCR products (reply: 3)
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Mouse MSP primers - (reply: 11)
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Primer Dimer problems in real-time PCR - (reply: 14)
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primer orientation - (reply: 1)
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Degenerate primers for nested PCR? - (reply: 1)
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Looking for real time PCR workshops - real time PCR workshops (reply: 2)
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Reliable ChIP primers for Sp1? - (reply: 2)
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increased primer dimer with template - (reply: 2)
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How long should the DNA fragment be amplified by the two-step PCR? - (reply: 8)
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primer efficiency in real time PCR - need for a method explanasion (reply: 1)
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PCR High GC content - PCR High GC content (reply: 11)
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PCR, contamination or not - (reply: 1)
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design of PRC primers of full ORF - (reply: 3)
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Two round MSP with the same primer - (reply: 1)
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faint pcr bands to some, intense to some - (reply: 5)
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Designing a Primer within an exon - Consesus required - (reply: 2)
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Contamination/no amplification in MSAP-PCR - (reply: 3)
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No bands at all in gel for PCR products - (reply: 5)
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Finding PCR Limiting Reagent - (reply: 5)
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problem wiht long pcr fragment - (reply: 2)
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difference between primer extension and RACE - (reply: 6)
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Is RealTime PCR data enough for Telomerase activity? - (reply: 4)
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No insert for cloning PCR product into expression vector - (reply: 5)
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designing QRT-PCR Primers - (reply: 11)
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multiplex PCR - (reply: 4)
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How can I get the 4.5kb Human typeI procollagen alpha1 chain cDNA Via RT-PCR fr - Seeking Help (reply: 14)
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PCR Smear - (reply: 2)
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More RT PCR questions: - can I amplify "everything?" (reply: 2)
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Ethidium Bromide vs Sybr Green stain (normal PCR) - (reply: 5)
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PCR master Mix problem...Plz help me out - PCR (reply: 8)
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RT-PCR on plasmid - (reply: 1)
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Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
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Detection of Bt gene in Corn Through PCR - High School Science Fair - (reply: 6)
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separation of pcr products of similar sizes in agarose gel - (reply: 1)
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Can I add additional sodium ion to PCR reaction buffers? - (reply: 5)
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PCR product precipitation - (reply: 4)
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Cloning PCR product - double bands or heterozygote (reply: 5)
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virtual PCR - (reply: 3)
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Real-time PCR machines for DNA quantitation - (reply: 5)
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purification by gel? - after a first round of PCR (reply: 2)
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Does Reverse Transcription have to be done using PCR - (reply: 6)
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how to improve the efficiency of my FQ-PCR - I can not get a beautiful amplification curve (reply: 3)
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PCR of AmpR gene - PCR of Amp gene (reply: 1)
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PCR reactions - What condition should I use? (reply: 5)
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inverse PCR or promoter trapping - (reply: 2)
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Taq polymerase - (reply: 5)
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Methylation & PCR - (reply: 1)
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TaqMan probes, pcr mix - (reply: 1)
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What way would you suggest to clean PCR products? - (reply: 4)
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Primer design question - Use of primer 3 (reply: 3)
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Long PCR for amplification from plasmid - (reply: 5)
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PCR after Sonication... - (reply: 10)
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PCR trouble - (reply: 16)
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cloning of PCR product - (reply: 1)
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KOD plus for PCR - (reply: 1)
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RT-PCR Primer design - which region? - (reply: 2)
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Lower size band in genomic PCR with 300 bp missing - (reply: 12)
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PCR amplicon size and contamination - (reply: 4)
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Gelatin for PCR - (reply: 2)
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Question about gel purification of PCR product - (reply: 1)
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nested PCR - (reply: 3)
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No PCR products in MSP with MethPrimer designed primers - (reply: 2)
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Taq polymerase dilution? - (reply: 3)
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help pcr cloning problem - help pcr cloning problem!!!!!1 (reply: 2)
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problems with mix of MSP's primers - (reply: 2)
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[PCR products] - (reply: 1)
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real time PCR with sybr green - having problems validating my knock down (reply: 3)
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PCR an insert in a vector with wrong product size - (reply: 1)
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RNA extraction with TRizol and RT-PCR - (reply: 8)
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design primer for PCR.. - (reply: 3)
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Amplitaq Gold pcr master mix - Protocol question (reply: 1)
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realtime pcr validating the results of microarray? - (reply: 7)
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gel extraction of pcr products - (reply: 3)
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PCR for more than 5 kb - (reply: 4)
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MSP primers not working - (reply: 1)
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Quick cloning question - Purification of PCR fragment (reply: 1)
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problems with bisulfite PCR - (reply: 8)
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extra sequence in primers - (reply: 4)
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Amplification problems - (reply: 9)
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Sybr RT PCR problem - (reply: 4)
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calculating Tm of primers - (reply: 8)
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AmpliTaq Gold instead of TaqMan Universal PCR Mix in Real-time? - (reply: 3)
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RT-PCR for large fragment - (reply: 7)
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nested PCR after bisulphite - (reply: 3)
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design pcr primers - (reply: 3)
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RT-PCR problem - negative control (reply: 1)
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Random genoic amplification for microarray - (reply: 4)
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Real-time PCR - contamination or not? - (reply: 6)
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Degenerate PCR troubleshooting - (reply: 3)
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A problem of quantitative PCR - (reply: 2)
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maximum size for PCR - (reply: 3)
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Bisulfite treated DNA specific primer set critique? - (reply: 18)
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60 bp PCR and primer dimers - (reply: 6)
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faint bands of PCR products - (reply: 5)
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PCR contamination with human DNA - (reply: 5)
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bisulfite modification after pcr - (reply: 2)
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Degenerate PCR troubleshooting - (reply: 9)
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effect of ethanol and dna concentration in pcr - (reply: 3)
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PCR small framents - (reply: 2)
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real time PCR (inconsistant results) - (reply: 2)
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PCR optimization - (reply: 12)
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no band obtain after PCR - (reply: 2)
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Problem with PCR - (reply: 4)
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primers for SoE - (reply: 1)
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Trouble with Inverse PCR - (reply: 8)
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multiple band on PCR - (reply: 5)
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PCR enhancer - (reply: 5)
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Discussion: ChIP PCR control - (reply: 12)
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designing flanking primers around msp primers - (reply: 1)
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Help: (PCR) Increase template, but NO Increase in product - (reply: 5)
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PCR purification basics? - (reply: 6)
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No PCR products with the ChIP DNA - (reply: 5)
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primer does not bind - help (reply: 2)
-
reconstitute primer - (reply: 4)
-
RT-PCR scale-down makes more sensitive? - (reply: 3)
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Purification of short PCR product - (reply: 4)
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PCR Supermix vs Roche - (reply: 3)
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SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
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PCR contamination by recombination - (reply: 1)
-
RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
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T4 DNA poymerase - T4 DNA polymerase (reply: 5)
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Unmethylated primer control - (reply: 4)
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Cloning large pcr product - efficiency (reply: 2)
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how much total RNA do you use for RT-PCR - (reply: 6)
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Extremely PCR scale down - (reply: 4)
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mastermix for Real-time PCR - (reply: 2)
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PCR from genomic DNA - (reply: 1)
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PCR on Leishmania DNA - (reply: 1)
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Buffers for storing and diluting of dNTP - (reply: 3)
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Help a first time cloner with PCR! - First time cloning (reply: 5)
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Primers for RT reaction - (reply: 6)
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Real Time PCR - Relative Quantitation - Choice of calibrator and calculations (reply: 1)
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PCR product size with a Roche Lightcycler? - (reply: 1)
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Primers design: how to check for mispriming? - (reply: 2)
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PCR from genomic DNA - (reply: 6)
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standard curves - real time pcr (reply: 3)
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EDTA - real time PCR (reply: 3)
-
Pls check my bisulphite sequencing primers - (reply: 12)
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Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
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How to setup control for bisulfite sequencing PCR - (reply: 2)
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Bad Primer - (reply: 2)
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PCR problems - (reply: 3)
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delta delta Ct method - Real time PCR (reply: 4)
-
Reverse-transcriptase PCR troubleshoot HELP - (reply: 13)
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PCR troubleshooting - Primers (reply: 3)
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Calculating correct melting temperature for primers - (reply: 2)
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How can I add sequences to DNA using PCR? - (reply: 1)
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end of primer - (reply: 2)
-
T/A cloning of cDNA? - PGEM-T vs pCR 2.1 T/A TOPO? (reply: 3)
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PCR primer dimer? - (reply: 7)
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How many times for a run -real time pcr - (reply: 1)
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How to avoid secondary structure in primer - (reply: 2)
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subcloning of PCR product, please help! - subcloning problems (reply: 12)
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Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
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real-time PCR - (reply: 5)
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Realtime PCR standard curves - (reply: 8)
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Real time PCR supermix - (reply: 4)
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PCR contamination mystery - (reply: 2)
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Real-time PCR amplification curve up and down - (reply: 3)
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Which RT primer is better for qPCR - (reply: 2)
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HEPA filter - and PCR contamination (reply: 1)
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Problem with amplifying DNA from FTA cards - Is there a special trick involved? (reply: 1)
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Why different amounts of forward and reverese primers in RT-QPCR - (reply: 2)
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primer optimization - (reply: 1)
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PCR on plasmids? - (reply: 2)
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Pfx PCR problems - (reply: 1)
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Storage of RT-PCR products - (reply: 2)
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PCR of a large DNA fragment - (reply: 3)
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Pipetting on ice - during PCR (reply: 5)
-
Sometimes i get bands, sometimes i get nothing for my PCR - Strange PCR Results (reply: 1)
-
Multiplex PCR troubleshooting - (reply: 6)
-
Real Time qPCR - qPCR SYBR greenI primer design (reply: 5)
-
Primers for species identification - Know any good? (reply: 6)
-
Genotype ratio with quantitative PCR - (reply: 1)
-
can't get rid of DNA contamination even with DNase for RT-PCR - (reply: 3)
-
PCR master mix or super mix - PCR technique (reply: 1)
-
PCR screeing for positive transormation - (reply: 1)
-
Contamination in PCR cloning - (reply: 3)
-
PCR fragment analysis - please help! - PCR fragment analysis - please help! (reply: 2)
-
Hot start PCR - (reply: 6)
-
mutilple bands after RT-PCR - (reply: 2)
-
How to measure the concentration of primers? - (reply: 2)
-
Probes or primers that span introns - (reply: 6)
-
Problem with second PCR - 1st PCR product and 2nd PCR product are different (reply: 1)
-
Cloning a large PCR product - trouble cloning large DNA (reply: 3)
-
real-time pcr plasmid standards - (reply: 4)
-
How much of the starting amounts for Real time PCR? - (reply: 1)
-
PCR parameters - (reply: 2)
-
Unequal amplification efficiency of heat blocks - (reply: 4)
-
PCR colony screening - (reply: 9)
-
help regarding primers reconstitution - (reply: 4)
-
PCR - Long PCR with short primers (reply: 1)
-
Calculating Tm of a PCR product? - (reply: 6)
-
Sequencing of PCR products - (reply: 5)
-
a Takara TA taq DNA polymerase protocol - (reply: 1)
-
how long a primer should I use? - (reply: 1)
-
problem with PCR --using fragment as template - (reply: 12)
-
real time PCR kit components - (reply: 2)
-
identifying exon and intron for primer design - (reply: 2)
-
PCR Genomic DNA - PCR Genomic DNA (reply: 1)
-
PCR question - (reply: 3)
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Real Time PCR and melting curve - (reply: 2)
-
threshold for real time PCR - (reply: 1)
-
TA cloning with short PCR products - use of TA cloning kit (reply: 4)
-
How do you prepare PCR stock solution to minimize pipetting - (reply: 37)
-
multiplex PCR for 9 exons - (reply: 5)
-
Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system? (reply: 1)
-
tRNA and Real time RT-PCR - (reply: 1)
-
Designing methylation primers on long CpG island - (reply: 14)
-
3 bands on RT-PCR - (reply: 4)
-
DNaseI have affect with RT-PCR or not - (reply: 3)
-
RT- PCR? - (reply: 2)
-
real time PCR primer for methylation - (reply: 2)
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Determine the RNA concentraiton for linear PCR - (reply: 1)
-
contamination in real-time pcr negative control - (reply: 12)
-
TOPO TA pCR 2.1 cloning - (reply: 1)
-
Real-time PCR problems - (reply: 3)
-
software to find restriction site and TM for primer - (reply: 7)
-
Large scale PCR to get milligram of DNA - (reply: 6)
-
real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
-
PCR problem: DISTINCT bands far shorter than target! - (reply: 3)
-
genotyping transgenic mice with PCR - (reply: 1)
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Failed to amplify a 2.1 kb PCR product - (reply: 3)
-
About PfuTurbo® DNA Polymerase and Pfu DNA Polymerase - (reply: 1)
-
realtime RT-PCR/internal control - (reply: 3)
-
PCR LABORATORY - to avoid contaminations... (reply: 1)
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PCR contamination - (reply: 1)
-
PCR low amplification - (reply: 2)
-
RT-PCR gone bad - (reply: 4)
-
Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
-
Which vector for cloning big PCR fragments? - (reply: 3)
-
microsatellite PCR from poor quality/trace amounts of DNA - (reply: 3)
-
Asymmetric PCR for generation of single stranded DNA - (reply: 5)
-
GC content of PCR primers - (reply: 3)
-
influence of MgCl2 in PCR - (reply: 7)
-
RT-PCR - (reply: 1)
-
cpgware primer design, does it work - (reply: 4)
-
RT-PCR - 2 kb fragment in RT-PCR (reply: 2)
-
Who will give me a software to design primer for PCR? - (reply: 3)
-
No PCR Product for Sequencing - (reply: 5)
-
PCR - No bands anymore in well functioning PCR - (reply: 6)
-
problems with PCR long target gene - (reply: 2)
-
No PCR Product - (reply: 2)
-
PCR comparison of gene expression - (reply: 5)
-
"PCR ghosts" - :wacko: (reply: 2)
-
Sequence input for bisulfite PCR primer design - (reply: 1)
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Gradient PCR - (reply: 2)
-
PCR Failure..pls help! - (reply: 3)
-
Real Time PCR Annealing vs. Melting - Relation of anneal temp rxn to melt temp primer (reply: 2)
-
Is there a PCR length limitation after Bisulfite treatment? - (reply: 3)
-
RT-PCR with B-cells as template - (reply: 1)
-
PCR for ChIP - PCR for ChIP not working (reply: 3)
-
primer design for bisulphite treated DNA - (reply: 1)
-
PCR more difficult in methylated CpG region? - (reply: 7)
-
PCR of unknown DNA - PCRing unknown DNA with known linkers (reply: 2)
-
Quantification of PCR using delta-delta Ct method. - Quantification (reply: 1)
-
ligation of pcr product - (reply: 4)
-
TAQ polymerase is needed - TAQ polymerase for 60 cycles (reply: 1)
-
How to determine PCR annealing temperature - (reply: 9)
-
sometimes I get it sometimes I dont - My PCr does not work always (reply: 9)
-
allele-specific PCR - detection of SNPs by allele-specific amplification (reply: 4)
-
How to remove PCR inhibitors from DNA - (reply: 7)
-
Problem cloning PCR product into double-cut vector - (reply: 3)
-
RT-PCR primer design - exon-exon primer design (reply: 5)
-
Primer design - PCR for cloning (reply: 6)
-
Promoter amplification.... - (reply: 7)
-
1-2kb PCR products TOPO cloning troubles - help (reply: 4)
-
RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (reply: 1)
-
PCR product for ligation - no PCR product from PCR product (reply: 1)
-
availabre primers for MSP - (reply: 1)
-
Strange mobility behavior of PCR products on agarose gel - EB stained vs Gelstar stained (reply: 2)
-
PCR or RT-PCR? - (reply: 3)
-
3' end of forward primer mismatch - (reply: 5)
-
Problem with PCR on difficult template - How to amplify GC-rich region? (reply: 6)
-
DNA quantification - How to quantify my PCR product before sequencing?? (reply: 8)
-
RACE - specific primers in first strand synthesis (reply: 4)
-
cloning PCR fragment - PCR fragment is not getting cloned in the vector (reply: 2)
-
checking for inserts with colony PCR - (reply: 6)
-
Primer Designer Online - Pipleline use of designer (reply: 1)
-
PCR-Check, Screen-Check, MiniPrep - HUH?? - PCR product transformed, grown on AMP, no mini (reply: 2)
-
Cloning a long PCR product - (reply: 2)
-
If the primer is dimer,it's ok? - (reply: 5)
-
Troubleshoot PCR smear problem - all I got was smear!! (reply: 71)
-
cloning size is not correct - 2 bands on the PCR product (reply: 2)
-
Multiplex PCR primer designing - (reply: 4)
-
still more about cloning PCR products - (reply: 9)
-
Annealing temperature for PCR - (reply: 3)
-
Genomic DNA amplification - (reply: 4)
-
RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
-
PCR is6110 Tuberculosis - Non specific amplification (reply: 2)
-
Bisulfite modification kits and PCR - (reply: 5)
-
Transformation and PCR questions - (reply: 2)
-
PCR contamination in negative control - (reply: 3)
-
PCR Cloning - primer design and CpG methylation (reply: 4)
-
cloning from PCR - (reply: 25)
-
sowing pcr? - (reply: 5)
-
How to identify plasmid - using enzyme and PCR or other? (reply: 2)
-
BiPolar Clamps on PCR primers for TGGE - (reply: 1)
-
multiplex primers - (reply: 6)
-
Bisulfite PCR: Smear - Can't amplify a certain sequence (reply: 4)
-
PCR product sequencing BD v3.1 - Help in removal of residues (reply: 1)
-
RNA and DNA extraction and PCR control - (reply: 1)
-
About Q-PCR(real-time PCR) - COMPARE (reply: 1)
-
dissociation curve of real time pcr products - 2 different peaks in dissociation curve (reply: 2)
-
parse primer3 output - (reply: 3)
-
bisulfite pcr primers - (reply: 1)
-
The PCR Suite - a set of new primer design tools - (reply: 1)
-
pcr ligation - problems with ligation (reply: 1)
-
PCR problem - can not get the target SSR band (reply: 1)
-
RT-PCR amplification problem - amplification problem (reply: 1)
-
PCR problems - (reply: 4)
-
Is there anybody use Taq polymerase of Fermemtas - (reply: 20)
-
Amplification Problem - PCR Failure... (reply: 5)
-
LacZ PCR - (reply: 1)
-
Fusion PCR Protocol? - (reply: 6)
-
Primer dimers - (reply: 1)
-
RT-PCR 1 step - (reply: 2)
-
PCR and Primers - PCR-no products (reply: 4)
-
AMPLIFYING LONG FRAGMENTS - problems obtaining 3kb fragment (reply: 4)
-
Amplification Failure of Unknown Reason - (reply: 1)
-
Ammonium Sulfate PCR buffer - Ammonium Sulfate PCR buffer (reply: 1)
-
Detection in SmartCycler RT-PCR - (reply: 1)
-
Probes for Real-Time PCR - (reply: 3)
-
DNA Contamination in RNA? - PCR of RNA (reply: 1)
-
Help! DNA contamination in RNA and RT-PCR. - (reply: 1)
-
Problems in my Multiplex PCR - (reply: 6)
-
PCR detection of water-bornebacteria - (reply: 1)
-
Sequencing PCR products - (reply: 2)
-
cDNA PCR - Amplifying region using PCR from cDNA library (reply: 1)
-
tomato DNA for PCR - (reply: 1)
-
Re: dNTP make up - (reply: 1)
-
My PCR is not work again - (reply: 6)
-
PCR from parraffin embeded samples - (reply: 2)
-
Primer design - Primer design for RT-PCR (reply: 1)
-
Difficult PCR amplification of 4kb genomic DNA - (reply: 3)
-
Primer contamination - (reply: 2)
-
PCR on cDNA libraries - (reply: 2)
-
Blunting 3' ends with T4 polymerase - (reply: 1)
-
Difference between RNase Protention and RT-PCR - (reply: 1)
-
A variation of the usual PCRprotocol needed - (reply: 3)
-
RNase and PCR - (reply: 1)
-
pcr mutagenesis - (reply: 3)
-
design a primer - how to determind a specific primer in a complete gonome? (reply: 1)
-
dUTP used for PCR - (reply: 6)
-
PCR with cell source - (reply: 1)
-
In situ PCR and hybridization in plant - In situ PCR/hybridization protocols (reply: 1)
-
Difference in RT PCR products when using total RNA or mRNA - (reply: 4)
-
PCR mutagenesis - (reply: 1)
-
M13 phage amplification,who canhelp? - (reply: 2)
-
Poor PCR result - (reply: 2)
-
Rt-PCR help,come in - use RT (reply: 2)
-
PCR or Southern? - (reply: 3)
-
hot start Taq - Taq polymerase (reply: 1)
-
How to find/check the highly conservative sequence - RT-PCR primers (reply: 2)
-
RT-PCR - (reply: 2)
-
pcr on genomic dna ( tailsmouse), HELP !!!! - (reply: 2)
-
Bioinfomatics (ePCR) - (reply: 1)
-
About Marker - for PCR (reply: 2)
-
Trypsin - PCR amplification (reply: 1)
-
PCR problem - non specific bands - pcr problem (reply: 8)
-
Poor PCR Results - (reply: 3)
-
ABI377 Sequencing of PCR products - (reply: 1)
-
How to clean agar contamination for colony PCR - (reply: 2)
-
PCR problems - (reply: 1)
-
PCR restriction site - (reply: 2)
-
Single-cell RT-PCR with only ~10-100 Cells - (reply: 2)
-
C-MYC AND CYCLIN D1 / RT-PCR - (reply: 2)
-
a question about RT-PCR - (reply: 1)
-
primer purification - search method for primer purifying (reply: 3)
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How to make PCR using the gel solution - (reply: 1)
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Software for MSP (methylation-specific PCR) primer design - (reply: 3)
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primer design - Deinococcus (reply: 1)