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  1. PCR product appear two close band in my gel! - (reply: 3)
  2. Multiplex pcr trouble - (reply: 4)
  3. need help...how to compare mouse vs human sequences for primer design - (reply: 2)
  4. RT-PCR for small RNAs - (reply: 1)
  5. DNA PAGE - suitability of PCR buffer (reply: 1)
  6. pcr amplification - right amplification,right sequence but still in dilemma (reply: 4)
  7. Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
  8. Tm of PCR primers: one 68, the other 58. Impossible to succeed? - (reply: 3)
  9. PCR product dimer - clean it up (reply: 3)
  10. Primer design for PCR following ChIP - (reply: 3)
  11. PCR Inhibitor - Where/What are you? (reply: 9)
  12. Primer dimers!? HELP - (reply: 8)
  13. MSP primer does not work - (reply: 5)
  14. How to increase the product amount in BSP? - weak amplification in BSP (reply: 3)
  15. general PCR question - (reply: 15)
  16. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
  17. PCR product stuck in agaros wells - (reply: 8)
  18. how 2 design rev.transPCR primer? - need functioning protocol.... (reply: 1)
  19. methylnick.please help me with MSP primer designing! - (reply: 3)
  20. PCR efficiencies for analysis of relative real time data - (reply: 1)
  21. End of PCR products destroyed - (reply: 2)
  22. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
  23. Inverse PCR ligation - (reply: 1)
  24. Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
  25. deisgning primers with restriction site - (reply: 7)
  26. RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
  27. cDNA primer help - (reply: 1)
  28. Background amplification - (reply: 1)
  29. Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
  30. Primer design program for Methylight - (reply: 3)
  31. repeting PCR failing the reaction - (reply: 2)
  32. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
  33. PCR efficiency? - (reply: 3)
  34. PCR fragment ligation - ligation of 3 pcr frangments (reply: 1)
  35. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
  36. Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
  37. PCR false positive reasons - (reply: 3)
  38. PCR cycle - (reply: 1)
  39. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
  40. Why am I only getting smears for my genomic DNA long-distance PCR products? - It worked great with sheep BACs but failed miserably for genomic DNA (reply: 4)
  41. PCR amplification - (reply: 2)
  42. primer sequence - reverse primer sequence (reply: 3)
  43. RT-PCR GAPDH Variability - (reply: 1)
  44. PCR Optimization Question - Non-specific smearing around target level? (reply: 6)
  45. PCR troubleshooting - (reply: 10)
  46. pcr question - (reply: 5)
  47. PCR bands - why not straight ?&%#@&& - (reply: 3)
  48. how to optimize rt-pcr for gene expression analysis - (reply: 3)
  49. PCR negative control contaminated and not the other wells - (reply: 4)
  50. RNA extraction for real time PCR from specific brain region - (reply: 1)
  51. PCR Problem - 300bp skip in the middle (reply: 9)
  52. Designing Primers - (reply: 1)
  53. Immunostaining - Amplification of signal - (reply: 1)
  54. rolling circle amplification - (reply: 1)
  55. Normal PCR for looking expression - (reply: 1)
  56. internal control primers for plants - (reply: 14)
  57. Where do you get your PCR primers from? - (reply: 3)
  58. genomic DNA contamination in RT-PCR - (reply: 7)
  59. how much cDNA do you use for RT-PCR? - (reply: 2)
  60. PCR WOES...... - STRATEGY DOUBT... (reply: 3)
  61. Strange amplification pattern in DNA real time PCR - (reply: 4)
  62. Could have more DNA concentration inhibition effect on PCR? - (reply: 3)
  63. BLAST Help , Designing primers... - (reply: 5)
  64. pyrophosphate generation in PCR - (reply: 2)
  65. housekeeping gene in klebsiella pneumoniae for RT PCR - (reply: 2)
  66. RT PCR of viral RNA segment - (reply: 1)
  67. Primer design and degeneracy - Are synthesized sets identical? (reply: 2)
  68. panomics promoter methylation PCR kit - results not what i expected (reply: 1)
  69. primers amplify additional band (!) - (reply: 1)
  70. resuspended primers in water - is this a problem?! (reply: 5)
  71. Vent polymerase vs Klenow - (reply: 1)
  72. Designing the primers for qRT PCR - Query - (reply: 14)
  73. primers sequence - (reply: 2)
  74. Chip Troubles - Help: Strong PCR signal from negative control region (reply: 7)
  75. primer designing - (reply: 1)
  76. wierd rt-pcr - (reply: 1)
  77. genomic DNA PCR - is it the same as a cDNA PCR (reply: 9)
  78. spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
  79. Unspecific PCR bands - Why that happens? (reply: 5)
  80. Primer doubts - (reply: 2)
  81. PCR cloning - (reply: 5)
  82. Fast vs Standard PCR - (reply: 1)
  83. RT-PCR to amplify ORFs - (reply: 3)
  84. Transcript variation - measuring by RT-PCR - (reply: 3)
  85. Problem with promoter PCR and cloning - (reply: 3)
  86. PCR components/DNA sample optimal amounts - ul of DNA and each component for a good reaction. (reply: 10)
  87. please help me to be sure from design primer have it - (reply: 3)
  88. heparin in DNA - no working PCR (reply: 4)
  89. when to set up a RT-PCR for mRNA expression? - detect transcription of transgene (reply: 1)
  90. When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
  91. Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
  92. resuspend Primers in TE or in water? - (reply: 7)
  93. ssr cross species amplification - (reply: 2)
  94. Can we measure gene expression with standard PCR? - (reply: 1)
  95. Abgene PCR master mix?-MgCl concentration - (reply: 1)
  96. Sybr Green real time pcr limit of detection - (reply: 1)
  97. Taq polymerase expressing E. coli requested - (reply: 2)
  98. BLAST search of primer sequence - (reply: 3)
  99. Best house keeping gene for RT-PCR - (reply: 6)
  100. RT-PCR inconsistant results - (reply: 1)
  101. Oligo dT primer - oligo dT primer with non-T priming 3´ or with 5´ (reply: 3)
  102. PRC reagents and equipments - Is a PCR machine robust? (reply: 2)
  103. Large smear in PCR - (reply: 2)
  104. developing real time PCR - (reply: 5)
  105. false positives in PCR due to environmental contamination? - (reply: 7)
  106. Primer design - How to - am a novice ? (reply: 6)
  107. Primer optimization (with probes) - Urges... (reply: 2)
  108. arms PCR problem - (reply: 2)
  109. storing pcr samples - (reply: 19)
  110. RT-PCR reagents KIT 1 or 2-step? - (reply: 3)
  111. Extraction of PCR product from gel - (reply: 4)
  112. Choice of one-step or two-steps RT-PCR - (reply: 3)
  113. Problems with Bisulfite PCR - (reply: 13)
  114. Choosing Primer: random or Olig-dt ? - (reply: 2)
  115. how to synthesize the pcr primers - (reply: 3)
  116. Washing the spin columns from PCR purekit - (reply: 1)
  117. Trouble inserting PCR derived insert into vector - Trouble inserting PCR derived insert into vector (reply: 2)
  118. RT-PCR primer design and in silico PCR - (reply: 4)
  119. Checking mRNA expression by RT-PCR - (reply: 2)
  120. primer design - over expression (reply: 1)
  121. Bisulfite PCR Question - (reply: 2)
  122. quadraplex RT-PCR error - (reply: 1)
  123. cloning of a fluorecent PCR product - (reply: 1)
  124. NOT SyBrGreenPCR amplification in positive control-Why? - (reply: 1)
  125. how to improve PCR yield? - (reply: 5)
  126. TEST: embed video - PCR song - (reply: 4)
  127. PCR to sequencing problems - (reply: 5)
  128. EXOSAP-IT degrades my PCR products - (reply: 3)
  129. Problems with amplification - (reply: 7)
  130. PCR master mix preparation - (reply: 11)
  131. real time pcr newbie - (reply: 3)
  132. PCR: Big Primers = Big Problems (for me) - I'm really not sure what's going on in those poor little tubes... (reply: 2)
  133. mutations in primer sequence - (reply: 2)
  134. Amount of DNA to run on gel - Be it from PCR or from DNA extraction steps (reply: 4)
  135. Amplification Effeciency - solve the problem with E value (reply: 2)
  136. PCR Inhibition by DNA? - (reply: 5)
  137. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
  138. Total RNA isolation for RT-PCR - (reply: 3)
  139. Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
  140. primer conditions for expression work - (reply: 5)
  141. Failed RT-PCR amplification of 3-&5''-ligated small RNAs - (reply: 5)
  142. Question about primer design targeting GC-rich region - (reply: 2)
  143. how to optimize primer and cDNA concentration for real-time - (reply: 2)
  144. Help -designing primers for real time RT-PCR - (reply: 1)
  145. getting long pcr from cDNA - (reply: 4)
  146. PCR from cDNA library - (reply: 1)
  147. clear culture, but PCR positive - (reply: 4)
  148. Bad primer efficiency during multiplex qPCR - (reply: 1)
  149. phophorylating PCR product after CIP - (reply: 2)
  150. pcr template amount - (reply: 2)
  151. PCR with paraffin-embedded tissue DNA samples - I have had problems to amplify DNA samples from paraffin-embedded tiss (reply: 1)
  152. Problems with amplification curve - (reply: 5)
  153. different transcription from 5' UTR than 3' UTR primers - (reply: 8)
  154. recommended primers for caspase 8 & 9 - human (reply: 4)
  155. Optimising bisulphite PCR primers - (reply: 4)
  156. Strange large bands on my PCR gel, has anyone seen this before? - PCR (reply: 3)
  157. help with RT-PCR - DNase usage - (reply: 2)
  158. PAGE with Realtime PCR product (Sybr Green) - Post-staining with ethidium bromide? (reply: 4)
  159. PCR inhibition in FFPE DNA - (reply: 1)
  160. Determine specific primers and transgene copy number by PCR - (reply: 1)
  161. PCR with Betaine, DMSO, BSA, DTT - (reply: 5)
  162. A couple of (RT-PCR) cowboy questions - I feel the need, the need for speed (reply: 2)
  163. PCR on this gene - products always present? - (reply: 9)
  164. PCR cycle for 80 bases - (reply: 2)
  165. High concentration of antibiotics in amplification of plasmids - concerns about mutation of inserts in plasmid (reply: 1)
  166. my negative PCR control is still positive ! - (reply: 32)
  167. Getting primer dimers in RT-PCR on and off? - (reply: 2)
  168. PCR reaction - quick question! - (reply: 2)
  169. conc of cDNA to be used - PCR to test primers (reply: 5)
  170. optimum conditions for long pcr - (reply: 1)
  171. DIG pcr labeling and northern blot - (reply: 2)
  172. Weekly Decontamination Routine? - To prevent DNA contamination in a PCR lab (reply: 1)
  173. PCR on template with trinucleotide repeats - PCR tips for complexe DNA template (reply: 4)
  174. 1:10 serial dilution in rt-pcr with slope of -0.58 ... - (reply: 1)
  175. Lengthy primers - (reply: 11)
  176. positive colony PCR, but no insert!? - (reply: 5)
  177. Steps in PCR - (reply: 1)
  178. ORF region amplification problem - (reply: 2)
  179. need help on microRNA real-time PCR - (reply: 6)
  180. PCR Help! - Primers-quality checking software (reply: 3)
  181. design primers - (reply: 3)
  182. DNA polymerase that produces blunt end and doesnt smear! - (reply: 2)
  183. PCR on Genomic DNA from cell lysate - (reply: 5)
  184. Primers distinguishing splicing alternatives - (reply: 1)
  185. Problem with bands of PCR products - (reply: 2)
  186. about PCR efficiency - (reply: 2)
  187. TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
  188. primer design from mRNA - (reply: 12)
  189. How do you prevent PCR evaporation? - (reply: 9)
  190. PCR contamination - (reply: 1)
  191. How can I create a T vector for PCR product cloning - (reply: 2)
  192. PCR product won't come down from well - (reply: 3)
  193. RE of PCR Fragment - (reply: 2)
  194. Starting with semi quantitative RT-PCR and a little bit lost.. - (reply: 4)
  195. Storing primers...together? - (reply: 3)
  196. different length for primers amplifying - (reply: 2)
  197. cytosolic PCR control - (reply: 1)
  198. Primer concentration is quantitative Real-time PCR - (reply: 1)
  199. primer dimer annealing temperature - (reply: 2)
  200. What is the function of acetate solution in DNA extraction for PCR used - (reply: 6)
  201. choosing real-time PCR machine - (reply: 13)
  202. Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
  203. some primer dilution calculations - (reply: 7)
  204. PCR - reduced binding efficiency with lower Tm? - (reply: 2)
  205. Purified PCR product - (reply: 2)
  206. Housekeeping genes for RT-PCR in soybean - (reply: 6)
  207. cDNA quantification for PCR - (reply: 5)
  208. no PCR product - (reply: 14)
  209. Can do reverse transcription by gene-specific primers? - (reply: 3)
  210. Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
  211. plz save my PCR product ! - (reply: 4)
  212. primer desighning with RE sites - (reply: 5)
  213. Primer design (long transcripts) - (reply: 2)
  214. Primers for Real time PCR - Really need some advice (reply: 1)
  215. RNA amplification - (reply: 3)
  216. PCR product of unlinearized plasmid - (reply: 1)
  217. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
  218. do you put two pairs primers in one tube when you do site mutations? - (reply: 2)
  219. Will TOPO TA cloning work on "old" purified PCR product? - (reply: 4)
  220. Can PCR on same sample give different results when repeated? - (reply: 2)
  221. RT PCR for methylation analysis - (reply: 3)
  222. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
  223. chip assay primers - chip assay primers (reply: 2)
  224. suitable length DNA for PCR? - (reply: 2)
  225. how to add bases to the primer restriction sites - (reply: 2)
  226. Problems with making mix of PCR reagents ? - (reply: 4)
  227. primer design - (reply: 3)
  228. SYBR green PCR - (reply: 4)
  229. PCR problem, no amplicon, tried everything I could think of.. - (reply: 6)
  230. How to ressuspend primers for Sybr? - (reply: 3)
  231. simple primer calculation - (reply: 5)
  232. What's wrong with my pcr? - high temperture with low specificity (reply: 6)
  233. RT-PCR Vs qRT-PCR help please! - (reply: 4)
  234. 300 bp more after PCR purification - please help - (reply: 3)
  235. primer/probe mix for standard generation? - (reply: 2)
  236. Fast way to get plasmid for PCR - (reply: 2)
  237. PCR efficiency for qRT-PCR analysis - PCR efficiency for qRT-PCR analysis (reply: 4)
  238. PCR failure. GC problem? - (reply: 7)
  239. Help. Trying to sequence my plasmids, but the primers refuse to prime! - (reply: 1)
  240. Degnerate PCR and Sequencing Reactions - (reply: 4)
  241. Forward n reversre primer Tm difference is 10 degree - (reply: 1)
  242. Smearing in PCR - (reply: 4)
  243. Why using heated-top or add mineral oil in PCR - (reply: 2)
  244. primer design (downloadable) programs - not online tools (reply: 4)
  245. BLOOD storage and PCR problems! HELP - (reply: 1)
  246. PCR water contamination - (reply: 5)
  247. figure out the size of amplicon using known primer - (reply: 3)
  248. Primer concentration... - (reply: 5)
  249. How to amplify PCR product - wonder why using linear fragment as template, PCR result was always sm (reply: 1)
  250. Long PCR primers for epitope tagging - where to order? (reply: 3)
  251. Extremely Low Yield PCR with restriction site addition - Assistance requested for beginning molecular biologist on PCR techniqu (reply: 3)
  252. Problems amplifying unstable construct in E.coli - Tips needed (reply: 2)
  253. RT-PCR consistency - (reply: 2)
  254. dried out pcr product - (reply: 1)
  255. Please see the PCR electropherogram - What's wrong with my pcr? (reply: 8)
  256. PCR inhibitors - help ??!! (reply: 8)
  257. QPCR primer Pairs - (reply: 1)
  258. Random Amplification + TA cloning = HATE - (reply: 2)
  259. Quikchange PCR dNTP mix? - (reply: 8)
  260. Inconsistent bisulfite PCR - (reply: 8)
  261. ISH vs. Real Time PCR - correlation between CT-values and quantity - (reply: 2)
  262. no PCR product! any advice? - (reply: 14)
  263. New to rt pcr real time - guidelines and advice (reply: 3)
  264. overlap extension pcr - (reply: 1)
  265. Quikchange mutagenesis primer and PCR design - (reply: 3)
  266. PCR inhibitors in soil DNA - (reply: 6)
  267. How to know tissue specific gene expression by real time PCR - (reply: 3)
  268. Statistics: real-time RT-PCR - (reply: 4)
  269. PCR of AT-rich DNA - (reply: 3)
  270. Primer conc - (reply: 6)
  271. Non-specific Real time PCR signals - (reply: 1)
  272. Does anyone know how to design LUX primers - (reply: 2)
  273. Problems on overlap extenstion PCR - (reply: 2)
  274. Cox-2 primer for PCR - method how to search good cDNA in Pubmed (reply: 1)
  275. ChIP PCR problems - I can't get rid of the smears! - (reply: 6)
  276. PCR primers for CRISPR - primer3 isn't helpful in this case (reply: 1)
  277. QPCR versus regular PCR for Chip - (reply: 2)
  278. Is it necessary to measure the amount of DNA before PCR? - (reply: 3)
  279. miRNA real time PCR - miRNA real time PCR (reply: 2)
  280. DNA amplification for chip-on-chip - WGA or LM-PCR or others? (reply: 12)
  281. PCR primer design, real time versus old school - (reply: 2)
  282. Assembly PCR problem - (reply: 3)
  283. Amplifying cultures - quick question - (reply: 3)
  284. I have a problem with the M13 tailed primer method - M13 tailed primers-SSR (plants) (reply: 1)
  285. PCR Tubes - what kind of lid ? (reply: 6)
  286. What in ChIP samples inhibit PCR? - (reply: 2)
  287. Storage of a plate for real time PCR - Ever stored your already prepared plate for real time PCR? (reply: 7)
  288. specific PCR polymerase - (reply: 3)
  289. help with site-directed mutagenesis - What polymerase and cells to use? (reply: 3)
  290. MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN - (reply: 1)
  291. cloning 708bp PCR prodyct into pET101 vector (TOPO) - (reply: 2)
  292. Primer3 results - (reply: 2)
  293. Problems with several unspecific bands after PCR - (reply: 2)
  294. Colony PCr - (reply: 4)
  295. Cloning PCR product blunt + restriction site - (reply: 2)
  296. cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2)
  297. real time pcr with RNA co-precipitated with glycogen - (reply: 3)
  298. transfect from full plasmid and transfect from PCR product - (reply: 5)
  299. PCR problem..... - (reply: 4)
  300. PCR primers with overhangs - (reply: 2)
  301. How to reduce the dimers - (reply: 3)
  302. magnesium concentration in pcr - (reply: 4)
  303. Primer designing - (reply: 1)
  304. What is your favorite tool for primer design ? - (reply: 2)
  305. Primer express? - Do you use it? (reply: 2)
  306. The best reverse transcriptase for RT PCR - (reply: 5)
  307. Ooops, forgot to add Mg to PCR! - But samples amplified! Can I use them? (reply: 3)
  308. Genotyping - Primers for PCR genotyping (reply: 5)
  309. Enzyme sites on Primer - (reply: 3)
  310. PCR troubleshooting - (reply: 1)
  311. gene-specific Primer for qRT-PCR - (reply: 2)
  312. how to explain this strange amplification chart? - the Ct value comes to be very low because of the unusual amplification (reply: 1)
  313. PCR Troubleshooting - (reply: 2)
  314. No signal in Real-time but in normal PCR?! - (reply: 2)
  315. What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb? (reply: 6)
  316. deletion PCR? - I need help (reply: 1)
  317. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
  318. PCR troubles - PCR works one day but not the next (reply: 10)
  319. PCR from cell culture - to detect the presence of adenovirus (reply: 2)
  320. Wrong PCR product after Bisulfite Treatment-reason? - (reply: 1)
  321. PCR smear with CHIP - (reply: 2)
  322. Smear in PCR after ChIP - (reply: 2)
  323. What happens if different templates dilution in Real-time PCR reaction - (reply: 1)
  324. PCR amplification of region with many ATT repeats - (reply: 1)
  325. PCR primers artifact - (reply: 2)
  326. weak RT-PCR product - (reply: 2)
  327. How many cycles in PCR would you recommend for ChIP - (reply: 3)
  328. real time PCR template - template concentration (reply: 3)
  329. primer design for site-directed mutagenesis - (reply: 3)
  330. what affects primer specificity? - (reply: 2)
  331. modification of PCR buffers - When and how (reply: 1)
  332. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
  333. Dealing with VERY Mg sensitive PCR reactions - (reply: 4)
  334. Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
  335. Can I use different amount of cDNA template to run real time PCR? - when the amount of templates are different,Can I use reference gene to (reply: 2)
  336. Human Epigenome Project primers? - (reply: 2)
  337. Degenerate Primers - Help with Degenerate Primers (reply: 1)
  338. PCR screening of colonies - (reply: 3)
  339. Colony PCR running as smears - (reply: 1)
  340. PCR: Can you get amplification if only one primer anneals? - Same size pcr product using 2 different primer sets (reply: 8)
  341. RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
  342. PCR cloning: neg self-ligation control, many colonies but no insert - (reply: 3)
  343. PCR help! - urgent (reply: 5)
  344. ready to use primers for BSP - (reply: 4)
  345. Troubles in PCR the converted DNA in promoter region - (reply: 6)
  346. Are there any special rules for primer design? - (reply: 5)
  347. ELISA in a real-time PCR plate - possible? (reply: 3)
  348. GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
  349. Unnatural smear in PCR product - (reply: 7)
  350. ChIP PCR help, maybe is the problem of SDS. - (reply: 4)
  351. Colony PCR - (reply: 8)
  352. how to check normal distribution of data - real-time PCR data (reply: 2)
  353. Insert PCR - (reply: 1)
  354. mutant PCR - (reply: 4)
  355. DNA purification technique - After a PCR (reply: 8)
  356. primer design for Syber Green - (reply: 1)
  357. DGGE - PCR and gel conditions - (reply: 2)
  358. PCR machine choice: - (reply: 14)
  359. PCR OF HIV genome - (reply: 1)
  360. Cloning a 60 bp pcr product and Mixing Pfx and Taq polymerases - (reply: 4)
  361. optimizing PCR for low Tm primers - (reply: 1)
  362. Minimal nucleotide for Taq polymerase binding - (reply: 1)
  363. primers TM - (reply: 8)
  364. pcr product degradation - (reply: 1)
  365. Colony PCR - (reply: 11)
  366. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
  367. designing a primer - (reply: 5)
  368. using primers programms - (reply: 9)
  369. qPCR housekeepers - primers or probes? - (reply: 1)
  370. pcr for gene fusion - (reply: 3)
  371. basic question regarding PCR - (reply: 7)
  372. freaky primers - (reply: 5)
  373. How do you design primers of PCR-STR used in Forensic Medicine - Urgent!!!! (reply: 2)
  374. what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
  375. How to get rid of primer dimers - (reply: 2)
  376. problems with PCR primers - (reply: 15)
  377. one nucleotide in the primer is wrong - (reply: 2)
  378. How PCR product becomes gelly? - (reply: 1)
  379. Flag Tagging a PCR product - (reply: 2)
  380. genomic DNA extraction - for PCR (reply: 1)
  381. PCR thermocycler (Eppendorf) - noise problem (reply: 1)
  382. real time pcr in a day - (reply: 5)
  383. PCR efficiency - (reply: 2)
  384. Triton X in PCR - (reply: 7)
  385. real time PCR using two sets of primers with different melt curves - (reply: 4)
  386. AmpliTaq Gold - high fidelity polymerase? - (reply: 2)
  387. Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
  388. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
  389. Introduce restriction sites with PCR primers - (reply: 2)
  390. How much DNA in PCR? - (reply: 6)
  391. Free FastStart Universal Real-Time PCR Master Mixes from Roche - (reply: 7)
  392. primer flaw I'm missing? - (reply: 7)
  393. Blunt end cloning PCR product - (reply: 6)
  394. PCR contamination that comes and goes! - (reply: 5)
  395. How long we can keep Diluted Primer? - (reply: 5)
  396. Real-time PCR....Making sense of it all? - (reply: 3)
  397. PCR primer resuspend method? - (reply: 9)
  398. Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
  399. RT PCR produces nice crisp band but of wrong length :-) - (reply: 2)
  400. Help needed. PCR band problem - (reply: 4)
  401. 2/3Kb PCR fragment - 16S/23S (reply: 3)
  402. Where do dNTP come from? - Chemical or Biological? (reply: 12)
  403. polimorph markers in inbred mouse strains? - microsatellites or any PCR based suggestins are wellcome! (reply: 1)
  404. RT-PCR gene specific amplification problem - (reply: 1)
  405. information regarding PCR using very long primers - (reply: 4)
  406. Non repeatability of RT-PCR with same cDNA - (reply: 3)
  407. MSP primer design - understanding the basics - (reply: 2)
  408. Bisulfite: PCR condition or primer problem? - (reply: 13)
  409. I really need an answer on PCR (16s rRNA), PLS PLS PLS! - (reply: 2)
  410. storage of primers - (reply: 3)
  411. Problem with pcr, help required - PCR product (reply: 12)
  412. Curious - different primer synthesis? - (reply: 4)
  413. Windows in PCR/Clean rooms..... - (reply: 8)
  414. help a student with a PCR process question? - (reply: 6)
  415. too many hits on Blast for primers / microsatellite - (reply: 4)
  416. sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
  417. how to get DNA sequences starting from the primer - (reply: 4)
  418. How to remove a few bases from my cloned insert using PCR - (reply: 3)
  419. PCR help - (reply: 1)
  420. PCR amplification using universal 16s rDNA - it's necessary to do it (reply: 3)
  421. LM-PCR amplification - (reply: 1)
  422. RT-PCR NTC- substitute DNA with water? - or just end up with a smaller rxn volume? (reply: 1)
  423. PCR protocols - for some antibiotic resistant strains (reply: 2)
  424. How to quantitate the pcr product from gel picturess - (reply: 4)
  425. primers for promoter amplification - (reply: 1)
  426. RT-PCR primer designing - (reply: 6)
  427. Scaling up PCR reactions - (reply: 4)
  428. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
  429. cloning through PCR - (reply: 3)
  430. Homemade TaqMan PCR Mastermix? - (reply: 1)
  431. My primers don't work with Chip - (reply: 1)
  432. qPCR using random primers - (reply: 4)
  433. designing primers for cloning - designing primers for cloning (reply: 1)
  434. no bands in PCR reaction analysis - does centrifuge time matter? (reply: 2)
  435. PCR optimization - (reply: 5)
  436. 1. ABI Power SYBR® Green primer efficiency? - (reply: 1)
  437. Polymerase Activity Assay - (reply: 1)
  438. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
  439. PCR inespecific band only in control - why only in the control? (reply: 2)
  440. Identification by 16S rDNA - using short primer (reply: 2)
  441. Degenerate Primers - (reply: 12)
  442. What safety and risk assessments should i be aware of in doing PCR and gel elect - (reply: 2)
  443. restriction enzymes primers - (reply: 5)
  444. Fusion PCR - Help ragrding Fusion PCR (reply: 5)
  445. Roche produce Blunt or dA tail PCR product? - (reply: 3)
  446. Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
  447. Ever heard not of Prime Dimers but Primer Multimers? - (reply: 1)
  448. Isolation of the upstream region of a gene by PCR: a new tecnique? - (reply: 4)
  449. make PCR template blunt ended - (reply: 2)
  450. AT rich PCR problems - anyone experienced with tetramethylammonium chlroide? (reply: 3)
  451. how would you detect a point mutation in the genome? PCR? - (reply: 3)
  452. KOD HiFi DNA Polymerase - (reply: 3)
  453. Need to confirm PCR primers are bad - (reply: 5)
  454. PCR - (reply: 1)
  455. Expectations for presentation of pcr data - (reply: 1)
  456. False negative PCR result - (reply: 5)
  457. help with nested PCR for splice variant detection - nested PCR (reply: 3)
  458. Ligation of half blunt PCR fragments - (reply: 4)
  459. first time doing quantitative real time PCR - (reply: 1)
  460. Help! Primer Dimers… - (reply: 3)
  461. RT-PCR without RNA isolation - (reply: 2)
  462. Very low PCR primer concentrations? - (reply: 4)
  463. Inconsistent PCR results - (reply: 3)
  464. Multiplexing first round PCR in BSP - Any experience? (reply: 16)
  465. PCR detectable units - PDU (reply: 2)
  466. Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
  467. no results in RACE PCR - (reply: 5)
  468. Very resistand band in PCR - (reply: 7)
  469. Knockdown detected by western but not RT-PCR - (reply: 3)
  470. PCR Amplification from clones - (reply: 7)
  471. Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
  472. PCR cloning problem - (reply: 6)
  473. validation of micrarray data by realtime pcr - (reply: 1)
  474. minimum amount of rna in RT-PCR - (reply: 1)
  475. Trying to lyse cells to get PCR template - Need something simpler than a miniprep (reply: 10)
  476. PCR Primer again - (reply: 1)
  477. RAPD PCR - (reply: 1)
  478. Polymerase Choice Troubles - my primer melting temp is so low (~30 C) (reply: 4)
  479. multiplex RT-PCR - (reply: 6)
  480. cycling parameters for PCR - (reply: 4)
  481. primer Tm - (reply: 10)
  482. why no insert but can PCR insert out? - (reply: 5)
  483. smear in degenerate PCR - (reply: 3)
  484. Single Codon Deletion - SOE PCR - (reply: 3)
  485. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
  486. PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
  487. PCR for checking transfection - (reply: 6)
  488. digoxygenin-labelled primer - (reply: 3)
  489. How to import GeBank files into Primer Express 2.0 - (reply: 1)
  490. ChIP: I get smear after PCR? - (reply: 2)
  491. Old PCR publication - help! - (reply: 2)
  492. Primers with RE sites - (reply: 2)
  493. overlap extension using PCR (3way PCR) - (reply: 1)
  494. normalization for real-time PCR - (reply: 4)
  495. what can i do with this pcr? - amplification of cDNA , with primers tm 50 and 67 (reply: 7)
  496. Fusion PCR for eventual TA cloning - (reply: 2)
  497. improve the DNA cons. in RT-PCR - (reply: 2)
  498. Inverse PCR problem! plz help! - (reply: 2)
  499. primer for real-time and reverse transcriptase PCR - (reply: 1)
  500. reverse transcription PCR - (reply: 1)
  501. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
  502. RT-PCR not working - (reply: 8)
  503. PCR for >4kb, i got no product. - how shud i optimize my pcr (reply: 9)
  504. Representing RT-PCR Data - (reply: 2)
  505. do you ever use PAGE to check PCR? - (reply: 3)
  506. PCR puzzle- big or small - PCR (reply: 1)
  507. klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
  508. explaining competitive PCR - (reply: 1)
  509. Anyone ever do a molecular beacon PCR? - (reply: 1)
  510. Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
  511. Primers going bad - what's the best storage/usage conditions? (reply: 4)
  512. 5' Primer modification - Tm-effect ?? (reply: 1)
  513. PCR using an enzyme mix - never done this before, how do I set it up? (reply: 4)
  514. Designing PCR Primers - (reply: 3)
  515. running a gel with primers - (reply: 2)
  516. Long PCR on a cosmid vector - Help needed to run PCR (reply: 3)
  517. the results of allele specific PCR is the other way around - I dont know why (reply: 1)
  518. Will this primer work? - (reply: 8)
  519. More PCR cloning problems! - (reply: 12)
  520. What are the most important parameters in PCR primer design? - Tm difference, GC clamp, GC%, primer-primer complementarity, self-complementarit (reply: 9)
  521. How much PCR do I need for cloning? - (reply: 5)
  522. Primers and enzymes not compatible - How can I fix this? (reply: 2)
  523. cytokines primers - (reply: 1)
  524. How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
  525. PCR cloning problems - (reply: 13)
  526. is there a loss of volume after pcr? - (reply: 5)
  527. Enzyme Site in reverse Primer - (reply: 3)
  528. PCR product self life? - (reply: 2)
  529. general pcr/primer question - (reply: 1)
  530. dephosphorylated vector and PCR insert - (reply: 7)
  531. Need a FRET PCR protocol - (reply: 1)
  532. for the PCR primer what is better the hairpin or the self annealing? - (reply: 9)
  533. blunt end ligation after inverse PCR - (reply: 5)
  534. PCR'ing a high GC gene - (reply: 1)
  535. pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want??? (reply: 5)
  536. PCR problem with CGG repeats - (reply: 4)
  537. different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
  538. Primers for BS treated DNA - (reply: 5)
  539. Restriction digestion of PCR product - (reply: 3)
  540. Problems with PCR for GATEWAY - I'd get rid of 100-150bp unspecific band!!! (reply: 5)
  541. Pfx polymerase for site-directed mutagenesis - (reply: 2)
  542. Nested polymerase reaction - why to do this nested PCR? (reply: 1)
  543. RT-PCR product dilution and electrophoresis - (reply: 2)
  544. forward & reverse primers - (reply: 3)
  545. How to calculate DNA concentration after PCR manually - not using spectrophotometry or electrophoresis (reply: 6)
  546. Taq Polymerase storage conditions - (reply: 1)
  547. pGEM v pTOPO PCR products for sequencing.... Help! - (reply: 3)
  548. role of ammonium sulphate in PCR buffer - (reply: 2)
  549. Primers & probes of Applied Biosystems for real time - (reply: 2)
  550. What is the optimum amount ot bases that can be deleted by PCR? - (reply: 5)
  551. Can I PCR a gene of 2KB? - (reply: 3)
  552. When I chage Taq polymerase - (reply: 2)
  553. Target Amplicon Length for real-time PCR - (reply: 4)
  554. Primer Optimiztion - can this be done in water (reply: 8)
  555. question for the PCR to cloning construct - (reply: 9)
  556. Large fragments from PCR. - problems getting 3.5kb from genomic DNA (reply: 4)
  557. Mycoplasma PCR detection in media/FBS - (reply: 6)
  558. increasing sensitivity and reducing primer dimmer - (reply: 11)
  559. Real-Time PCR Amplicon Question - (reply: 2)
  560. PCR reagents contaminated w/ bact - still ok for fungi? - (reply: 2)
  561. ppt DNA directly from a digestion/ pcr - (reply: 2)
  562. primer design questions for confused - (reply: 2)
  563. Anyone use phusion HF DNA polymerase? - urgent!!!! (reply: 6)
  564. How excess template can inhibit the PCR reaction? - (reply: 1)
  565. Real Time PCR and Gene expression - (reply: 1)
  566. PCR enzyme activity-can you do 50 cycles - (reply: 2)
  567. REAL TIME PCR QUESTION - (reply: 3)
  568. Which primer concentration for RT with very low RNA amounts? - (reply: 1)
  569. Strange problems with B-actin amplification - (reply: 1)
  570. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
  571. restriction site in primers urgent - (reply: 1)
  572. Preparation of template for PCR - (reply: 7)
  573. primer design problems - (reply: 4)
  574. Weird PCR result - (reply: 6)
  575. problems with PCR from chIP material - double band PCR from chIP material (reply: 2)
  576. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
  577. pcr yield and primer concentration - (reply: 5)
  578. PCR Setup stopped working - (reply: 14)
  579. is amplification directly from my ligation reaction possible? - (reply: 1)
  580. Primer design with restriction sites - (reply: 3)
  581. Is a 100bp PCR product more prone to give non-specific signals in a hybridisatio - (reply: 1)
  582. help PCR of GC rich templates - (reply: 3)
  583. I am so disappointed that I failed in PCR again and again - (reply: 4)
  584. differences between oligo and random primers - (reply: 2)
  585. My PCR product is 100bp (approx). What result is more reliable-gel or hybridisat - (reply: 5)
  586. Why is it difficult to PCR taget gene in rice successfully ? - (reply: 1)
  587. PCR contamination - (reply: 2)
  588. Effect of Temperature on PCR - (reply: 3)
  589. Difficulties getting a 4 kb PCR product - (reply: 5)
  590. PCR product multiple bands - (reply: 3)
  591. Any primer design programs...? - (reply: 8)
  592. Which result is correct-Western or RT-PCR - (reply: 5)
  593. Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
  594. PCR problems, huge artifacts, no product - involves 16S primers (reply: 8)
  595. rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
  596. Touch down PCR - (reply: 8)
  597. RealTime PCR of microRNAs - (reply: 9)
  598. Primer design for BSP - (reply: 12)
  599. Designing primers and hybridization probes - (reply: 1)
  600. Junk/dimers on left of single product peak - (reply: 3)
  601. gel purification of PCR product - (reply: 1)
  602. RT PCR Primer - Software (reply: 2)
  603. Possible to clone when you have only 20ng PCR product? - (reply: 10)
  604. Increased signal in IP after peptide addition? - Immunoprecipitation signal amplification (reply: 2)
  605. How to PCR a 100bp fragment - (reply: 4)
  606. Problems with PCR following bisulfite treatment - (reply: 11)
  607. pBluescript cloning of PCR products - (reply: 1)
  608. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
  609. vector PCR problem - stuck in experiments... (reply: 2)
  610. please tell me more about primers with restriction site! - (reply: 4)
  611. Problem in Patch PCR for Site-Directed Mutagenesis - (reply: 5)
  612. transgenic mouse - identification by PCR (reply: 2)
  613. pcr detection of pathogens - (reply: 3)
  614. Recombinant PCR - (reply: 5)
  615. standard curve and differentiation asays - Real Time PCR (reply: 2)
  616. [PCR] Different amplification-efficiency of varying DNA - (reply: 1)
  617. allele-specific amplification - (reply: 1)
  618. Double bands in PCR - WHY? - (reply: 5)
  619. Long range PCR - What enzymes do you use? - (reply: 6)
  620. sear in PCR no bands - (reply: 3)
  621. Few colonies with cloning of PCR product after digestion - (reply: 7)
  622. can primers age? - (reply: 1)
  623. No PCR Product after Bisulfite Modification - (reply: 5)
  624. PCR with 75 mer forward primer - (reply: 4)
  625. half life of primers - (reply: 2)
  626. competition of multiple PCR products - (reply: 1)
  627. PCR from Genomic DNA - (reply: 3)
  628. about the primers in MSP - (reply: 5)
  629. ARMS PCR - (reply: 3)
  630. cloning problem with pcr and pegfp vector - (reply: 9)
  631. What annealing temperature is better for my primers? - (reply: 7)
  632. primer concentration (calculation problem) - (reply: 5)
  633. Problem with cloning p53-suggestions for RT-PCR - (reply: 1)
  634. PCR: same premix, same everything, different results... - (reply: 21)
  635. How to confirm a 80bp PCr fragment? - (reply: 5)
  636. RT-PCR control for Vitis vinifera - (reply: 2)
  637. Problem PCR amplifying gene promoter regions - (reply: 4)
  638. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
  639. primer:cross-dimer - How to remove the cross dimer? - (reply: 3)
  640. PCR trouble.....help! - (reply: 16)
  641. My PCR's are killing me! - nothing is working!! (reply: 9)
  642. PCR almost like false positive problem! - How can I get rid of 5bp band near mine !!!!!!! (reply: 2)
  643. dNTP dilution for PCR - (reply: 4)
  644. can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
  645. alternative to dmso in pcr - (reply: 3)
  646. The weirdest primer ever - (reply: 7)
  647. Trouble with real-time PCR standard curve - (reply: 4)
  648. no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
  649. What happens to PRIMERS at 72 C ? - (reply: 5)
  650. PCR cleanup - Quick question (reply: 1)
  651. No PCR product detected in agraose gel - (reply: 7)
  652. 2 melting peaks in real-time PCR - (reply: 1)
  653. visualizing unknown DNA with linkers and PCR - (reply: 2)
  654. DNA fingerprinting - Is it necessary to use DNA polymerase with proof-read function in PCR? (reply: 2)
  655. checking colonies by vector primers.... - (reply: 7)
  656. Does 2-mercaptoethanol inhibit PCR reactions? - anyone know? (reply: 7)
  657. Nested PCR troubleshooting - no secondary product - (reply: 5)
  658. adding restriction sites to degenerate primers - (reply: 9)
  659. Two to use PCR to get domain deletion within a protein? - (reply: 2)
  660. Standard vs High fidelity polymerase, please help - No results with high fidelity (reply: 5)
  661. PCR on a GC rich region - PCR on a GC rich region (reply: 4)
  662. PCR amplification of Community DNA - (reply: 6)
  663. Unwanted band seen along with my pcr product. - regarding PCR product with unwanted band formation during electrophoresis (reply: 3)
  664. cloning primer and tag questions - first try (reply: 2)
  665. PCR problems with 1,7kb fragment - (reply: 8)
  666. reuse PCR gel - (reply: 2)
  667. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
  668. Two design programs give very different primer Tm values - RT-PCR (reply: 5)
  669. Problem of Smear - Smearing of PCR product (reply: 7)
  670. cDNA synthesis primer - (reply: 3)
  671. gsamsa - Gene array vs PCR (reply: 4)
  672. Any one can tell me what happens to my RT-PCR? - Urgent !RT-PCR really kills me! (reply: 5)
  673. PCR of herbarium specimes - PCR of Caesalpinia herbarium specimes (reply: 1)
  674. pcr product transfection - (reply: 8)
  675. restriction digestion of PCR product? - (reply: 10)
  676. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
  677. Taq polymerase vs HotstartTaq polymerase - (reply: 4)
  678. relative quantification, primer pair problem - (reply: 1)
  679. Question about PCR reaction condition - (reply: 3)
  680. PCR Internal Controls - (reply: 2)
  681. smear in PCR - No consistancy in amplification (reply: 3)
  682. Designing species specific primers for E.coli - please help? (reply: 3)
  683. Chloramphenicol amplification - (reply: 5)
  684. Screening a Gene Library - I want to use colony PCR, but How (reply: 4)
  685. Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
  686. Need for Nested PCR - Primers Designed with MethPrimer - (reply: 6)
  687. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
  688. 2nd round of amplification issues - (reply: 1)
  689. Smearing of the PCR Products - (reply: 10)
  690. Stick T7 on custom primer for sequencing? - (reply: 2)
  691. SMEAR IN POSITIVE CONTROL IN LONG PCR - (reply: 3)
  692. Cloning PCR product - Any ideas? (reply: 3)
  693. MSP primer design help - (reply: 5)
  694. Primers for nested-PCR - (reply: 1)
  695. no product after PCR - (reply: 5)
  696. Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
  697. making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
  698. BSP and MSP Primers - (reply: 2)
  699. PCR question, need help please! - (reply: 5)
  700. Bisulfite sequencing primer - (reply: 4)
  701. specific loci not present depending on DNA extraction method? - DNA extraction and succesful PCR (reply: 1)
  702. Methods for ligating fragments to form dimers.. - (reply: 1)
  703. Must all BSP be nested PCR? - (reply: 4)
  704. QPCR, where to start? - optimizing primer and template (reply: 5)
  705. about nested PCR - (reply: 2)
  706. Primer for sequencing - need help!!!!!!!!!! (reply: 6)
  707. Primer design invert pcr - (reply: 5)
  708. Standard PCR - (reply: 3)
  709. Left my PCR supplies out overnight. Are they all ruined? - (reply: 4)
  710. faster way to identify RNA virus other than RT-PCR? - (reply: 2)
  711. Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
  712. designing primers for Real Time - (reply: 1)
  713. Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
  714. Real time PCR late amplification - (reply: 6)
  715. PCR anealing temperature question - (reply: 12)
  716. Taq and Pfu polymerase give different amplification - (reply: 8)
  717. Methods for primer reconstitution - (reply: 23)
  718. Help on PCR purification - (reply: 2)
  719. BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
  720. Troubleshooting PCR contamination - (reply: 13)
  721. no result with pcr. is it the primer?/ - no amplification in pcr even after starting from the beginning (reply: 6)
  722. PCR result: Nothing! - Urgent! (reply: 2)
  723. PCR large fragments - (reply: 2)
  724. can we add the terminaton codon in the 3' primer? - (reply: 1)
  725. PCR probes for Southern blot - (reply: 1)
  726. Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
  727. designing primer for methylation - (reply: 21)
  728. Primer dimers?Contaminations? - Unexpected bands! (reply: 5)
  729. Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
  730. Use PCR products to determine efficiency of amplification? - (reply: 7)
  731. Band in negative PCR control - (reply: 25)
  732. question in 5' RACE and primer extesion - (reply: 8)
  733. Primer concentration ? - (reply: 2)
  734. PCR my cDNA & got nothing - (reply: 2)
  735. PCR product dimer (not primer dimer!) - PCR (reply: 2)
  736. Degradation of PCR product during purfication - Purification of PCR product for cloning (reply: 2)
  737. Nonspecific PCR amplification? - (reply: 2)
  738. Plasmid PCR - (reply: 5)
  739. Nested allele-specific PCR - (reply: 1)
  740. problems in subcloning PCR products for TA cloning - (reply: 4)
  741. no product for promoter genomic PCR - (reply: 3)
  742. from which strand to derive BSP primers? - (reply: 5)
  743. SMear instead of PCR product - (reply: 3)
  744. Best product size for real-time RT-PCR - (reply: 4)
  745. what is the principle of gradient PCR? - How does it work (reply: 2)
  746. Why is my smaller band too faint? Genomic PCR not working! - Just trying to genotype some mice... (reply: 2)
  747. Removal of PCR inhibitor - (reply: 3)
  748. Both the PCR primers in a vial. - Did anybody mix the diluted primers in single tube for use? (reply: 7)
  749. Concentrating PCR product - (reply: 9)
  750. How to design primer for sequencing unknown DNA - (reply: 5)
  751. PCR mix and DEPC water - (reply: 2)
  752. need helps on probe design--human ip10 primers and probe. - (reply: 2)
  753. PCR - (reply: 8)
  754. Success with Direct Sequening of Bisulfite Pcr Products - (reply: 47)
  755. how to determin the Annealing Temp of ristriction sites modified primer? - help me ! (reply: 26)
  756. two bands of one gene in PCR - (reply: 8)
  757. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
  758. About PCR primers' concentration - (reply: 2)
  759. Multiple unknown amplification products - (reply: 2)
  760. Qiagen Gel Extraction of PCR product - (reply: 7)
  761. Problem with lids to 8-strip .2 ml PCR tubes - (reply: 3)
  762. Measuring the amount of PCR product - (reply: 9)
  763. difficult PCR - (reply: 1)
  764. Low PCR product for antibody gene - (reply: 4)
  765. Colony PCR positive, miniprep negative? - (reply: 9)
  766. RT-PCR machine/setup problems - (reply: 2)
  767. Primer dimers - (reply: 1)
  768. troubles with human genomic DNA amplification - (reply: 1)
  769. RT-PCR question - genomic DNA amplified with RNA (reply: 9)
  770. Preserve PCR mix - (reply: 3)
  771. problem concerning PCR Test after disruption and yeastgenome DB - (reply: 6)
  772. primers design for mutagenesis of two nearby sites - mutations (reply: 2)
  773. Asymmetric PCR - (reply: 2)
  774. PCR of long fragment(approx 8 kb) - (reply: 3)
  775. PCR..HELP - band in no template control (reply: 1)
  776. Troubleshooting PCR smear - Urgent,Need help! (reply: 4)
  777. [urgent help] Band missing in PCR - (reply: 5)
  778. Multiplex PCR - (reply: 4)
  779. Smearing in nested pcr product - (reply: 2)
  780. Intensive primer dimers - (reply: 2)
  781. PCR using different primers to generate same DNA sequence - explanation please :) (reply: 2)
  782. PCR no longer works - no pcr product? (reply: 3)
  783. What should I do for more samples need to be tested by real-time PCR? - (reply: 1)
  784. Laddering effect in PCR blank control - (reply: 4)
  785. Do you synthsize your own oligo(dT) primer? - (reply: 2)
  786. PCR on Genomic DNA - (reply: 2)
  787. Could higher ammount of taq be inhibitory for overall PCR reaction? - (reply: 2)
  788. genotyping mice-no pcr product - (reply: 3)
  789. Nested PCR - no amplicon - (reply: 1)
  790. PCR - (reply: 3)
  791. Volume of Real time PCR mastermix and reaction - (reply: 4)
  792. Weird duplex PCR results - (reply: 5)
  793. PCR - (reply: 4)
  794. potassium acetate in primer solution - I made a mess but need to recover (reply: 6)
  795. cloning/amplifying attenuate RNA transcripts - (reply: 6)
  796. Nested PCR problems - (reply: 1)
  797. confirmation of my pcr product - (reply: 2)
  798. 16S rRNA primers for E. coli - (reply: 2)
  799. About PCR smear - Anyone can help me? (reply: 4)
  800. primer design - how to design primers (reply: 2)
  801. Real-time PCR problems - RT_PCR (reply: 7)
  802. HIV RT PCR KIT - (reply: 1)
  803. Real time PCR - no product - (reply: 1)
  804. Multiplex Primers - Primer primer interaction (reply: 1)
  805. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
  806. PCR smear in samples and negative control - (reply: 5)
  807. How should I check my colonies? - PCR check is not working...:( (reply: 5)
  808. question about mutagenic PCR. - (reply: 2)
  809. phenol/chloroform of purified PCR product after RE digestion - what are we trying to purify? (reply: 4)
  810. PCR and topo problem - (reply: 3)
  811. Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers?&# - (reply: 6)
  812. How to calculate absolute fold change in expression by realtime PCR - Realtime PCR (reply: 2)
  813. Amplification of 3KB protein from Hela mRNA PCR PROBLEM - PCR Amplification of 3KB protein (reply: 6)
  814. qPCR for HKG and traditional PCR for GOI - (reply: 1)
  815. Smeary bands in real-time RT-PCR - (reply: 7)
  816. RT PCR - (reply: 1)
  817. How to purify PCR product? - (reply: 3)
  818. PCR internal control - how does it work? (reply: 1)
  819. False positive for PCR screening of insert - (reply: 1)
  820. why using Vent-exo polymerase? - (reply: 2)
  821. Programs for designing Primers - (reply: 6)
  822. Disadvantages of using oligo-dT primers - (reply: 1)
  823. Selective amplification problem in AFLP - (reply: 1)
  824. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
  825. Do I need internal controls in MS-PCR? - Methylation specific PCR Internal controls (reply: 3)
  826. PCR Trouble shooting - PCR Trouble shooting (reply: 2)
  827. ES cell genomic DNA as PCR template for screening - (reply: 5)
  828. Problem with PCR amplification - (reply: 2)
  829. Simple PCR - (reply: 1)
  830. targetted pcr - targetted pcr (reply: 3)
  831. Sequencing primer dilution - (reply: 2)
  832. PCR for cloning - PCR (reply: 2)
  833. PCR with degenerate primer - (reply: 6)
  834. 1.3kb PCR only shows primer dimer - (reply: 2)
  835. Titration of Mg2+ in RT-PCR - (reply: 3)
  836. RT-PCR primers for E. coli LacZ gene - (reply: 3)
  837. SOS: real time PCR standard curve - (reply: 1)
  838. Serratia PCR - (reply: 6)
  839. QuickChange mutagenesis: how to do it without kit? - What dNTP concentration should I use? (reply: 1)
  840. Long distance (16 kb) PCR problem - (reply: 12)
  841. Primer for 4 genes sharing short consensus sequence - (reply: 2)
  842. Looking for online primer design for SNaPshot - (reply: 1)
  843. ask for an explanation to pcr ladder at high cycle number - help (reply: 2)
  844. PCR primers: hairpin and dimers? - what can I do to avoid this? (reply: 4)
  845. BSP - favors amplification of unmethylated template? - (reply: 2)
  846. PCR on PCR of cDNA smear - (reply: 1)
  847. Reconstitute primers - (reply: 4)
  848. PCR product - (reply: 5)
  849. Delta-delta Ct method and PCR efficiencies - (reply: 9)
  850. multiplex PCR for three sets of primers - (reply: 1)
  851. repeat PCR amplification problem - (reply: 2)
  852. to purify these PCR products or not? - preparation for restriction digest (reply: 5)
  853. How many mismatches in point mutation primers? - (reply: 3)
  854. Software available for Multiplex PCR primer designing - (reply: 3)
  855. PCR of ligation products - (reply: 8)
  856. multiplex pcr trouble - some bands won't amplify (reply: 16)
  857. Subcloing PCR fragments - (reply: 1)
  858. 3' - 5' activity of Taq DNA Polymerase - (reply: 2)
  859. Amplify 4.5 kb pcr product without using special polymerase - (reply: 10)
  860. SOS! asymmetric PCR, no PCR product - (reply: 2)
  861. RT-PCR- help - Polyacrylmide Gel electrophrosis for PCR products (reply: 3)
  862. Mouse MSP primers - (reply: 11)
  863. Primer Dimer problems in real-time PCR - (reply: 14)
  864. primer orientation - (reply: 1)
  865. Degenerate primers for nested PCR? - (reply: 1)
  866. Looking for real time PCR workshops - real time PCR workshops (reply: 2)
  867. Reliable ChIP primers for Sp1? - (reply: 2)
  868. increased primer dimer with template - (reply: 2)
  869. How long should the DNA fragment be amplified by the two-step PCR? - (reply: 8)
  870. primer efficiency in real time PCR - need for a method explanasion (reply: 1)
  871. PCR High GC content - PCR High GC content (reply: 11)
  872. PCR, contamination or not - (reply: 1)
  873. design of PRC primers of full ORF - (reply: 3)
  874. Two round MSP with the same primer - (reply: 1)
  875. faint pcr bands to some, intense to some - (reply: 5)
  876. Designing a Primer within an exon - Consesus required - (reply: 2)
  877. Contamination/no amplification in MSAP-PCR - (reply: 3)
  878. No bands at all in gel for PCR products - (reply: 5)
  879. Finding PCR Limiting Reagent - (reply: 5)
  880. problem wiht long pcr fragment - (reply: 2)
  881. difference between primer extension and RACE - (reply: 6)
  882. Is RealTime PCR data enough for Telomerase activity? - (reply: 4)
  883. No insert for cloning PCR product into expression vector - (reply: 5)
  884. designing QRT-PCR Primers - (reply: 11)
  885. multiplex PCR - (reply: 4)
  886. How can I get the 4.5kb Human typeI procollagen alpha1 chain cDNA Via RT-PCR fr - Seeking Help (reply: 14)
  887. PCR Smear - (reply: 2)
  888. More RT PCR questions: - can I amplify "everything?" (reply: 2)
  889. Ethidium Bromide vs Sybr Green stain (normal PCR) - (reply: 5)
  890. PCR master Mix problem...Plz help me out - PCR (reply: 8)
  891. RT-PCR on plasmid - (reply: 1)
  892. Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
  893. Detection of Bt gene in Corn Through PCR - High School Science Fair - (reply: 6)
  894. separation of pcr products of similar sizes in agarose gel - (reply: 1)
  895. Can I add additional sodium ion to PCR reaction buffers? - (reply: 5)
  896. PCR product precipitation - (reply: 4)
  897. Cloning PCR product - double bands or heterozygote (reply: 5)
  898. virtual PCR - (reply: 3)
  899. Real-time PCR machines for DNA quantitation - (reply: 5)
  900. purification by gel? - after a first round of PCR (reply: 2)
  901. Does Reverse Transcription have to be done using PCR - (reply: 6)
  902. how to improve the efficiency of my FQ-PCR - I can not get a beautiful amplification curve (reply: 3)
  903. PCR of AmpR gene - PCR of Amp gene (reply: 1)
  904. PCR reactions - What condition should I use? (reply: 5)
  905. inverse PCR or promoter trapping - (reply: 2)
  906. Taq polymerase - (reply: 5)
  907. Methylation & PCR - (reply: 1)
  908. TaqMan probes, pcr mix - (reply: 1)
  909. What way would you suggest to clean PCR products? - (reply: 4)
  910. Primer design question - Use of primer 3 (reply: 3)
  911. Long PCR for amplification from plasmid - (reply: 5)
  912. PCR after Sonication... - (reply: 10)
  913. PCR trouble - (reply: 16)
  914. cloning of PCR product - (reply: 1)
  915. KOD plus for PCR - (reply: 1)
  916. RT-PCR Primer design - which region? - (reply: 2)
  917. Lower size band in genomic PCR with 300 bp missing - (reply: 12)
  918. PCR amplicon size and contamination - (reply: 4)
  919. Gelatin for PCR - (reply: 2)
  920. Question about gel purification of PCR product - (reply: 1)
  921. nested PCR - (reply: 3)
  922. No PCR products in MSP with MethPrimer designed primers - (reply: 2)
  923. Taq polymerase dilution? - (reply: 3)
  924. help pcr cloning problem - help pcr cloning problem!!!!!1 (reply: 2)
  925. problems with mix of MSP's primers - (reply: 2)
  926. [PCR products] - (reply: 1)
  927. real time PCR with sybr green - having problems validating my knock down (reply: 3)
  928. PCR an insert in a vector with wrong product size - (reply: 1)
  929. RNA extraction with TRizol and RT-PCR - (reply: 8)
  930. design primer for PCR.. - (reply: 3)
  931. Amplitaq Gold pcr master mix - Protocol question (reply: 1)
  932. realtime pcr validating the results of microarray? - (reply: 7)
  933. gel extraction of pcr products - (reply: 3)
  934. PCR for more than 5 kb - (reply: 4)
  935. MSP primers not working - (reply: 1)
  936. Quick cloning question - Purification of PCR fragment (reply: 1)
  937. problems with bisulfite PCR - (reply: 8)
  938. extra sequence in primers - (reply: 4)
  939. Amplification problems - (reply: 9)
  940. Sybr RT PCR problem - (reply: 4)
  941. calculating Tm of primers - (reply: 8)
  942. AmpliTaq Gold instead of TaqMan Universal PCR Mix in Real-time? - (reply: 3)
  943. RT-PCR for large fragment - (reply: 7)
  944. nested PCR after bisulphite - (reply: 3)
  945. design pcr primers - (reply: 3)
  946. RT-PCR problem - negative control (reply: 1)
  947. Random genoic amplification for microarray - (reply: 4)
  948. Real-time PCR - contamination or not? - (reply: 6)
  949. Degenerate PCR troubleshooting - (reply: 3)
  950. A problem of quantitative PCR - (reply: 2)
  951. maximum size for PCR - (reply: 3)
  952. Bisulfite treated DNA specific primer set critique? - (reply: 18)
  953. 60 bp PCR and primer dimers - (reply: 6)
  954. faint bands of PCR products - (reply: 5)
  955. PCR contamination with human DNA - (reply: 5)
  956. bisulfite modification after pcr - (reply: 2)
  957. Degenerate PCR troubleshooting - (reply: 9)
  958. effect of ethanol and dna concentration in pcr - (reply: 3)
  959. PCR small framents - (reply: 2)
  960. real time PCR (inconsistant results) - (reply: 2)
  961. PCR optimization - (reply: 12)
  962. no band obtain after PCR - (reply: 2)
  963. Problem with PCR - (reply: 4)
  964. primers for SoE - (reply: 1)
  965. Trouble with Inverse PCR - (reply: 8)
  966. multiple band on PCR - (reply: 5)
  967. PCR enhancer - (reply: 5)
  968. Discussion: ChIP PCR control - (reply: 12)
  969. designing flanking primers around msp primers - (reply: 1)
  970. Help: (PCR) Increase template, but NO Increase in product - (reply: 5)
  971. PCR purification basics? - (reply: 6)
  972. No PCR products with the ChIP DNA - (reply: 5)
  973. primer does not bind - help (reply: 2)
  974. reconstitute primer - (reply: 4)
  975. RT-PCR scale-down makes more sensitive? - (reply: 3)
  976. Purification of short PCR product - (reply: 4)
  977. PCR Supermix vs Roche - (reply: 3)
  978. SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
  979. PCR contamination by recombination - (reply: 1)
  980. RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
  981. T4 DNA poymerase - T4 DNA polymerase (reply: 5)
  982. Unmethylated primer control - (reply: 4)
  983. Cloning large pcr product - efficiency (reply: 2)
  984. how much total RNA do you use for RT-PCR - (reply: 6)
  985. Extremely PCR scale down - (reply: 4)
  986. mastermix for Real-time PCR - (reply: 2)
  987. PCR from genomic DNA - (reply: 1)
  988. PCR on Leishmania DNA - (reply: 1)
  989. Buffers for storing and diluting of dNTP - (reply: 3)
  990. Help a first time cloner with PCR! - First time cloning (reply: 5)
  991. Primers for RT reaction - (reply: 6)
  992. Real Time PCR - Relative Quantitation - Choice of calibrator and calculations (reply: 1)
  993. PCR product size with a Roche Lightcycler? - (reply: 1)
  994. Primers design: how to check for mispriming? - (reply: 2)
  995. PCR from genomic DNA - (reply: 6)
  996. standard curves - real time pcr (reply: 3)
  997. EDTA - real time PCR (reply: 3)
  998. Pls check my bisulphite sequencing primers - (reply: 12)
  999. Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
  1000. How to setup control for bisulfite sequencing PCR - (reply: 2)
  1001. Bad Primer - (reply: 2)
  1002. PCR problems - (reply: 3)
  1003. delta delta Ct method - Real time PCR (reply: 4)
  1004. Reverse-transcriptase PCR troubleshoot HELP - (reply: 13)
  1005. PCR troubleshooting - Primers (reply: 3)
  1006. Calculating correct melting temperature for primers - (reply: 2)
  1007. How can I add sequences to DNA using PCR? - (reply: 1)
  1008. end of primer - (reply: 2)
  1009. T/A cloning of cDNA? - PGEM-T vs pCR 2.1 T/A TOPO? (reply: 3)
  1010. PCR primer dimer? - (reply: 7)
  1011. How many times for a run -real time pcr - (reply: 1)
  1012. How to avoid secondary structure in primer - (reply: 2)
  1013. subcloning of PCR product, please help! - subcloning problems (reply: 12)
  1014. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
  1015. real-time PCR - (reply: 5)
  1016. Realtime PCR standard curves - (reply: 8)
  1017. Real time PCR supermix - (reply: 4)
  1018. PCR contamination mystery - (reply: 2)
  1019. Real-time PCR amplification curve up and down - (reply: 3)
  1020. Which RT primer is better for qPCR - (reply: 2)
  1021. HEPA filter - and PCR contamination (reply: 1)
  1022. Problem with amplifying DNA from FTA cards - Is there a special trick involved? (reply: 1)
  1023. Why different amounts of forward and reverese primers in RT-QPCR - (reply: 2)
  1024. primer optimization - (reply: 1)
  1025. PCR on plasmids? - (reply: 2)
  1026. Pfx PCR problems - (reply: 1)
  1027. Storage of RT-PCR products - (reply: 2)
  1028. PCR of a large DNA fragment - (reply: 3)
  1029. Pipetting on ice - during PCR (reply: 5)
  1030. Sometimes i get bands, sometimes i get nothing for my PCR - Strange PCR Results (reply: 1)
  1031. Multiplex PCR troubleshooting - (reply: 6)
  1032. Real Time qPCR - qPCR SYBR greenI primer design (reply: 5)
  1033. Primers for species identification - Know any good? (reply: 6)
  1034. Genotype ratio with quantitative PCR - (reply: 1)
  1035. can't get rid of DNA contamination even with DNase for RT-PCR - (reply: 3)
  1036. PCR master mix or super mix - PCR technique (reply: 1)
  1037. PCR screeing for positive transormation - (reply: 1)
  1038. Contamination in PCR cloning - (reply: 3)
  1039. PCR fragment analysis - please help! - PCR fragment analysis - please help! (reply: 2)
  1040. Hot start PCR - (reply: 6)
  1041. mutilple bands after RT-PCR - (reply: 2)
  1042. How to measure the concentration of primers? - (reply: 2)
  1043. Probes or primers that span introns - (reply: 6)
  1044. Problem with second PCR - 1st PCR product and 2nd PCR product are different (reply: 1)
  1045. Cloning a large PCR product - trouble cloning large DNA (reply: 3)
  1046. real-time pcr plasmid standards - (reply: 4)
  1047. How much of the starting amounts for Real time PCR? - (reply: 1)
  1048. PCR parameters - (reply: 2)
  1049. Unequal amplification efficiency of heat blocks - (reply: 4)
  1050. PCR colony screening - (reply: 9)
  1051. help regarding primers reconstitution - (reply: 4)
  1052. PCR - Long PCR with short primers (reply: 1)
  1053. Calculating Tm of a PCR product? - (reply: 6)
  1054. Sequencing of PCR products - (reply: 5)
  1055. a Takara TA taq DNA polymerase protocol - (reply: 1)
  1056. how long a primer should I use? - (reply: 1)
  1057. problem with PCR --using fragment as template - (reply: 12)
  1058. real time PCR kit components - (reply: 2)
  1059. identifying exon and intron for primer design - (reply: 2)
  1060. PCR Genomic DNA - PCR Genomic DNA (reply: 1)
  1061. PCR question - (reply: 3)
  1062. Real Time PCR and melting curve - (reply: 2)
  1063. threshold for real time PCR - (reply: 1)
  1064. TA cloning with short PCR products - use of TA cloning kit (reply: 4)
  1065. How do you prepare PCR stock solution to minimize pipetting - (reply: 37)
  1066. multiplex PCR for 9 exons - (reply: 5)
  1067. Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system? (reply: 1)
  1068. tRNA and Real time RT-PCR - (reply: 1)
  1069. Designing methylation primers on long CpG island - (reply: 14)
  1070. 3 bands on RT-PCR - (reply: 4)
  1071. DNaseI have affect with RT-PCR or not - (reply: 3)
  1072. RT- PCR? - (reply: 2)
  1073. real time PCR primer for methylation - (reply: 2)
  1074. Determine the RNA concentraiton for linear PCR - (reply: 1)
  1075. contamination in real-time pcr negative control - (reply: 12)
  1076. TOPO TA pCR 2.1 cloning - (reply: 1)
  1077. Real-time PCR problems - (reply: 3)
  1078. software to find restriction site and TM for primer - (reply: 7)
  1079. Large scale PCR to get milligram of DNA - (reply: 6)
  1080. real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
  1081. PCR problem: DISTINCT bands far shorter than target! - (reply: 3)
  1082. genotyping transgenic mice with PCR - (reply: 1)
  1083. Failed to amplify a 2.1 kb PCR product - (reply: 3)
  1084. About PfuTurbo® DNA Polymerase and Pfu DNA Polymerase - (reply: 1)
  1085. realtime RT-PCR/internal control - (reply: 3)
  1086. PCR LABORATORY - to avoid contaminations... (reply: 1)
  1087. PCR contamination - (reply: 1)
  1088. PCR low amplification - (reply: 2)
  1089. RT-PCR gone bad - (reply: 4)
  1090. Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
  1091. Which vector for cloning big PCR fragments? - (reply: 3)
  1092. microsatellite PCR from poor quality/trace amounts of DNA - (reply: 3)
  1093. Asymmetric PCR for generation of single stranded DNA - (reply: 5)
  1094. GC content of PCR primers - (reply: 3)
  1095. influence of MgCl2 in PCR - (reply: 7)
  1096. RT-PCR - (reply: 1)
  1097. cpgware primer design, does it work - (reply: 4)
  1098. RT-PCR - 2 kb fragment in RT-PCR (reply: 2)
  1099. Who will give me a software to design primer for PCR? - (reply: 3)
  1100. No PCR Product for Sequencing - (reply: 5)
  1101. PCR - No bands anymore in well functioning PCR - (reply: 6)
  1102. problems with PCR long target gene - (reply: 2)
  1103. No PCR Product - (reply: 2)
  1104. PCR comparison of gene expression - (reply: 5)
  1105. "PCR ghosts" - :wacko: (reply: 2)
  1106. Sequence input for bisulfite PCR primer design - (reply: 1)
  1107. Gradient PCR - (reply: 2)
  1108. PCR Failure..pls help! - (reply: 3)
  1109. Real Time PCR Annealing vs. Melting - Relation of anneal temp rxn to melt temp primer (reply: 2)
  1110. Is there a PCR length limitation after Bisulfite treatment? - (reply: 3)
  1111. RT-PCR with B-cells as template - (reply: 1)
  1112. PCR for ChIP - PCR for ChIP not working (reply: 3)
  1113. primer design for bisulphite treated DNA - (reply: 1)
  1114. PCR more difficult in methylated CpG region? - (reply: 7)
  1115. PCR of unknown DNA - PCRing unknown DNA with known linkers (reply: 2)
  1116. Quantification of PCR using delta-delta Ct method. - Quantification (reply: 1)
  1117. ligation of pcr product - (reply: 4)
  1118. TAQ polymerase is needed - TAQ polymerase for 60 cycles (reply: 1)
  1119. How to determine PCR annealing temperature - (reply: 9)
  1120. sometimes I get it sometimes I dont - My PCr does not work always (reply: 9)
  1121. allele-specific PCR - detection of SNPs by allele-specific amplification (reply: 4)
  1122. How to remove PCR inhibitors from DNA - (reply: 7)
  1123. Problem cloning PCR product into double-cut vector - (reply: 3)
  1124. RT-PCR primer design - exon-exon primer design (reply: 5)
  1125. Primer design - PCR for cloning (reply: 6)
  1126. Promoter amplification.... - (reply: 7)
  1127. 1-2kb PCR products TOPO cloning troubles - help (reply: 4)
  1128. RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (reply: 1)
  1129. PCR product for ligation - no PCR product from PCR product (reply: 1)
  1130. availabre primers for MSP - (reply: 1)
  1131. Strange mobility behavior of PCR products on agarose gel - EB stained vs Gelstar stained (reply: 2)
  1132. PCR or RT-PCR? - (reply: 3)
  1133. 3' end of forward primer mismatch - (reply: 5)
  1134. Problem with PCR on difficult template - How to amplify GC-rich region? (reply: 6)
  1135. DNA quantification - How to quantify my PCR product before sequencing?? (reply: 8)
  1136. RACE - specific primers in first strand synthesis (reply: 4)
  1137. cloning PCR fragment - PCR fragment is not getting cloned in the vector (reply: 2)
  1138. checking for inserts with colony PCR - (reply: 6)
  1139. Primer Designer Online - Pipleline use of designer (reply: 1)
  1140. PCR-Check, Screen-Check, MiniPrep - HUH?? - PCR product transformed, grown on AMP, no mini (reply: 2)
  1141. Cloning a long PCR product - (reply: 2)
  1142. If the primer is dimer,it's ok? - (reply: 5)
  1143. Troubleshoot PCR smear problem - all I got was smear!! (reply: 71)
  1144. cloning size is not correct - 2 bands on the PCR product (reply: 2)
  1145. Multiplex PCR primer designing - (reply: 4)
  1146. still more about cloning PCR products - (reply: 9)
  1147. Annealing temperature for PCR - (reply: 3)
  1148. Genomic DNA amplification - (reply: 4)
  1149. RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
  1150. PCR is6110 Tuberculosis - Non specific amplification (reply: 2)
  1151. Bisulfite modification kits and PCR - (reply: 5)
  1152. Transformation and PCR questions - (reply: 2)
  1153. PCR contamination in negative control - (reply: 3)
  1154. PCR Cloning - primer design and CpG methylation (reply: 4)
  1155. cloning from PCR - (reply: 25)
  1156. sowing pcr? - (reply: 5)
  1157. How to identify plasmid - using enzyme and PCR or other? (reply: 2)
  1158. BiPolar Clamps on PCR primers for TGGE - (reply: 1)
  1159. multiplex primers - (reply: 6)
  1160. Bisulfite PCR: Smear - Can't amplify a certain sequence (reply: 4)
  1161. PCR product sequencing BD v3.1 - Help in removal of residues (reply: 1)
  1162. RNA and DNA extraction and PCR control - (reply: 1)
  1163. About Q-PCR(real-time PCR) - COMPARE (reply: 1)
  1164. dissociation curve of real time pcr products - 2 different peaks in dissociation curve (reply: 2)
  1165. parse primer3 output - (reply: 3)
  1166. bisulfite pcr primers - (reply: 1)
  1167. The PCR Suite - a set of new primer design tools - (reply: 1)
  1168. pcr ligation - problems with ligation (reply: 1)
  1169. PCR problem - can not get the target SSR band (reply: 1)
  1170. RT-PCR amplification problem - amplification problem (reply: 1)
  1171. PCR problems - (reply: 4)
  1172. Is there anybody use Taq polymerase of Fermemtas - (reply: 20)
  1173. Amplification Problem - PCR Failure... (reply: 5)
  1174. LacZ PCR - (reply: 1)
  1175. Fusion PCR Protocol? - (reply: 6)
  1176. Primer dimers - (reply: 1)
  1177. RT-PCR 1 step - (reply: 2)
  1178. PCR and Primers - PCR-no products (reply: 4)
  1179. AMPLIFYING LONG FRAGMENTS - problems obtaining 3kb fragment (reply: 4)
  1180. Amplification Failure of Unknown Reason - (reply: 1)
  1181. Ammonium Sulfate PCR buffer - Ammonium Sulfate PCR buffer (reply: 1)
  1182. Detection in SmartCycler RT-PCR - (reply: 1)
  1183. Probes for Real-Time PCR - (reply: 3)
  1184. DNA Contamination in RNA? - PCR of RNA (reply: 1)
  1185. Help! DNA contamination in RNA and RT-PCR. - (reply: 1)
  1186. Problems in my Multiplex PCR - (reply: 6)
  1187. PCR detection of water-bornebacteria - (reply: 1)
  1188. Sequencing PCR products - (reply: 2)
  1189. cDNA PCR - Amplifying region using PCR from cDNA library (reply: 1)
  1190. tomato DNA for PCR - (reply: 1)
  1191. Re: dNTP make up - (reply: 1)
  1192. My PCR is not work again - (reply: 6)
  1193. PCR from parraffin embeded samples - (reply: 2)
  1194. Primer design - Primer design for RT-PCR (reply: 1)
  1195. Difficult PCR amplification of 4kb genomic DNA - (reply: 3)
  1196. Primer contamination - (reply: 2)
  1197. PCR on cDNA libraries - (reply: 2)
  1198. Blunting 3' ends with T4 polymerase - (reply: 1)
  1199. Difference between RNase Protention and RT-PCR - (reply: 1)
  1200. A variation of the usual PCRprotocol needed - (reply: 3)
  1201. RNase and PCR - (reply: 1)
  1202. pcr mutagenesis - (reply: 3)
  1203. design a primer - how to determind a specific primer in a complete gonome? (reply: 1)
  1204. dUTP used for PCR - (reply: 6)
  1205. PCR with cell source - (reply: 1)
  1206. In situ PCR and hybridization in plant - In situ PCR/hybridization protocols (reply: 1)
  1207. Difference in RT PCR products when using total RNA or mRNA - (reply: 4)
  1208. PCR mutagenesis - (reply: 1)
  1209. M13 phage amplification,who canhelp? - (reply: 2)
  1210. Poor PCR result - (reply: 2)
  1211. Rt-PCR help,come in - use RT (reply: 2)
  1212. PCR or Southern? - (reply: 3)
  1213. hot start Taq - Taq polymerase (reply: 1)
  1214. How to find/check the highly conservative sequence - RT-PCR primers (reply: 2)
  1215. RT-PCR - (reply: 2)
  1216. pcr on genomic dna ( tailsmouse), HELP !!!! - (reply: 2)
  1217. Bioinfomatics (ePCR) - (reply: 1)
  1218. About Marker - for PCR (reply: 2)
  1219. Trypsin - PCR amplification (reply: 1)
  1220. PCR problem - non specific bands - pcr problem (reply: 8)
  1221. Poor PCR Results - (reply: 3)
  1222. ABI377 Sequencing of PCR products - (reply: 1)
  1223. How to clean agar contamination for colony PCR - (reply: 2)
  1224. PCR problems - (reply: 1)
  1225. PCR restriction site - (reply: 2)
  1226. Single-cell RT-PCR with only ~10-100 Cells - (reply: 2)
  1227. C-MYC AND CYCLIN D1  / RT-PCR - (reply: 2)
  1228. a question about RT-PCR - (reply: 1)
  1229. primer purification - search method for primer purifying (reply: 3)
  1230. How to make PCR  using the gel solution - (reply: 1)
  1231. Software for MSP (methylation-specific PCR) primer design - (reply: 3)
  1232. primer design - Deinococcus (reply: 1)