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PCR Related Discussions

1. to PCR the PCR product - (reply: 3)
   
2. PCR product appear two close band in my gel! - (reply: 3)
   
3. ITP substitutes... what? - can I add ITP to my degenerated primers to lower the degeneracy? (reply: 2)
   
4. Multiplex pcr trouble - (reply: 4)
   
5. no amplification in target gene - (reply: 1)
   
6. need help...how to compare mouse vs human sequences for primer design - (reply: 2)
   
7. Pfaffl Method... - Is there a range of acceptance for the PCR efficieny in Pfaffl method? (reply: 4)
   
8. RT-PCR for small RNAs - (reply: 1)
   
9. PCR problem (great different of Tm in primers) - (reply: 3)
   
10. DNA PAGE - suitability of PCR buffer (reply: 1)
    
11. Which DNA is good for PCR for amplify a certain sequence? cDNA or genomic DNA ? - (reply: 2)
    
12. pcr amplification - right amplification,right sequence but still in dilemma (reply: 4)
    
13. Amplification efficiency - (reply: 3)
    
14. Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
    
15. PCR sequencing/cloning-seq for SNP identification - (reply: 1)
    
16. Tm of PCR primers: one 68, the other 58. Impossible to succeed? - (reply: 3)
    
17. many bands on PCR products - is it unusual? (reply: 6)
    
18. PCR product dimer - clean it up (reply: 3)
    
19. single stranded binding protein? - how to put into my PCR reaction? (reply: 6)
    
20. Primer design for PCR following ChIP - (reply: 3)
    
21. Will salmon sperm DNA in ChIP protocol interfere the WGA amplification? - (reply: 1)
    
22. PCR Inhibitor - Where/What are you? (reply: 9)
    
23. How to name primers? - Any official nomenclature systems to name primers? (reply: 4)
    
24. Primer dimers!? HELP - (reply: 8)
    
25. PCR troubleshooting - (reply: 22)
    
26. MSP primer does not work - (reply: 5)
    
27. PCR PRINCIPLES SOS! - PCR (reply: 9)
    
28. How to increase the product amount in BSP? - weak amplification in BSP (reply: 3)
    
29. How primers can be measured? - (reply: 6)
    
30. general PCR question - (reply: 15)
    
31. RE sites on the primer. - How can I check for restriction sites on a primer sequence? (reply: 2)
    
32. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
    
33. DNA Extraction using Phenol and Ethanol - ETHANOL AND PCR (reply: 7)
    
34. PCR product stuck in agaros wells - (reply: 8)
    
35. RNA contamination in primers - (reply: 2)
    
36. how 2 design rev.transPCR primer? - need functioning protocol.... (reply: 1)
    
37. polyadenylation and RT-PCR - (reply: 2)
    
38. methylnick.please help me with MSP primer designing! - (reply: 3)
    
39. concentration of primers - (reply: 1)
    
40. PCR efficiencies for analysis of relative real time data - (reply: 1)
    
41. Can I still use PCR to select transfectatns - (reply: 1)
    
42. End of PCR products destroyed - (reply: 2)
    
43. RNA PCR - RNA PCR (reply: 4)
    
44. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
    
45. About DAPK1 MSP primer design. - About MSP primer design. (reply: 4)
    
46. Inverse PCR ligation - (reply: 1)
    
47. PCR disappeared - there is no amplification (reply: 11)
    
48. Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
    
49. Oligoarray vs PCR Microarray - (reply: 2)
    
50. deisgning primers with restriction site - (reply: 7)
    
51. Questions about primer design for BSP in plant - shouldn't we take the mC in CHG CHH context into consideration? (reply: 3)
    
52. RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
    
53. RT-PCR works but not consistent - (reply: 3)
    
54. cDNA primer help - (reply: 1)
    
55. cDNA and primer Design Help - (reply: 6)
    
56. Background amplification - (reply: 1)
    
57. methylation specific PCR - (reply: 5)
    
58. Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
    
59. Making blunt ends DNA with T4 Polymerase - (reply: 6)
    
60. Primer design program for Methylight - (reply: 3)
    
61. PCR of ssDNA - (reply: 2)
    
62. repeting PCR failing the reaction - (reply: 2)
    
63. primer design software - primes for full lenth gene (reply: 3)
    
64. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
    
65. pcr amplification 5 kb - (reply: 4)
    
66. PCR efficiency? - (reply: 3)
    
67. Please help me design MSP primer! - (reply: 4)
    
68. PCR fragment ligation - ligation of 3 pcr frangments (reply: 1)
    
69. contmination PCR? - bands in - control but no bands in samples (reply: 2)
    
70. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
    
71. How much oligo dT cDNA to use in PCR - (reply: 3)
    
72. Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
    
73. PCR problem - PCR did not work (reply: 5)
    
74. PCR false positive reasons - (reply: 3)
    
75. Is Klenow necessary for RT-PCR? - (reply: 3)
    
76. PCR cycle - (reply: 1)
    
77. ABI real time PCR instrument fail rate - Wondering whether we need the (expensive) service contract (reply: 5)
    
78. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
    
79. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
    
80. Why am I only getting smears for my genomic DNA long-distance PCR products? - It worked great with sheep BACs but failed miserably for genomic DNA (reply: 4)
    
81. PCR amplification - how to design the PCR program (reply: 4)
    
82. PCR amplification - (reply: 2)
    
83. PCR = products come randomly? - (reply: 4)
    
84. primer sequence - reverse primer sequence (reply: 3)
    
85. PCR cloning problem. Pls help...very urgent! - PCR cloning problem. Pls help...very urgent!!!!!!! (reply: 2)
    
86. RT-PCR GAPDH Variability - (reply: 1)
    
87. cDNA and additional bands when run on gel - I get a band o fthe same Mw regardless of the primers I use (reply: 2)
    
88. PCR Optimization Question - Non-specific smearing around target level? (reply: 6)
    
89. How far advance can you make PCR mix? - I'm a first time poster. (reply: 3)
    
90. PCR troubleshooting - (reply: 10)
    
91. signal amplification and facs - (reply: 1)
    
92. pcr question - (reply: 5)
    
93. Redundancy of real time PCR results - (reply: 2)
    
94. PCR bands - why not straight ?&%#@&& - (reply: 3)
    
95. Help with RT-PCR of 1.4Kb fragment - (reply: 2)
    
96. how to optimize rt-pcr for gene expression analysis - (reply: 3)
    
97. ghost band in PCR? - (reply: 11)
    
98. PCR negative control contaminated and not the other wells - (reply: 4)
    
99. What is my control when running a PCR reaction? - (reply: 2)
    
100. RNA extraction for real time PCR from specific brain region - (reply: 1)
     
101. How to synthesize your own oligo-dT primer - (reply: 4)
     
102. PCR Problem - 300bp skip in the middle (reply: 9)
     
103. A little more help with PCR! - (reply: 2)
     
104. Designing Primers - (reply: 1)
     
105. Basic question about setting up a PCR reaction - (reply: 4)
     
106. Immunostaining - Amplification of signal - (reply: 1)
     
107. basic basic question regarding pcr product storage - (reply: 2)
     
108. rolling circle amplification - (reply: 1)
     
109. mrna detection with sequence specific primers - (reply: 2)
     
110. Normal PCR for looking expression - (reply: 1)
     
111. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
     
112. internal control primers for plants - (reply: 14)
     
113. qRT-PCR primer design - (reply: 1)
     
114. Where do you get your PCR primers from? - (reply: 3)
     
115. Real Time PCR Efficiency - (reply: 3)
     
116. genomic DNA contamination in RT-PCR - (reply: 7)
     
117. PCR primers + large mRNA amplification - (reply: 5)
     
118. how much cDNA do you use for RT-PCR? - (reply: 2)
     
119. gDNA extraction from mouse ear for PCR - "Hot Shot" extraction (reply: 1)
     
120. PCR WOES...... - STRATEGY DOUBT... (reply: 3)
     
121. No PCR product visualized using quikchange kit - (reply: 10)
     
122. Strange amplification pattern in DNA real time PCR - (reply: 4)
     
123. PCR Primers for introducing tag - (reply: 5)
     
124. Could have more DNA concentration inhibition effect on PCR? - (reply: 3)
     
125. help design primer - (reply: 6)
     
126. BLAST Help , Designing primers... - (reply: 5)
     
127. help on PCR! - (reply: 2)
     
128. pyrophosphate generation in PCR - (reply: 2)
     
129. Different efficiency in different samples but the same primer - (reply: 1)
     
130. housekeeping gene in klebsiella pneumoniae for RT PCR - (reply: 2)
     
131. Long range PCR - (reply: 1)
     
132. RT PCR of viral RNA segment - (reply: 1)
     
133. Erroneous PCR product due to plasmid DNA contamination - (reply: 2)
     
134. Primer design and degeneracy - Are synthesized sets identical? (reply: 2)
     
135. Ghost contamination in Real Time PCR - (reply: 4)
     
136. panomics promoter methylation PCR kit - results not what i expected (reply: 1)
     
137. high fidelity PCR polymerase - ask for help! (reply: 3)
     
138. primers amplify additional band (!) - (reply: 1)
     
139. PCR Genotyping Problem - (reply: 7)
     
140. resuspended primers in water - is this a problem?! (reply: 5)
     
141. the mystery of the haunted pcr - Did my new preps degrade? Did my PCR fail? Or something more sinister? (reply: 5)
     
142. Vent polymerase vs Klenow - (reply: 1)
     
143. Do I need sterile RT-PCR tubes? - Real time PCR (reply: 5)
     
144. Designing the primers for qRT PCR - Query - (reply: 14)
     
145. doubts about melting curve, Ct... - understanding results from 1st real time PCR (reply: 5)
     
146. primers sequence - (reply: 2)
     
147. problem with WGA amplification of my chip sample - (reply: 4)
     
148. Chip Troubles - Help: Strong PCR signal from negative control region (reply: 7)
     
149. Image J for PCR analysis - File type (reply: 5)
     
150. primer designing - (reply: 1)
     
151. How to design PCR primer to amplify a very long sequence(about 9.7kb) - I want to amplify cDNA genome of HCV. It's about 9.7kb long (reply: 2)
     
152. wierd rt-pcr - (reply: 1)
     
153. how to check concentration of dNTP I bought - (reply: 3)
     
154. genomic DNA PCR - is it the same as a cDNA PCR (reply: 9)
     
155. Primer Design for nucleotide sequence - (reply: 1)
     
156. spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
     
157. Primer dimer problem in MSP - (reply: 2)
     
158. Unspecific PCR bands - Why that happens? (reply: 5)
     
159. Attaching DNA bases - Creating primers and plasmids? (reply: 3)
     
160. Primer doubts - (reply: 2)
     
161. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
     
162. PCR cloning - (reply: 5)
     
163. PCR mixture - (reply: 2)
     
164. Fast vs Standard PCR - (reply: 1)
     
165. Why the addition of restriction sites to primers? - (reply: 3)
     
166. RT-PCR to amplify ORFs - (reply: 3)
     
167. Primer desing for transcript variant - (reply: 2)
     
168. Transcript variation - measuring by RT-PCR - (reply: 3)
     
169. Primer analysis sites - Any suggestions? (reply: 3)
     
170. Problem with promoter PCR and cloning - (reply: 3)
     
171. RAPD and viruses - RAPD using very very short primers (reply: 4)
     
172. PCR components/DNA sample optimal amounts - ul of DNA and each component for a good reaction. (reply: 10)
     
173. Long Range PCR - (reply: 8)
     
174. please help me to be sure from design primer have it - (reply: 3)
     
175. reverse-transcriptase PCR (internal control?) - (reply: 6)
     
176. heparin in DNA - no working PCR (reply: 4)
     
177. QIAquick PCR purification kit for in vitro transcription? - (reply: 6)
     
178. when to set up a RT-PCR for mRNA expression? - detect transcription of transgene (reply: 1)
     
179. LR PCR and running gels - Roche Expand Long Range dNTPack (reply: 5)
     
180. When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
     
181. checking mRNA expression by reverse transcriptase PCR - (reply: 2)
     
182. Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
     
183. PCR for psoudomonas - (reply: 7)
     
184. resuspend Primers in TE or in water? - (reply: 7)
     
185. how to extract DNA from adenovirus for template of PCR - (reply: 2)
     
186. ssr cross species amplification - (reply: 2)
     
187. Colony PCR with Phusion - (reply: 7)
     
188. Can we measure gene expression with standard PCR? - (reply: 1)
     
189. manual primer desgining - (reply: 2)
     
190. Abgene PCR master mix?-MgCl concentration - (reply: 1)
     
191. DMSO in PCR - (reply: 4)
     
192. Sybr Green real time pcr limit of detection - (reply: 1)
     
193. single band -> multiple banding in PCR - (reply: 2)
     
194. Taq polymerase expressing E. coli requested - (reply: 2)
     
195. real-time PCR problem - (reply: 1)
     
196. BLAST search of primer sequence - (reply: 3)
     
197. newbie question on Primer design for MSP - (reply: 2)
     
198. Best house keeping gene for RT-PCR - (reply: 6)
     
199. Real-time PCR for mRNA stability - (reply: 2)
     
200. RT-PCR inconsistant results - (reply: 1)
     
201. How long can keep PCR product in room temperature? - somebody switched off PCR Machine (reply: 6)
     
202. Oligo dT primer - oligo dT primer with non-T priming 3´ or with 5´ (reply: 3)
     
203. gDNA template for PCR - (reply: 2)
     
204. PRC reagents and equipments - Is a PCR machine robust? (reply: 2)
     
205. annealing times in PCR - (reply: 6)
     
206. Large smear in PCR - (reply: 2)
     
207. Confirming published methylation primers - (reply: 1)
     
208. developing real time PCR - (reply: 5)
     
209. Help designing primers; also help with viewing GenBank - (reply: 3)
     
210. false positives in PCR due to environmental contamination? - (reply: 7)
     
211. I'm beginning PCR..... - (reply: 4)
     
212. Primer design - How to - am a novice ? (reply: 6)
     
213. PCR product size - (reply: 1)
     
214. Primer optimization (with probes) - Urges... (reply: 2)
     
215. Direct PCR from Gram positive bacteria - Is it possible? (reply: 3)
     
216. arms PCR problem - (reply: 2)
     
217. SNP - How to design the SNP primer (reply: 1)
     
218. storing pcr samples - (reply: 19)
     
219. Dilute your cDNA ? - for real time PCR (reply: 2)
     
220. RT-PCR reagents KIT 1 or 2-step? - (reply: 3)
     
221. amplifying small amounts of converted DNA or unconverted DNA? - (reply: 2)
     
222. Extraction of PCR product from gel - (reply: 4)
     
223. How to get GOOD rt-pcr bands - (reply: 7)
     
224. Choice of one-step or two-steps RT-PCR - (reply: 3)
     
225. PCR Puzzle- Unusal Bands - (reply: 13)
     
226. Problems with Bisulfite PCR - (reply: 13)
     
227. when does pcr products degrade like this? - images attached (reply: 7)
     
228. Choosing Primer: random or Olig-dt ? - (reply: 2)
     
229. problem in PCR pdt size - (reply: 4)
     
230. how to synthesize the pcr primers - (reply: 3)
     
231. designing degenerate primer - (reply: 2)
     
232. Washing the spin columns from PCR purekit - (reply: 1)
     
233. incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
     
234. Trouble inserting PCR derived insert into vector - Trouble inserting PCR derived insert into vector (reply: 2)
     
235. how to design pcr primers - (reply: 7)
     
236. RT-PCR primer design and in silico PCR - (reply: 4)
     
237. PCR yield, can assess by absorbance without electroforesis? - (reply: 2)
     
238. Checking mRNA expression by RT-PCR - (reply: 2)
     
239. Primer extension and sequencing from total RNA - (reply: 1)
     
240. primer design - over expression (reply: 1)
     
241. RT-PCR no amplification - (reply: 2)
     
242. Bisulfite PCR Question - (reply: 2)
     
243. PCR bias with BSP primers - (reply: 1)
     
244. quadraplex RT-PCR error - (reply: 1)
     
245. final extension real time PCR - (reply: 4)
     
246. cloning of a fluorecent PCR product - (reply: 1)
     
247. pcr shelf life? - (reply: 1)
     
248. NOT SyBrGreenPCR amplification in positive control-Why? - (reply: 1)
     
249. Strange amplification curve - (reply: 2)
     
250. how to improve PCR yield? - (reply: 5)
     
251. PCR a fragment of 6kb - (reply: 14)
     
252. TEST: embed video - PCR song - (reply: 4)
     
253. Primer design and tolerances - (reply: 3)
     
254. PCR to sequencing problems - (reply: 5)
     
255. PCR Performance Test - QC test for new Taq Polymerase (reply: 3)
     
256. EXOSAP-IT degrades my PCR products - (reply: 3)
     
257. Internal Standards for RT-pCR - (reply: 11)
     
258. Problems with amplification - (reply: 7)
     
259. Can a 96 well plate for rt PCR be used for ELISA? - 96 well plate , ELISA (reply: 2)
     
260. PCR master mix preparation - (reply: 11)
     
261. primer design - (reply: 2)
     
262. real time pcr newbie - (reply: 3)
     
263. help with degenerate primer design - (reply: 1)
     
264. PCR: Big Primers = Big Problems (for me) - I'm really not sure what's going on in those poor little tubes... (reply: 2)
     
265. Questions about PCR amplification using cDNA (RT from total RNA). - (reply: 6)
     
266. mutations in primer sequence - (reply: 2)
     
267. How to get perfect amplification peaks - Share my long QPCR troubleshooting experience (reply: 1)
     
268. Amount of DNA to run on gel - Be it from PCR or from DNA extraction steps (reply: 4)
     
269. digestion of pcr - (reply: 2)
     
270. Amplification Effeciency - solve the problem with E value (reply: 2)
     
271. Recombinant PCR - (reply: 1)
     
272. PCR Inhibition by DNA? - (reply: 5)
     
273. pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
     
274. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
     
275. Nested primers - (reply: 5)
     
276. Total RNA isolation for RT-PCR - (reply: 3)
     
277. Primer design - Primer design for PCR with TE sites (reply: 2)
     
278. Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
     
279. PCR and competent cells - (reply: 3)
     
280. primer conditions for expression work - (reply: 5)
     
281. DNA polymerase fidelity assay (NOT PCR-based) - (reply: 2)
     
282. Failed RT-PCR amplification of 3-&5''-ligated small RNAs - (reply: 5)
     
283. RT-PCR for various sample with Tm from 45-60 degree - (reply: 1)
     
284. Question about primer design targeting GC-rich region - (reply: 2)
     
285. promoter pcr using primers with restriction sites giving trouble - (reply: 7)
     
286. how to optimize primer and cDNA concentration for real-time - (reply: 2)
     
287. primer Question pVax1 RE / Kozak - (reply: 1)
     
288. Help -designing primers for real time RT-PCR - (reply: 1)
     
289. Is it possible to use a PCR cleaning kit to clean a band from a TAE-Agarose gel? - (reply: 1)
     
290. getting long pcr from cDNA - (reply: 4)
     
291. How many bases should be added to end of enzyme in PCR for effective cutting - (reply: 8)
     
292. PCR from cDNA library - (reply: 1)
     
293. help for long PCR product amplification - (reply: 4)
     
294. clear culture, but PCR positive - (reply: 4)
     
295. Primer design for SNP detection - (reply: 2)
     
296. Bad primer efficiency during multiplex qPCR - (reply: 1)
     
297. problems with low concentration DNA templates for PCR - (reply: 5)
     
298. phophorylating PCR product after CIP - (reply: 2)
     
299. re-pcr (2nd) different with pcr (1st) - (reply: 5)
     
300. pcr template amount - (reply: 2)
     
301. Inefficient RT-PCR, ¿bad cDNA? - don't know why (reply: 1)
     
302. PCR with paraffin-embedded tissue DNA samples - I have had problems to amplify DNA samples from paraffin-embedded tiss (reply: 1)
     
303. max primer length - 85 nt too long? - (reply: 5)
     
304. Problems with amplification curve - (reply: 5)
     
305. how to isolate ORF by PCR - (reply: 1)
     
306. different transcription from 5' UTR than 3' UTR primers - (reply: 8)
     
307. how to isolate gene through PCR - (reply: 3)
     
308. recommended primers for caspase 8 & 9 - human (reply: 4)
     
309. UBC primer PCR - (reply: 1)
     
310. Optimising bisulphite PCR primers - (reply: 4)
     
311. It is nessesary to apply aseptic technique for PCR? - (reply: 3)
     
312. Strange large bands on my PCR gel, has anyone seen this before? - PCR (reply: 3)
     
313. Primer design help - secondary structures - Please help me troubleshoot? (reply: 12)
     
314. help with RT-PCR - DNase usage - (reply: 2)
     
315. Help: Genomic DNA PCR - (reply: 3)
     
316. PAGE with Realtime PCR product (Sybr Green) - Post-staining with ethidium bromide? (reply: 4)
     
317. sequential PCR and purification before TA cloning - (reply: 1)
     
318. PCR inhibition in FFPE DNA - (reply: 1)
     
319. pcr template - (reply: 4)
     
320. Determine specific primers and transgene copy number by PCR - (reply: 1)
     
321. optimization of PCR conditions - to know that primers are specific and detect copy number (reply: 1)
     
322. PCR with Betaine, DMSO, BSA, DTT - (reply: 5)
     
323. Any Universal Primer for 3' of cDNAs of all mRNAs? - (reply: 2)
     
324. A couple of (RT-PCR) cowboy questions - I feel the need, the need for speed (reply: 2)
     
325. generating an array by gene amplification - (reply: 2)
     
326. PCR on this gene - products always present? - (reply: 9)
     
327. better primer for cDNA synthesis in real time pcr - (reply: 1)
     
328. PCR cycle for 80 bases - (reply: 2)
     
329. genome amplification before cloning? - (reply: 2)
     
330. High concentration of antibiotics in amplification of plasmids - concerns about mutation of inserts in plasmid (reply: 1)
     
331. can you judge a 22bp primer based on the 3' 10bp? - (reply: 6)
     
332. my negative PCR control is still positive ! - (reply: 32)
     
333. Is nested PCR always neccisary? - (reply: 3)
     
334. Getting primer dimers in RT-PCR on and off? - (reply: 2)
     
335. Internal control for RT-PCR analysis - The expression of internal control (reply: 3)
     
336. PCR reaction - quick question! - (reply: 2)
     
337. Quantitative PCR question - Comparative quant PCR with 16S rRna as reference gene (reply: 2)
     
338. conc of cDNA to be used - PCR to test primers (reply: 5)
     
339. What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR (reply: 3)
     
340. optimum conditions for long pcr - (reply: 1)
     
341. free programme for RT-PCR quantification - free programme for RT-PCR quantification (reply: 3)
     
342. DIG pcr labeling and northern blot - (reply: 2)
     
343. Primer for all HPV - (reply: 4)
     
344. Weekly Decontamination Routine? - To prevent DNA contamination in a PCR lab (reply: 1)
     
345. please help me check BSP primers. - (reply: 6)
     
346. PCR on template with trinucleotide repeats - PCR tips for complexe DNA template (reply: 4)
     
347. DNA PCR template - GC content (reply: 1)
     
348. 1:10 serial dilution in rt-pcr with slope of -0.58 ... - (reply: 1)
     
349. using piece agarose band directly on PCR - does it work? - (reply: 3)
     
350. Lengthy primers - (reply: 11)
     
351. primer design - how far apart Tms could be? (reply: 4)
     
352. positive colony PCR, but no insert!? - (reply: 5)
     
353. Taq polymerase - (reply: 1)
     
354. Steps in PCR - (reply: 1)
     
355. PCR detection for latent infection of Human CMV - (reply: 1)
     
356. ORF region amplification problem - (reply: 2)
     
357. Free sample of GoTaq® Hot Start or any GoTaq® DNA Polymerase - (reply: 1)
     
358. need help on microRNA real-time PCR - (reply: 6)
     
359. Help for PCR - (reply: 1)
     
360. PCR Help! - Primers-quality checking software (reply: 3)
     
361. standard PCR efficiency - No real time! (reply: 2)
     
362. design primers - (reply: 3)
     
363. a problem about the Tm of the BSP primer - (reply: 2)
     
364. DNA polymerase that produces blunt end and doesnt smear! - (reply: 2)
     
365. Help! ignoring bad results in standard curve - about the pcr efficiency... (reply: 1)
     
366. PCR on Genomic DNA from cell lysate - (reply: 5)
     
367. PCR improvement - how to get more PCR product! (reply: 6)
     
368. Primers distinguishing splicing alternatives - (reply: 1)
     
369. Amplifying large gene - (reply: 2)
     
370. Problem with bands of PCR products - (reply: 2)
     
371. no more PCR amplification after I got rid of a contamination - (reply: 4)
     
372. about PCR efficiency - (reply: 2)
     
373. RT-PCR or disulfite treatment, which is better? - (reply: 5)
     
374. TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
     
375. Sequencing result is vector self-ligation while bacterial PCR identification was - (reply: 5)
     
376. primer design from mRNA - (reply: 12)
     
377. who can give me a hand to check the BSP primer? - (reply: 5)
     
378. How do you prevent PCR evaporation? - (reply: 9)
     
379. Please help me design BSP primer. - (reply: 14)
     
380. PCR contamination - (reply: 1)
     
381. bisulfite PCR: is it my product? - (reply: 5)
     
382. How can I create a T vector for PCR product cloning - (reply: 2)
     
383. Should Taq polymerase be resuspended before use? - (reply: 3)
     
384. PCR product won't come down from well - (reply: 3)
     
385. Primer design: how do I add a restriction site to my primer? - (reply: 2)
     
386. RE of PCR Fragment - (reply: 2)
     
387. Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
     
388. Starting with semi quantitative RT-PCR and a little bit lost.. - (reply: 4)
     
389. Help: PCR clone problem - (reply: 2)
     
390. Storing primers...together? - (reply: 3)
     
391. Genomic DNA fragment labeling by random priming - (reply: 1)
     
392. different length for primers amplifying - (reply: 2)
     
393. primer dilution - primer dilution from picomoles to micromolar (reply: 2)
     
394. cytosolic PCR control - (reply: 1)
     
395. PCR doesn´t work anymore - (reply: 3)
     
396. Primer concentration is quantitative Real-time PCR - (reply: 1)
     
397. Real time pcr in Rotor gene 6000- not getting positive results - (reply: 1)
     
398. primer dimer annealing temperature - (reply: 2)
     
399. design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
     
400. What is the function of acetate solution in DNA extraction for PCR used - (reply: 6)
     
401. TetR primer - (reply: 1)
     
402. choosing real-time PCR machine - (reply: 13)
     
403. Sproadic PCR failures - (reply: 5)
     
404. Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
     
405. verify my microarray data - need help in real time PCR (reply: 1)
     
406. some primer dilution calculations - (reply: 7)
     
407. Band Intensity - Linear or Exponential? PCR Products - (reply: 4)
     
408. PCR - reduced binding efficiency with lower Tm? - (reply: 2)
     
409. Improving PCR specificity? - (reply: 2)
     
410. Purified PCR product - (reply: 2)
     
411. PCR product too short - (reply: 6)
     
412. Housekeeping genes for RT-PCR in soybean - (reply: 6)
     
413. primer tm with mismatch? - (reply: 6)
     
414. cDNA quantification for PCR - (reply: 5)
     
415. TOPO primer choice for sequencing - (reply: 3)
     
416. no PCR product - (reply: 14)
     
417. Real-Time PCR and Housekeeping gene - (reply: 9)
     
418. Can do reverse transcription by gene-specific primers? - (reply: 3)
     
419. Controls for real-time PCR... again - (reply: 7)
     
420. Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
     
421. primer design with RE sites - (reply: 4)
     
422. plz save my PCR product ! - (reply: 4)
     
423. Transcript Amplification in genomic DNA PCR - (reply: 1)
     
424. primer desighning with RE sites - (reply: 5)
     
425. Taqman PCR master mix - (reply: 4)
     
426. Primer design (long transcripts) - (reply: 2)
     
427. PCR faint bands under UV light - (reply: 10)
     
428. Primers for Real time PCR - Really need some advice (reply: 1)
     
429. PCR Help - 2kbp product - (reply: 12)
     
430. RNA amplification - (reply: 3)
     
431. whole genomic amplification - (reply: 6)
     
432. PCR product of unlinearized plasmid - (reply: 1)
     
433. Stem-loop RT primers: how to anneal? - (reply: 1)
     
434. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
     
435. second strand cDNA synth without primer? - (reply: 2)
     
436. do you put two pairs primers in one tube when you do site mutations? - (reply: 2)
     
437. General question about restriction sequences in PCR primers - (reply: 3)
     
438. Will TOPO TA cloning work on "old" purified PCR product? - (reply: 4)
     
439. SYbrgreen PCR protocol for the rookie - (reply: 1)
     
440. Can PCR on same sample give different results when repeated? - (reply: 2)
     
441. GAPDH primer sequence - cloning of huGAPDH gene (reply: 2)
     
442. RT PCR for methylation analysis - (reply: 3)
     
443. After detect PCR cannot get any colony with right direction of insert - insert direction (reply: 2)
     
444. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
     
445. Induction by IPTG of T7-RNA polymerase in BL21 strains : time lag? - (reply: 2)
     
446. chip assay primers - chip assay primers (reply: 2)
     
447. Genotyping PCR - (reply: 3)
     
448. suitable length DNA for PCR? - (reply: 2)
     
449. Setting up and analysing real time PCR - (reply: 2)
     
450. how to add bases to the primer restriction sites - (reply: 2)
     
451. PCR inespecific band amplification - (reply: 2)
     
452. Problems with making mix of PCR reagents ? - (reply: 4)
     
453. Questions about Stratagene QickChange Multi-mutagenesis - about how many primers and template DNA size (reply: 5)
     
454. primer design - (reply: 3)
     
455. PCR - Anomalous mobility of PCR product (reply: 2)
     
456. SYBR green PCR - (reply: 4)
     
457. Melting curve - SYBR Green PCR (reply: 8)
     
458. PCR problem, no amplicon, tried everything I could think of.. - (reply: 6)
     
459. Anyone know how can I precipitate PCR product in 96-well plate? - (reply: 2)
     
460. How to ressuspend primers for Sybr? - (reply: 3)
     
461. Ligation problem with PCR product cloning - (reply: 9)
     
462. simple primer calculation - (reply: 5)
     
463. WGA problems CpG island amplification - (reply: 6)
     
464. What's wrong with my pcr? - high temperture with low specificity (reply: 6)
     
465. PCR cloning-primer design and ligation - (reply: 20)
     
466. RT-PCR Vs qRT-PCR help please! - (reply: 4)
     
467. picking primers - (reply: 4)
     
468. 300 bp more after PCR purification - please help - (reply: 3)
     
469. 16s primers and PCR - (reply: 1)
     
470. primer/probe mix for standard generation? - (reply: 2)
     
471. Primers from RT-PCR database - RT-PCRdatabase primers giving primer dimers (reply: 1)
     
472. Fast way to get plasmid for PCR - (reply: 2)
     
473. restriction enzyme site with primers - (reply: 4)
     
474. PCR efficiency for qRT-PCR analysis - PCR efficiency for qRT-PCR analysis (reply: 4)
     
475. PCR product cloning - (reply: 6)
     
476. PCR failure. GC problem? - (reply: 7)
     
477. minimum mRNA concentration for RT-PCR - (reply: 4)
     
478. Help. Trying to sequence my plasmids, but the primers refuse to prime! - (reply: 1)
     
479. weird problem with Primers! - problem with the gene sequence i used to design my primers (reply: 4)
     
480. Degnerate PCR and Sequencing Reactions - (reply: 4)
     
481. pcr issue-gene not amping - (reply: 3)
     
482. Forward n reversre primer Tm difference is 10 degree - (reply: 1)
     
483. Primer mismatch bother me! - when enzyme sites added at 5' ends, mismatch increases. (reply: 1)
     
484. Smearing in PCR - (reply: 4)
     
485. Troubleshooting RT-PCR - (reply: 7)
     
486. Why using heated-top or add mineral oil in PCR - (reply: 2)
     
487. do I get right PCR product - (reply: 20)
     
488. primer design (downloadable) programs - not online tools (reply: 4)
     
489. yeast colony PCR - How to improve yeast colony PCR (reply: 7)
     
490. BLOOD storage and PCR problems! HELP - (reply: 1)
     
491. pcr problems - pcr to solve (reply: 2)
     
492. PCR water contamination - (reply: 5)
     
493. Thermostable Reverse Transcriptase for RT-PCR - (reply: 3)
     
494. figure out the size of amplicon using known primer - (reply: 3)
     
495. real time PCR data analysis - method I have never heard of before (reply: 3)
     
496. Primer concentration... - (reply: 5)
     
497. microgram per microlitre primer to molarity conversion.. - calculation plss (reply: 1)
     
498. How to amplify PCR product - wonder why using linear fragment as template, PCR result was always sm (reply: 1)
     
499. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
     
500. Long PCR primers for epitope tagging - where to order? (reply: 3)
     
501. PCR screening for recombinant clones - (reply: 1)
     
502. Extremely Low Yield PCR with restriction site addition - Assistance requested for beginning molecular biologist on PCR techniqu (reply: 3)
     
503. ***MSP Primer Troubleshooting Specificity*** - (reply: 6)
     
504. Problems amplifying unstable construct in E.coli - Tips needed (reply: 2)
     
505. false positive in colony screening PCR? - (reply: 8)
     
506. RT-PCR consistency - (reply: 2)
     
507. No PCR product with verified primers on templates - Where is the bug???? (reply: 2)
     
508. dried out pcr product - (reply: 1)
     
509. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
     
510. Please see the PCR electropherogram - What's wrong with my pcr? (reply: 8)
     
511. How good are my PCR primers? can I go with it?! - (reply: 2)
     
512. PCR inhibitors - help ??!! (reply: 8)
     
513. Bands in Negative control PCR - (reply: 18)
     
514. QPCR primer Pairs - (reply: 1)
     
515. primer design for unknown cDNA sequence - (reply: 1)
     
516. Random Amplification + TA cloning = HATE - (reply: 2)
     
517. touchdown qPCR - using degenerative primers (reply: 2)
     
518. Quikchange PCR dNTP mix? - (reply: 8)
     
519. primer Tm for PCR mutagenesis - (reply: 1)
     
520. Inconsistent bisulfite PCR - (reply: 8)
     
521. Restricition enzyme qouted in paper doesnot cut the PCR product according to the - (reply: 3)
     
522. ISH vs. Real Time PCR - correlation between CT-values and quantity - (reply: 2)
     
523. 96 WELL PCR Purification Suggestions - (reply: 4)
     
524. no PCR product! any advice? - (reply: 14)
     
525. Identification of monomers/dimers in whole cell lysate via SDS-PAGE - (reply: 3)
     
526. New to rt pcr real time - guidelines and advice (reply: 3)
     
527. what to do when Tm > annealing temperature for PCR - (reply: 8)
     
528. overlap extension pcr - (reply: 1)
     
529. Polymerase for Quikchange mutagenesis - (reply: 3)
     
530. Quikchange mutagenesis primer and PCR design - (reply: 3)
     
531. Primer design - Issues with GAPDH primer blast search (reply: 2)
     
532. PCR inhibitors in soil DNA - (reply: 6)
     
533. primers dont match FASTA gene sequence... 'm confused - (reply: 11)
     
534. How to know tissue specific gene expression by real time PCR - (reply: 3)
     
535. sequence retrieval for primer design - (reply: 2)
     
536. Statistics: real-time RT-PCR - (reply: 4)
     
537. RT-PCR using formaldehyde cross linked samples - RNA extraction using CHIP crosslinked samples (reply: 1)
     
538. PCR of AT-rich DNA - (reply: 3)
     
539. phantom non specific amplification at high ct values - (reply: 7)
     
540. Primer conc - (reply: 6)
     
541. cDNA cloning using Taq/pfu polymerase - (reply: 2)
     
542. Non-specific Real time PCR signals - (reply: 1)
     
543. Mutagenesis PCR transforming into BL21(DE3) - (reply: 2)
     
544. Does anyone know how to design LUX primers - (reply: 2)
     
545. PCR amplificaiton isnt working - (reply: 12)
     
546. Problems on overlap extenstion PCR - (reply: 2)
     
547. A-overhang in PCR product - (reply: 2)
     
548. Cox-2 primer for PCR - method how to search good cDNA in Pubmed (reply: 1)
     
549. How to purify 700bp pcr product? - (reply: 5)
     
550. ChIP PCR problems - I can't get rid of the smears! - (reply: 6)
     
551. why my pcr failed? - (reply: 5)
     
552. PCR primers for CRISPR - primer3 isn't helpful in this case (reply: 1)
     
553. primer design for subclone - (reply: 2)
     
554. QPCR versus regular PCR for Chip - (reply: 2)
     
555. Could you run Taqman PCR on gel to see the band? - (reply: 1)
     
556. Is it necessary to measure the amount of DNA before PCR? - (reply: 3)
     
557. Polymerase - (reply: 3)
     
558. miRNA real time PCR - miRNA real time PCR (reply: 2)
     
559. Mutagenesis and primer purity? - (reply: 4)
     
560. DNA amplification for chip-on-chip - WGA or LM-PCR or others? (reply: 12)
     
561. Trouble-shooting PCR with Origene vectors - I cannot get PCR to work, and I have tried many approaches (reply: 11)
     
562. PCR primer design, real time versus old school - (reply: 2)
     
563. Faint Band - PCR reaction - (reply: 2)
     
564. Assembly PCR problem - (reply: 3)
     
565. PCR TROUPLESHOOTING - (reply: 3)
     
566. Amplifying cultures - quick question - (reply: 3)
     
567. Real TIme PCR problems - (reply: 2)
     
568. I have a problem with the M13 tailed primer method - M13 tailed primers-SSR (plants) (reply: 1)
     
569. Fusion PCR problem: smear! - (reply: 3)
     
570. PCR Tubes - what kind of lid ? (reply: 6)
     
571. problem with unspecific PCR product - (reply: 3)
     
572. What in ChIP samples inhibit PCR? - (reply: 2)
     
573. PCR variability or bad cell source? - (reply: 1)
     
574. Storage of a plate for real time PCR - Ever stored your already prepared plate for real time PCR? (reply: 7)
     
575. RNA polymerase II antibody recommendation? - (reply: 2)
     
576. specific PCR polymerase - (reply: 3)
     
577. RT-Pcr without RNA isolation? - (reply: 3)
     
578. help with site-directed mutagenesis - What polymerase and cells to use? (reply: 3)
     
579. PCR to produce 1.6kbp insert in fragments - (reply: 5)
     
580. MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN - (reply: 1)
     
581. RT PCR Standard curve amplification problem - (reply: 1)
     
582. cloning 708bp PCR prodyct into pET101 vector (TOPO) - (reply: 2)
     
583. Pfu + Taq polymerase and poly-A tails - (reply: 8)
     
584. Primer3 results - (reply: 2)
     
585. Help for Real-time PCR equipment choice - (reply: 4)
     
586. Problems with several unspecific bands after PCR - (reply: 2)
     
587. PCR purification /DMSO / vanishing DNA - (reply: 2)
     
588. Colony PCr - (reply: 4)
     
589. PCR contamination - (reply: 9)
     
590. Cloning PCR product blunt + restriction site - (reply: 2)
     
591. help in cloning 6kb pcr fragment into topo? - (reply: 5)
     
592. cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2)
     
593. PCR of lambda phage - (reply: 1)
     
594. real time pcr with RNA co-precipitated with glycogen - (reply: 3)
     
595. Checking Insert using PCR from plasmid DNA? - (reply: 4)
     
596. transfect from full plasmid and transfect from PCR product - (reply: 5)
     
597. Problems with TA cloning of bisulfite converted PCR product - (reply: 2)
     
598. PCR problem..... - (reply: 4)
     
599. urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
     
600. PCR primers with overhangs - (reply: 2)
     
601. pcr contamination - (reply: 4)
     
602. How to reduce the dimers - (reply: 3)
     
603. Digest-Ligase-PCR Problems (T-DNA fingerprinting gone wrong) - After ligating digested product and doing PCR on it i get no DNA smear (reply: 2)
     
604. magnesium concentration in pcr - (reply: 4)
     
605. Unspecific PCR problem - (reply: 2)
     
606. Primer designing - (reply: 1)
     
607. Designation of PCR primers in ChIP experiment - (reply: 3)
     
608. What is your favorite tool for primer design ? - (reply: 2)
     
609. Routine PCR isn't working - (reply: 3)
     
610. Primer express? - Do you use it? (reply: 2)
     
611. Bad primer design ? How can I know ? - How could I know if a primer would recognize a particular sequence ? (reply: 2)
     
612. The best reverse transcriptase for RT PCR - (reply: 5)
     
613. What is the proper upper limit of false priming in real time? - (reply: 3)
     
614. Ooops, forgot to add Mg to PCR! - But samples amplified! Can I use them? (reply: 3)
     
615. ancient DNa and PCR - (reply: 1)
     
616. Genotyping - Primers for PCR genotyping (reply: 5)
     
617. Real TIme PCR problems - (reply: 3)
     
618. Enzyme sites on Primer - (reply: 3)
     
619. PCR to identify Ig heavy chains (as a diagnostic for immune response) - (reply: 4)
     
620. PCR troubleshooting - (reply: 1)
     
621. PCR of mitochondrial fraction of nuclear-coded mitochondrial proteins - (reply: 4)
     
622. gene-specific Primer for qRT-PCR - (reply: 2)
     
623. BSA in PCR reaction - (reply: 1)
     
624. how to explain this strange amplification chart? - the Ct value comes to be very low because of the unusual amplification (reply: 1)
     
625. Designing RT-RCR Primers according to other species' homology - Using conserved regions as a guide when mRNA isn't known in your a (reply: 1)
     
626. PCR Troubleshooting - (reply: 2)
     
627. Nested PCR - (reply: 5)
     
628. No signal in Real-time but in normal PCR?! - (reply: 2)
     
629. primer design for PCR cloning - (reply: 1)
     
630. What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb? (reply: 6)
     
631. gDNA contamination in realtime PCR - (reply: 4)
     
632. deletion PCR? - I need help (reply: 1)
     
633. your experience with long range PCR - (reply: 3)
     
634. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
     
635. Real-time PCR low efficiency - (reply: 1)
     
636. PCR troubles - PCR works one day but not the next (reply: 10)
     
637. pcr product purification - (reply: 7)
     
638. PCR from cell culture - to detect the presence of adenovirus (reply: 2)
     
639. PCR mix for genotyping - (reply: 1)
     
640. Wrong PCR product after Bisulfite Treatment-reason? - (reply: 1)
     
641. DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
     
642. PCR smear with CHIP - (reply: 2)
     
643. cassette pcr - (reply: 2)
     
644. Smear in PCR after ChIP - (reply: 2)
     
645. I need an animation of a PCR reaction - (reply: 4)
     
646. What happens if different templates dilution in Real-time PCR reaction - (reply: 1)
     
647. colony PCR screening, drove my crazy! need ur suggestion - (reply: 2)
     
648. PCR amplification of region with many ATT repeats - (reply: 1)
     
649. Unable to ligate two PCR products - (reply: 4)
     
650. PCR primers artifact - (reply: 2)
     
651. Purification of digested PCR product (a smear fr 200bp to 3kb) - (reply: 1)
     
652. weak RT-PCR product - (reply: 2)
     
653. qPCR primer efficiancy - (reply: 2)
     
654. How many cycles in PCR would you recommend for ChIP - (reply: 3)
     
655. Primer Express by ABI - (reply: 2)
     
656. real time PCR template - template concentration (reply: 3)
     
657. High Ct Values, low amplification - (reply: 3)
     
658. primer design for site-directed mutagenesis - (reply: 3)
     
659. using nanodrop to measure PCR product - (reply: 3)
     
660. what affects primer specificity? - (reply: 2)
     
661. Assembly PCR - Design of PCR program for primers w/low Tm? (reply: 2)
     
662. modification of PCR buffers - When and how (reply: 1)
     
663. Templiphi amplification kit: How to determine the amount of DNA product - (reply: 2)
     
664. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
     
665. Primers PCR - storage (reply: 5)
     
666. Dealing with VERY Mg sensitive PCR reactions - (reply: 4)
     
667. MSP: M and U primers products - (reply: 1)
     
668. Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
     
669. Multiple bands in PCR - (reply: 6)
     
670. Can I use different amount of cDNA template to run real time PCR? - when the amount of templates are different,Can I use reference gene to (reply: 2)
     
671. PCR from genomic DNA - (reply: 3)
     
672. Human Epigenome Project primers? - (reply: 2)
     
673. Creating short dsDNA from primers for cloning - (reply: 2)
     
674. Degenerate Primers - Help with Degenerate Primers (reply: 1)
     
675. RNA polymerase a sa fractionation control? - (reply: 3)
     
676. PCR screening of colonies - (reply: 3)
     
677. problems with mutant PCR - problems with mutant PCR (reply: 1)
     
678. Colony PCR running as smears - (reply: 1)
     
679. Trouble with doing DNA Isolation and then running a PCR - (reply: 6)
     
680. PCR: Can you get amplification if only one primer anneals? - Same size pcr product using 2 different primer sets (reply: 8)
     
681. 10 fold dilutions show no RT-PCR product whatsoever - (reply: 1)
     
682. RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
     
683. RNA handling for RT-PCR? - (reply: 4)
     
684. PCR cloning: neg self-ligation control, many colonies but no insert - (reply: 3)
     
685. phage cDNA library screening with PCR - (reply: 3)
     
686. PCR help! - urgent (reply: 5)
     
687. PCR trouble: about the annealing temperature - (reply: 4)
     
688. ready to use primers for BSP - (reply: 4)
     
689. PCR PROTOCOL FOR A BEGINNER - (reply: 11)
     
690. Troubles in PCR the converted DNA in promoter region - (reply: 6)
     
691. taqman is giving initially raised then fall off amplification curve why? - changed template concentraion n baseline too.....but ? (reply: 2)
     
692. Are there any special rules for primer design? - (reply: 5)
     
693. what's the difference between sequencing primer and cloning one. - confused (reply: 5)
     
694. ELISA in a real-time PCR plate - possible? (reply: 3)
     
695. sequencing issue - Internal primers (reply: 9)
     
696. GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
     
697. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
     
698. Unnatural smear in PCR product - (reply: 7)
     
699. Can we compare traditional RT-PCR results with Real Time RT-PCR? - (reply: 1)
     
700. ChIP PCR help, maybe is the problem of SDS. - (reply: 4)
     
701. FGF20 PRIMER - (reply: 2)
     
702. Colony PCR - (reply: 8)
     
703. normality distribution in data - real-time PCR (reply: 2)
     
704. how to check normal distribution of data - real-time PCR data (reply: 2)
     
705. Cleaning up PCR products for BSP - Any experience with ExosapIt? (reply: 5)
     
706. Insert PCR - (reply: 1)
     
707. qPCR for microRNAs - minimal primer length? (reply: 6)
     
708. mutant PCR - (reply: 4)
     
709. Ligation of purified PCR product to Topo plasmid (with T7 promoter) and Transfor - (reply: 2)
     
710. DNA purification technique - After a PCR (reply: 8)
     
711. plant genomic DNA isolation and PCR - does CTAB affect PCR ? (reply: 4)
     
712. primer design for Syber Green - (reply: 1)
     
713. primer sets to do pyrosequencing - (reply: 3)
     
714. DGGE - PCR and gel conditions - (reply: 2)
     
715. not reproducible : reverse - transcription PCR - (reply: 5)
     
716. PCR machine choice: - (reply: 14)
     
717. primer walking design tool - (reply: 1)
     
718. PCR OF HIV genome - (reply: 1)
     
719. Agarose gel concentration for small and similar pcr pdts - (reply: 7)
     
720. Cloning a 60 bp pcr product and Mixing Pfx and Taq polymerases - (reply: 4)
     
721. PCR cloning problems - argh!!! (reply: 5)
     
722. optimizing PCR for low Tm primers - (reply: 1)
     
723. primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
     
724. Minimal nucleotide for Taq polymerase binding - (reply: 1)
     
725. PCR efficiencies calculation - (reply: 2)
     
726. primers TM - (reply: 8)
     
727. do you subtract gel base intensity from PCR band intensity? - urgent require (reply: 3)
     
728. pcr product degradation - (reply: 1)
     
729. any problems with RT-PCR for large amplicon sizes? - very strange amplification curves (reply: 2)
     
730. Colony PCR - (reply: 11)
     
731. Degenerate Primers and dI - (reply: 5)
     
732. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
     
733. pcr mutagenesis - (reply: 4)
     
734. designing a primer - (reply: 5)
     
735. preference for region of primer design for qRT-PCR - (reply: 1)
     
736. using primers programms - (reply: 9)
     
737. problem rt pcr long gene - (reply: 4)
     
738. qPCR housekeepers - primers or probes? - (reply: 1)
     
739. gDNA PCR - for promoter amplification (reply: 1)
     
740. pcr for gene fusion - (reply: 3)
     
741. PCR Trouble Shooting - new primer, wrong dilution of primers, wrong band, and many more (reply: 15)
     
742. basic question regarding PCR - (reply: 7)
     
743. Ligation of pBBR with PCR fragments doesn't work - (reply: 3)
     
744. freaky primers - (reply: 5)
     
745. long range PCR primer design - (reply: 1)
     
746. How do you design primers of PCR-STR used in Forensic Medicine - Urgent!!!! (reply: 2)
     
747. Long Range PCR - (reply: 3)
     
748. what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
     
749. Primer tag info - (reply: 1)
     
750. How to get rid of primer dimers - (reply: 2)
     
751. Free sample from Takara. - All kind of polymerase for PCR and real-time PCR. (reply: 7)
     
752. problems with PCR primers - (reply: 15)
     
753. PCR of pBluescript unknown insert - (reply: 1)
     
754. one nucleotide in the primer is wrong - (reply: 2)
     
755. Primer Dilution. ...water or buffer? - (reply: 15)
     
756. How PCR product becomes gelly? - (reply: 1)
     
757. Nested PCR for BSP question - (reply: 4)
     
758. Flag Tagging a PCR product - (reply: 2)
     
759. What is the Ct standard deviation for the same sample in the whole pcr plate? - (reply: 2)
     
760. genomic DNA extraction - for PCR (reply: 1)
     
761. RANDOM PCR PROTOCOL? - Looking but no finding... (reply: 1)
     
762. PCR thermocycler (Eppendorf) - noise problem (reply: 1)
     
763. has any guys here had experience with poly(A) polymerase - (reply: 1)
     
764. real time pcr in a day - (reply: 5)
     
765. Help me for DNA concentration for PCR - (reply: 4)
     
766. PCR efficiency - (reply: 2)
     
767. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
     
768. Triton X in PCR - (reply: 7)
     
769. PCR unspecific amplification - (reply: 10)
     
770. real time PCR using two sets of primers with different melt curves - (reply: 4)
     
771. Overcoming PCR bias before bisulfite sequencing?! - (reply: 2)
     
772. AmpliTaq Gold - high fidelity polymerase? - (reply: 2)
     
773. What type of PCR to identify a plasmid insert? - (reply: 4)
     
774. Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
     
775. problem in getting results from a optimized PCR - (reply: 5)
     
776. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
     
777. Howto: Maximum possible yield from a PCR? - What to optimize: Mgcl/primers/Polymerase/enhancers? (reply: 4)
     
778. Introduce restriction sites with PCR primers - (reply: 2)
     
779. Real-time PCR optimization - (reply: 8)
     
780. How much DNA in PCR? - (reply: 6)
     
781. Different sequencing result of PCR products from the same cDNA - (reply: 2)
     
782. Free FastStart Universal Real-Time PCR Master Mixes from Roche - (reply: 7)
     
783. PCR problems when amplifying sequence from gDNA - amplification of ~1.2 kb from gDNA (reply: 7)
     
784. primer flaw I'm missing? - (reply: 7)
     
785. MSP Primer design -rules to choose primers and reasons - (reply: 2)
     
786. Blunt end cloning PCR product - (reply: 6)
     
787. PCR problems - Fungal DNA (reply: 6)
     
788. PCR contamination that comes and goes! - (reply: 5)
     
789. gel purification for PCR product - (reply: 5)
     
790. How long we can keep Diluted Primer? - (reply: 5)
     
791. Primer calculation - (reply: 3)
     
792. Real-time PCR....Making sense of it all? - (reply: 3)
     
793. Real-time PCR vs. Northern Blotting / mRNA stability assays... - Advice from those who have experience with mRNA stability assays, plea (reply: 2)
     
794. PCR primer resuspend method? - (reply: 9)
     
795. Testing newly designed primers prior to real time - (reply: 2)
     
796. Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
     
797. autoclave PCR tubes... - (reply: 8)
     
798. RT PCR produces nice crisp band but of wrong length :-) - (reply: 2)
     
799. multiple displacement amplification - (reply: 2)
     
800. Help needed. PCR band problem - (reply: 4)
     
801. PCR 'contamination' - (reply: 16)
     
802. 2/3Kb PCR fragment - 16S/23S (reply: 3)
     
803. About Degeneracy of a degenerate primer~~ - (reply: 2)
     
804. Where do dNTP come from? - Chemical or Biological? (reply: 12)
     
805. strange red lines in all of my agarose gels from PCR - (reply: 2)
     
806. polimorph markers in inbred mouse strains? - microsatellites or any PCR based suggestins are wellcome! (reply: 1)
     
807. PCR on PCR product - (reply: 5)
     
808. RT-PCR gene specific amplification problem - (reply: 1)
     
809. Cloning short sequences - Does PCR can help clones upto 70 bps into a protein sequence (reply: 10)
     
810. information regarding PCR using very long primers - (reply: 4)
     
811. RT-PCR: Band in no reverse transcriptase lane - (reply: 18)
     
812. Non repeatability of RT-PCR with same cDNA - (reply: 3)
     
813. what is the plasmid nomenclature: eg.what does it mean by pUC, pCR, and other ve - (reply: 13)
     
814. MSP primer design - understanding the basics - (reply: 2)
     
815. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
     
816. Bisulfite: PCR condition or primer problem? - (reply: 13)
     
817. Real-time PCR to analyze DNA methylation - (reply: 3)
     
818. I really need an answer on PCR (16s rRNA), PLS PLS PLS! - (reply: 2)
     
819. Primer concentration and standard curve slope in "Relative Standard Curve M - (reply: 1)
     
820. storage of primers - (reply: 3)
     
821. real time pcr designing software - (reply: 1)
     
822. Problem with pcr, help required - PCR product (reply: 12)
     
823. smearing in PCR product - (reply: 4)
     
824. Curious - different primer synthesis? - (reply: 4)
     
825. 2-step RT-PCR - (reply: 1)
     
826. Windows in PCR/Clean rooms..... - (reply: 8)
     
827. 'ladder bands' in PCR - (reply: 11)
     
828. help a student with a PCR process question? - (reply: 6)
     
829. Polymerase for Long PCR - (reply: 5)
     
830. too many hits on Blast for primers / microsatellite - (reply: 4)
     
831. Sequencing PCR products with biotin-labelled primer - (reply: 1)
     
832. sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
     
833. "Ligate" two overlapping pcr products with PCR fusion? - (reply: 1)
     
834. how to get DNA sequences starting from the primer - (reply: 4)
     
835. Primer orientation, forward and reverse - (reply: 4)
     
836. How to remove a few bases from my cloned insert using PCR - (reply: 3)
     
837. Bisulfite sequencing of PCR products after AIMS technique - (reply: 3)
     
838. PCR help - (reply: 1)
     
839. Help reamplifying the Fusion PCR - (reply: 3)
     
840. PCR amplification using universal 16s rDNA - it's necessary to do it (reply: 3)
     
841. primers for bisulfite-modified DNA: general questions - (reply: 6)
     
842. LM-PCR amplification - (reply: 1)
     
843. 5'-RACE PCR - (reply: 3)
     
844. RT-PCR NTC- substitute DNA with water? - or just end up with a smaller rxn volume? (reply: 1)
     
845. RT-PCR Controls (no DNA) show weird amplification - Complicated problem- urgent help needed! (reply: 4)
     
846. PCR protocols - for some antibiotic resistant strains (reply: 2)
     
847. Negative control primer for ChIP - (reply: 3)
     
848. How to quantitate the pcr product from gel picturess - (reply: 4)
     
849. inconsistent result from real time PCR - (reply: 2)
     
850. primers for promoter amplification - (reply: 1)
     
851. Cloning after amplifying with Taq polymerase? - (reply: 5)
     
852. RT-PCR primer designing - (reply: 6)
     
853. ways to get a good gene amplification - (reply: 4)
     
854. Scaling up PCR reactions - (reply: 4)
     
855. PCR with overlapping primers - (reply: 1)
     
856. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
     
857. Can any one suggest a company who can design a primer pair - dnaK PCR work (reply: 1)
     
858. cloning through PCR - (reply: 3)
     
859. PCR doubts - (reply: 7)
     
860. Homemade TaqMan PCR Mastermix? - (reply: 1)
     
861. Problem about RT-PCR for beta-actin - (reply: 2)
     
862. My primers don't work with Chip - (reply: 1)
     
863. LNA Primers design - (reply: 1)
     
864. qPCR using random primers - (reply: 4)
     
865. PCR optimization failure - (reply: 18)
     
866. designing primers for cloning - designing primers for cloning (reply: 1)
     
867. Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
     
868. no bands in PCR reaction analysis - does centrifuge time matter? (reply: 2)
     
869. PCR of methylated DNA? - (reply: 1)
     
870. PCR optimization - (reply: 5)
     
871. Verification of real-time amplification specificity - (reply: 1)
     
872. 1. ABI Power SYBR® Green primer efficiency? - (reply: 1)
     
873. pcr machine - construction (reply: 1)
     
874. Polymerase Activity Assay - (reply: 1)
     
875. GenScript- Free PCR Reagents and DNA Purification Kits! - (reply: 1)
     
876. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
     
877. Amplifying a 1700bp fragment using a mismatched primer pair - (reply: 2)
     
878. PCR inespecific band only in control - why only in the control? (reply: 2)
     
879. Long and Accurate PCR - (reply: 2)
     
880. Identification by 16S rDNA - using short primer (reply: 2)
     
881. PCR stopped working with new dNTP! - (I've tried all the usual suspects) (reply: 4)
     
882. Degenerate Primers - (reply: 12)
     
883. PCR buffer and PCR efficiency - buffer pH and salt (reply: 2)
     
884. What safety and risk assessments should i be aware of in doing PCR and gel elect - (reply: 2)
     
885. systemic way to reduce BSP PCR bias - (reply: 1)
     
886. restriction enzymes primers - (reply: 5)
     
887. Plasmid DNA Sequencing with Universal Primers - (reply: 3)
     
888. Fusion PCR - Help ragrding Fusion PCR (reply: 5)
     
889. need anealing temp for PCR - (reply: 8)
     
890. Roche produce Blunt or dA tail PCR product? - (reply: 3)
     
891. Primer Efficiency (how to change the slope) - (reply: 5)
     
892. Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
     
893. Inhibitory component of a PCR assay - (reply: 10)
     
894. Ever heard not of Prime Dimers but Primer Multimers? - (reply: 1)
     
895. PCR in 96-Well Plate - (reply: 4)
     
896. Isolation of the upstream region of a gene by PCR: a new tecnique? - (reply: 4)
     
897. Possible mutations in primers? - (reply: 4)
     
898. make PCR template blunt ended - (reply: 2)
     
899. autoclaving tubes for PCR/RT-PCR - (reply: 5)
     
900. AT rich PCR problems - anyone experienced with tetramethylammonium chlroide? (reply: 3)
     
901. Quickchange site directed mutagenesis/PCR problem - Transformation isn't working :( (reply: 8)
     
902. how would you detect a point mutation in the genome? PCR? - (reply: 3)
     
903. How many of us request for PAGE purification of primers more than 30 mers in len - PAGE purified primers (reply: 5)
     
904. KOD HiFi DNA Polymerase - (reply: 3)
     
905. PCR contamination in NoRT Control - I need advice (reply: 1)
     
906. Need to confirm PCR primers are bad - (reply: 5)
     
907. RT PCR - NO PCR PRODUCT (reply: 3)
     
908. PCR - (reply: 1)
     
909. Annealing step in PCR - (reply: 1)
     
910. Expectations for presentation of pcr data - (reply: 1)
     
911. forgot my PCR tubes 1 h at 8°c... - (reply: 7)
     
912. False negative PCR result - (reply: 5)
     
913. RT PCR strange melting curve - (reply: 3)
     
914. help with nested PCR for splice variant detection - nested PCR (reply: 3)
     
915. PCR - (reply: 5)
     
916. Ligation of half blunt PCR fragments - (reply: 4)
     
917. RNA quantification for Real Time RT-PCR - (reply: 2)
     
918. first time doing quantitative real time PCR - (reply: 1)
     
919. cloning primers, TM, and specificity - (reply: 4)
     
920. Help! Primer Dimers… - (reply: 3)
     
921. Allele Specific PCR Primer Design - (reply: 1)
     
922. RT-PCR without RNA isolation - (reply: 2)
     
923. Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? - (reply: 2)
     
924. Very low PCR primer concentrations? - (reply: 4)
     
925. Other than Taq? - for PCR after RT (reply: 3)
     
926. Inconsistent PCR results - (reply: 3)
     
927. Question about BSP primer design? - What is the complementary strand for forward primer? (reply: 3)
     
928. Multiplexing first round PCR in BSP - Any experience? (reply: 16)
     
929. Locate a lab for PCR and Gel work - (reply: 2)
     
930. PCR detectable units - PDU (reply: 2)
     
931. Primer sets for Bisulfite-modified and unmodified DNA - (reply: 6)
     
932. Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
     
933. Real time PCR of low expressing genes - Late tumor Ct (reply: 1)
     
934. no results in RACE PCR - (reply: 5)
     
935. Help with fusion PCR to piece together fragments - (reply: 5)
     
936. Very resistand band in PCR - (reply: 7)
     
937. multiplex rt pcr - (reply: 5)
     
938. Knockdown detected by western but not RT-PCR - (reply: 3)
     
939. long pCR kit - (reply: 4)
     
940. PCR Amplification from clones - (reply: 7)
     
941. Longest Amplicon Using Real-Time PCR? - (reply: 1)
     
942. Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
     
943. real time PCR with ChIP - (reply: 4)
     
944. PCR cloning problem - (reply: 6)
     
945. UV light, dangerous to purify PCR fragments? - (reply: 2)
     
946. validation of micrarray data by realtime pcr - (reply: 1)
     
947. PCR DNA IMAGES ON GEL HELP WANTED - (reply: 3)
     
948. minimum amount of rna in RT-PCR - (reply: 1)
     
949. Sss1 treatment of PCR amplicons - Potential dumb question (reply: 1)
     
950. Trying to lyse cells to get PCR template - Need something simpler than a miniprep (reply: 10)
     
951. double digested PCR product into competent cell - (reply: 2)
     
952. PCR Primer again - (reply: 1)
     
953. PCR cloning, faint band - (reply: 4)
     
954. RAPD PCR - (reply: 1)
     
955. False priming in MSP - (reply: 4)
     
956. Polymerase Choice Troubles - my primer melting temp is so low (~30 C) (reply: 4)
     
957. PCR efficiency - (reply: 1)
     
958. multiplex RT-PCR - (reply: 6)
     
959. cDNA for real-time PCR - (reply: 3)
     
960. cycling parameters for PCR - (reply: 4)
     
961. Can primers go bad and give a smear in PCR? - (reply: 12)
     
962. primer Tm - (reply: 10)
     
963. PCR product 1200bp - (reply: 3)
     
964. why no insert but can PCR insert out? - (reply: 5)
     
965. PCR cloning strategy - (reply: 4)
     
966. smear in degenerate PCR - (reply: 3)
     
967. Panomics Methylation Promoter PCR Kit - (reply: 1)
     
968. Single Codon Deletion - SOE PCR - (reply: 3)
     
969. QuickChange primers self-made - (reply: 4)
     
970. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
     
971. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
     
972. PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
     
973. Mystery PCR product at lower dilutions - (reply: 3)
     
974. PCR for checking transfection - (reply: 6)
     
975. Non specific amplification - How to check if the primers give non specific amplification or not (reply: 4)
     
976. digoxygenin-labelled primer - (reply: 3)
     
977. DNA conc for PCR - (reply: 1)
     
978. How to import GeBank files into Primer Express 2.0 - (reply: 1)
     
979. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
     
980. ChIP: I get smear after PCR? - (reply: 2)
     
981. Why my ChIP PCR results are always unrepeatable? even from the same sample. - (reply: 7)
     
982. Old PCR publication - help! - (reply: 2)
     
983. freezing of primers - (reply: 2)
     
984. Primers with RE sites - (reply: 2)
     
985. Smeared PCR product - ISSR marker screening (reply: 1)
     
986. overlap extension using PCR (3way PCR) - (reply: 1)
     
987. PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR (reply: 6)
     
988. normalization for real-time PCR - (reply: 4)
     
989. Primer dilution and calculation - (reply: 4)
     
990. what can i do with this pcr? - amplification of cDNA , with primers tm 50 and 67 (reply: 7)
     
991. Designing primer for Banana mild mosaic virus - (reply: 3)
     
992. Fusion PCR for eventual TA cloning - (reply: 2)
     
993. Ligation insert without PCR? - (reply: 6)
     
994. improve the DNA cons. in RT-PCR - (reply: 2)
     
995. attaching t7 promoter sequence to template primer - (reply: 1)
     
996. Inverse PCR problem! plz help! - (reply: 2)
     
997. southern conflicts with PCR in knock-in mouse screening - why southern positives are not the mutant we want (reply: 5)
     
998. primer for real-time and reverse transcriptase PCR - (reply: 1)
     
999. PRIMER 3 PROGRAMME - (reply: 8)
     
1000. reverse transcription PCR - (reply: 1)
      
1001. Statistical analysis of quantitative real-time PCR (qRTPCR) - Statistics on qRTPCR data (reply: 2)
      
1002. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
      
1003. primers stopped working - (reply: 3)
      
1004. RT-PCR not working - (reply: 8)
      
1005. humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
      
1006. PCR for >4kb, i got no product. - how shud i optimize my pcr (reply: 9)
      
1007. PCR on very short sequences - (reply: 3)
      
1008. Representing RT-PCR Data - (reply: 2)
      
1009. getting smear in PCR - (reply: 3)
      
1010. do you ever use PAGE to check PCR? - (reply: 3)
      
1011. Qiagen EpiTect - Kit - No PCR product after BSP, only smear! (reply: 11)
      
1012. PCR puzzle- big or small - PCR (reply: 1)
      
1013. Validating amplification efficiencies of the target and the housekeeping genes - (reply: 1)
      
1014. klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
      
1015. RT-PCR Question.. - (reply: 1)
      
1016. explaining competitive PCR - (reply: 1)
      
1017. Amplifying pathogen DNA in total DNA extractions - (reply: 2)
      
1018. Anyone ever do a molecular beacon PCR? - (reply: 1)
      
1019. optimization of cDNA template for real time PCR array - (reply: 2)
      
1020. Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
      
1021. RT-PCR kit - (reply: 3)
      
1022. Primers going bad - what's the best storage/usage conditions? (reply: 4)
      
1023. Weird PCR results from 2 different samples - (reply: 9)
      
1024. 5' Primer modification - Tm-effect ?? (reply: 1)
      
1025. condition for keeping integrity of RNA for RT-PCR - (reply: 1)
      
1026. PCR using an enzyme mix - never done this before, how do I set it up? (reply: 4)
      
1027. Different sizes of PCR products from different competent cells - (reply: 4)
      
1028. Designing PCR Primers - (reply: 3)
      
1029. sequencing - which primer should I trust? (reply: 4)
      
1030. running a gel with primers - (reply: 2)
      
1031. Help- degenerate primer design - (reply: 4)
      
1032. Long PCR on a cosmid vector - Help needed to run PCR (reply: 3)
      
1033. Does set up PCR mix days prior the amplification affects the results? - (reply: 2)
      
1034. the results of allele specific PCR is the other way around - I dont know why (reply: 1)
      
1035. Chemically Modified Taq - Taq Polymerase (reply: 3)
      
1036. Will this primer work? - (reply: 8)
      
1037. Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
      
1038. More PCR cloning problems! - (reply: 12)
      
1039. Plasmid as a PCR template - (reply: 2)
      
1040. What are the most important parameters in PCR primer design? - Tm difference, GC clamp, GC%, primer-primer complementarity, self-complementarit (reply: 9)
      
1041. role of MgCl2 and DTT in PCR - (reply: 3)
      
1042. How much PCR do I need for cloning? - (reply: 5)
      
1043. Is a Kazark sequence needed in a upper primer? - (reply: 17)
      
1044. Primers and enzymes not compatible - How can I fix this? (reply: 2)
      
1045. reampllification of pcr product - reamplification (reply: 9)
      
1046. cytokines primers - (reply: 1)
      
1047. What's the difference between using DNA and RNA polymerase for PCR - (reply: 2)
      
1048. How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
      
1049. Single primer PCR - (reply: 3)
      
1050. PCR cloning problems - (reply: 13)
      
1051. PCR amplification of large piece from Bissulfite treated DNA - (reply: 2)
      
1052. is there a loss of volume after pcr? - (reply: 5)
      
1053. Inverse PCR primers design - (reply: 4)
      
1054. Enzyme Site in reverse Primer - (reply: 3)
      
1055. BSA concentration (and dilutions) for PCR - (reply: 2)
      
1056. PCR product self life? - (reply: 2)
      
1057. help as to why there are no bands when running my PCR product - (reply: 3)
      
1058. general pcr/primer question - (reply: 1)
      
1059. Problem on PCR from cDNA - (reply: 3)
      
1060. dephosphorylated vector and PCR insert - (reply: 7)
      
1061. 2 PCR product to be inserted into 1 expression vector - (reply: 2)
      
1062. Need a FRET PCR protocol - (reply: 1)
      
1063. size of PCR product in PAGE - (reply: 5)
      
1064. for the PCR primer what is better the hairpin or the self annealing? - (reply: 9)
      
1065. RT-PCR Troubles.. - (reply: 3)
      
1066. blunt end ligation after inverse PCR - (reply: 5)
      
1067. primers in PCR - primer removed or not (reply: 5)
      
1068. PCR'ing a high GC gene - (reply: 1)
      
1069. Is this kind of primer OK? - (reply: 2)
      
1070. pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want??? (reply: 5)
      
1071. Real Time PCR : Bacterial gene quantification, can it be done? - (reply: 2)
      
1072. PCR problem with CGG repeats - (reply: 4)
      
1073. Overloading a PCR? - Is this possible? (reply: 3)
      
1074. different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
      
1075. RNA in cDNA - Can I use the same PCR primers for the production of cDNA? (reply: 3)
      
1076. Primers for BS treated DNA - (reply: 5)
      
1077. Primer Design software - (reply: 8)
      
1078. Restriction digestion of PCR product - (reply: 3)
      
1079. concentration of primers - (reply: 5)
      
1080. Problems with PCR for GATEWAY - I'd get rid of 100-150bp unspecific band!!! (reply: 5)
      
1081. Primer design - (reply: 4)
      
1082. Pfx polymerase for site-directed mutagenesis - (reply: 2)
      
1083. PCR product as template in another PCR - going crazy (reply: 5)
      
1084. Nested polymerase reaction - why to do this nested PCR? (reply: 1)
      
1085. Tac polymerase denatures at room temp why? - PCR-tac polymerase (reply: 4)
      
1086. RT-PCR product dilution and electrophoresis - (reply: 2)
      
1087. melting temperature in real time PCR - (reply: 1)
      
1088. forward & reverse primers - (reply: 3)
      
1089. Fusion protein questions - Botched deletion primer (reply: 2)
      
1090. How to calculate DNA concentration after PCR manually - not using spectrophotometry or electrophoresis (reply: 6)
      
1091. Real-Time PCR Question - (reply: 1)
      
1092. Taq Polymerase storage conditions - (reply: 1)
      
1093. PCR reaction efficiency problem - (reply: 2)
      
1094. pGEM v pTOPO PCR products for sequencing.... Help! - (reply: 3)
      
1095. DNase treatment of RNA prior to RT-PCR - (reply: 4)
      
1096. role of ammonium sulphate in PCR buffer - (reply: 2)
      
1097. Direct sequencing on BSP PCR products: problems with A and T stretches - (reply: 3)
      
1098. Primers & probes of Applied Biosystems for real time - (reply: 2)
      
1099. Primer walking - (reply: 2)
      
1100. What is the optimum amount ot bases that can be deleted by PCR? - (reply: 5)
      
1101. When I change Taq polymerase - (reply: 1)
      
1102. Can I PCR a gene of 2KB? - (reply: 3)
      
1103. Methyl Primer Express from ABI - (reply: 2)
      
1104. When I chage Taq polymerase - (reply: 2)
      
1105. PCR Optimization question - (reply: 4)
      
1106. Target Amplicon Length for real-time PCR - (reply: 4)
      
1107. Real-Time PCR/Melting Curve Question - (reply: 3)
      
1108. Primer Optimiztion - can this be done in water (reply: 8)
      
1109. DNA free RNA for PCR - (reply: 6)
      
1110. question for the PCR to cloning construct - (reply: 9)
      
1111. RT PCR for larger amplicon - (reply: 2)
      
1112. Large fragments from PCR. - problems getting 3.5kb from genomic DNA (reply: 4)
      
1113. design primer - (reply: 5)
      
1114. Mycoplasma PCR detection in media/FBS - (reply: 6)
      
1115. RT-PCR on 3kb fragment of viral RNA - (reply: 2)
      
1116. increasing sensitivity and reducing primer dimmer - (reply: 11)
      
1117. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
      
1118. Real-Time PCR Amplicon Question - (reply: 2)
      
1119. Melting Curve/Sample analysis Question (PCR) - (reply: 3)
      
1120. PCR reagents contaminated w/ bact - still ok for fungi? - (reply: 2)
      
1121. PCR a 100bp fragment - (reply: 3)
      
1122. ppt DNA directly from a digestion/ pcr - (reply: 2)
      
1123. Can DNA polimerase amplify primers itself? - need help please!! (reply: 7)
      
1124. primer design questions for confused - (reply: 2)
      
1125. Silly Question about RT-PCR/Primer design - (reply: 2)
      
1126. Anyone use phusion HF DNA polymerase? - urgent!!!! (reply: 6)
      
1127. Can you flag-tag your sequence by PCR? - cloning shortcut (reply: 7)
      
1128. How excess template can inhibit the PCR reaction? - (reply: 1)
      
1129. how can I carry out this PCR profile - (reply: 4)
      
1130. Real Time PCR and Gene expression - (reply: 1)
      
1131. WHERE CAN I BUY A COLD BLOCK FOR PCR? - (reply: 1)
      
1132. PCR enzyme activity-can you do 50 cycles - (reply: 2)
      
1133. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
      
1134. REAL TIME PCR QUESTION - (reply: 3)
      
1135. miniprep DNA v PCR product, for sequencing - (reply: 4)
      
1136. Which primer concentration for RT with very low RNA amounts? - (reply: 1)
      
1137. primer concentration - (reply: 7)
      
1138. Strange problems with B-actin amplification - (reply: 1)
      
1139. loading control of RT-PCR - (reply: 3)
      
1140. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
      
1141. no PCR product from genomic DNA - (reply: 7)
      
1142. restriction site in primers urgent - (reply: 1)
      
1143. RT-PCR primer design: Over introns and exons? - (reply: 2)
      
1144. Preparation of template for PCR - (reply: 7)
      
1145. Problem with colony PCR - (reply: 8)
      
1146. primer design problems - (reply: 4)
      
1147. Gel purification of PCR products - (reply: 2)
      
1148. Weird PCR result - (reply: 6)
      
1149. INVERSE PCR - (reply: 4)
      
1150. problems with PCR from chIP material - double band PCR from chIP material (reply: 2)
      
1151. How to get rid of longer fragment in PCR? - (reply: 1)
      
1152. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
      
1153. Which primers are better for colony PCR? - (reply: 4)
      
1154. pcr yield and primer concentration - (reply: 5)
      
1155. non-specific amplification without desired fragment - (reply: 1)
      
1156. PCR Setup stopped working - (reply: 14)
      
1157. ligation product- direct pcr - (reply: 2)
      
1158. is amplification directly from my ligation reaction possible? - (reply: 1)
      
1159. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
      
1160. Primer design with restriction sites - (reply: 3)
      
1161. Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
      
1162. Is a 100bp PCR product more prone to give non-specific signals in a hybridisatio - (reply: 1)
      
1163. PCR band for water but no contamination, Digestion not working - (reply: 10)
      
1164. help PCR of GC rich templates - (reply: 3)
      
1165. Control PCR in Ligation - Genomic DNA + linker ligation (reply: 1)
      
1166. I am so disappointed that I failed in PCR again and again - (reply: 4)
      
1167. Why there is use of only a single primer in case RAPD amplification - (reply: 1)
      
1168. differences between oligo and random primers - (reply: 2)
      
1169. overlap PCR - (reply: 5)
      
1170. My PCR product is 100bp (approx). What result is more reliable-gel or hybridisat - (reply: 5)
      
1171. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
      
1172. Why is it difficult to PCR taget gene in rice successfully ? - (reply: 1)
      
1173. dNTP calculation - (reply: 3)
      
1174. PCR contamination - (reply: 2)
      
1175. RT-PCR primers - (reply: 3)
      
1176. Effect of Temperature on PCR - (reply: 3)
      
1177. How does isopropanol inhibit PCR? - (reply: 4)
      
1178. Difficulties getting a 4 kb PCR product - (reply: 5)
      
1179. Yet another PCR problem/question - (reply: 11)
      
1180. PCR product multiple bands - (reply: 3)
      
1181. PCR BLOck contamination? - better anti-contaminant PCR - tube? (reply: 15)
      
1182. Any primer design programs...? - (reply: 8)
      
1183. Problem SNP that genotypes differently using different primers? - (reply: 2)
      
1184. Which result is correct-Western or RT-PCR - (reply: 5)
      
1185. Transforming after digesting PCR product - (reply: 6)
      
1186. Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
      
1187. Standard PCR banding? - multiple bands instead of one (reply: 4)
      
1188. PCR problems, huge artifacts, no product - involves 16S primers (reply: 8)
      
1189. primer : shifting peak - (reply: 4)
      
1190. rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
      
1191. about RAPD PCR of plant genomes? - (reply: 4)
      
1192. Touch down PCR - (reply: 8)
      
1193. PCR ...introduction - (reply: 16)
      
1194. RealTime PCR of microRNAs - (reply: 9)
      
1195. RT-PCR of apoptosis related genes - (reply: 2)
      
1196. Primer design for BSP - (reply: 12)
      
1197. gDNA salvage for PCR from mounted tissue sections - Is it possible? (reply: 1)
      
1198. Designing primers and hybridization probes - (reply: 1)
      
1199. HMW band in well after bisulfite PCR - (reply: 1)
      
1200. Junk/dimers on left of single product peak - (reply: 3)
      
1201. Random Primers vs Oligo(dT) primers? - (reply: 3)
      
1202. gel purification of PCR product - (reply: 1)
      
1203. Is all lab bacterial strains have t7 RNA polymerase gene... - (reply: 4)
      
1204. RT PCR Primer - Software (reply: 2)
      
1205. ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
      
1206. Possible to clone when you have only 20ng PCR product? - (reply: 10)
      
1207. 5' phosphorylated primers - (reply: 1)
      
1208. Increased signal in IP after peptide addition? - Immunoprecipitation signal amplification (reply: 2)
      
1209. Methylation specific primer designing - (reply: 1)
      
1210. How to PCR a 100bp fragment - (reply: 4)
      
1211. primer design with intentional mismatch - (reply: 3)
      
1212. Problems with PCR following bisulfite treatment - (reply: 11)
      
1213. Amplifying a 6.5 kb cDNA - (reply: 3)
      
1214. pBluescript cloning of PCR products - (reply: 1)
      
1215. Ligating blunt adapter to PCR product - (reply: 7)
      
1216. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
      
1217. RT-PCR problems on mouse ear RNA - (reply: 1)
      
1218. vector PCR problem - stuck in experiments... (reply: 2)
      
1219. promoter methylation PCR kit from Panomics - (reply: 2)
      
1220. please tell me more about primers with restriction site! - (reply: 4)
      
1221. PCR 3kb fragment - (reply: 3)
      
1222. Problem in Patch PCR for Site-Directed Mutagenesis - (reply: 5)
      
1223. RT-PCR primer design - (reply: 1)
      
1224. transgenic mouse - identification by PCR (reply: 2)
      
1225. Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
      
1226. pcr detection of pathogens - (reply: 3)
      
1227. how to check primers - (reply: 4)
      
1228. Recombinant PCR - (reply: 5)
      
1229. PCR after in vitro transcription and translation - (reply: 2)
      
1230. standard curve and differentiation asays - Real Time PCR (reply: 2)
      
1231. [PCR] Which substances cause inhinbition of Taq? - (reply: 5)
      
1232. [PCR] Different amplification-efficiency of varying DNA - (reply: 1)
      
1233. troubles in PCR amplifying long target - (reply: 4)
      
1234. allele-specific amplification - (reply: 1)
      
1235. PCR clone a gene with known 3' end only - (reply: 4)
      
1236. Double bands in PCR - WHY? - (reply: 5)
      
1237. PCR Cloning - (reply: 3)
      
1238. Long range PCR - What enzymes do you use? - (reply: 6)
      
1239. Bis. PCR not working. MSP is fine - (reply: 1)
      
1240. sear in PCR no bands - (reply: 3)
      
1241. How to design primer for unknown DNA sequence? - (reply: 9)
      
1242. Few colonies with cloning of PCR product after digestion - (reply: 7)
      
1243. TM for primers with restriction secuence tail. - Suggestion (reply: 3)
      
1244. can primers age? - (reply: 1)
      
1245. How to determine PCR Annealing temperature for primers with different Tm - (reply: 7)
      
1246. No PCR Product after Bisulfite Modification - (reply: 5)
      
1247. poor random priming for Northern Blot probes - (reply: 1)
      
1248. PCR with 75 mer forward primer - (reply: 4)
      
1249. strange RT-PCR expression - (reply: 1)
      
1250. half life of primers - (reply: 2)
      
1251. PCR band disappears in agarose gel! - Agarose gel electrophoresis (reply: 4)
      
1252. competition of multiple PCR products - (reply: 1)
      
1253. MOLECULAR TEST WITH !JUST PCR AND GEL! FOR A STARTER - PLEASE GIVE ME SUGGESTIONS (reply: 3)
      
1254. PCR from Genomic DNA - (reply: 3)
      
1255. Problem with the PCR Product and Extraction - (reply: 2)
      
1256. about the primers in MSP - (reply: 5)
      
1257. SYBR green problem.. - real time PCR SYBR (reply: 2)
      
1258. ARMS PCR - (reply: 3)
      
1259. Why do a nested PCR? (HIV virus) - (reply: 4)
      
1260. cloning problem with pcr and pegfp vector - (reply: 9)
      
1261. Should I clone my PCR product even though it is invisible on gel? - (reply: 5)
      
1262. What annealing temperature is better for my primers? - (reply: 7)
      
1263. primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
      
1264. primer concentration (calculation problem) - (reply: 5)
      
1265. PCR, labelled vs unlabelled primers - (reply: 4)
      
1266. Problem with cloning p53-suggestions for RT-PCR - (reply: 1)
      
1267. How to add restriction enzyme sites to the primer - (reply: 6)
      
1268. PCR: same premix, same everything, different results... - (reply: 21)
      
1269. Using pentadecamer primers for reverse transcription - Better than hexamers, oligo d(T)? (reply: 7)
      
1270. How to confirm a 80bp PCr fragment? - (reply: 5)
      
1271. pcr grape vine - (reply: 1)
      
1272. RT-PCR control for Vitis vinifera - (reply: 2)
      
1273. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
      
1274. Problem PCR amplifying gene promoter regions - (reply: 4)
      
1275. Problem amplifying 8kb bacterial genomic DNA - (reply: 2)
      
1276. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
      
1277. syber green with real time PCR - (reply: 17)
      
1278. primer:cross-dimer - How to remove the cross dimer? - (reply: 3)
      
1279. Got different size PCR products from chIP - (reply: 7)
      
1280. PCR trouble.....help! - (reply: 16)
      
1281. Re-amplify PCR product by realtime PCR? - (reply: 13)
      
1282. My PCR's are killing me! - nothing is working!! (reply: 9)
      
1283. pcr product transfection help - (reply: 3)
      
1284. PCR almost like false positive problem! - How can I get rid of 5bp band near mine !!!!!!! (reply: 2)
      
1285. Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
      
1286. dNTP dilution for PCR - (reply: 4)
      
1287. housekeeping gene in real-time PCR - (reply: 2)
      
1288. can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
      
1289. Constant amplification in PCR negative control - (reply: 2)
      
1290. alternative to dmso in pcr - (reply: 3)
      
1291. Hemin amplifying ECL signal?!? - (reply: 5)
      
1292. The weirdest primer ever - (reply: 7)
      
1293. oligo(dT) plus random primers in RT - (reply: 4)
      
1294. Trouble with real-time PCR standard curve - (reply: 4)
      
1295. PCR inhibitors - PCR inhibitors (reply: 1)
      
1296. no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
      
1297. primer calculation - concentration (reply: 2)
      
1298. What happens to PRIMERS at 72 C ? - (reply: 5)
      
1299. Colony PCR was carried out following lysis of cells with Igepal CA - 630 .. - But how? (reply: 2)
      
1300. PCR cleanup - Quick question (reply: 1)
      
1301. 0,2uM is 100ng of a 18-mer primer - (reply: 8)
      
1302. No PCR product detected in agraose gel - (reply: 7)
      
1303. PCR problem - 1.8 kb fragment amplification (reply: 3)
      
1304. 2 melting peaks in real-time PCR - (reply: 1)
      
1305. PCR amp. - sequencing problem - (reply: 3)
      
1306. visualizing unknown DNA with linkers and PCR - (reply: 2)
      
1307. Difference between MgCl2 and MgSO4 in PCR? - (reply: 4)
      
1308. DNA fingerprinting - Is it necessary to use DNA polymerase with proof-read function in PCR? (reply: 2)
      
1309. Melting Curve giving flutating Ct values - Advice on primer design? (reply: 9)
      
1310. checking colonies by vector primers.... - (reply: 7)
      
1311. Pfu polymerase from promega - (reply: 4)
      
1312. Does 2-mercaptoethanol inhibit PCR reactions? - anyone know? (reply: 7)
      
1313. PCR: annealing temperature - (reply: 3)
      
1314. Nested PCR troubleshooting - no secondary product - (reply: 5)
      
1315. Direct BSP using tag-modified primers - (reply: 2)
      
1316. adding restriction sites to degenerate primers - (reply: 9)
      
1317. PCR: multiplex ok, single reactions not - microsatellite analysis (reply: 2)
      
1318. Two to use PCR to get domain deletion within a protein? - (reply: 2)
      
1319. Added DNTP solution to DNA by mistake - (reply: 3)
      
1320. Standard vs High fidelity polymerase, please help - No results with high fidelity (reply: 5)
      
1321. Mutagenesis using 4 sets of primer - (reply: 6)
      
1322. PCR on a GC rich region - PCR on a GC rich region (reply: 4)
      
1323. Relative Quantification in real-time RT-PCR - (reply: 5)
      
1324. PCR amplification of Community DNA - (reply: 6)
      
1325. library titering and amplification - (reply: 1)
      
1326. Unwanted band seen along with my pcr product. - regarding PCR product with unwanted band formation during electrophoresis (reply: 3)
      
1327. Wierd amplification pattern. - (reply: 8)
      
1328. cloning primer and tag questions - first try (reply: 2)
      
1329. nested PCR: purification? - (reply: 1)
      
1330. PCR problems with 1,7kb fragment - (reply: 8)
      
1331. Question about bisulfite PCR - (reply: 2)
      
1332. reuse PCR gel - (reply: 2)
      
1333. PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
      
1334. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
      
1335. pro-PCR with only one primer... - to check directionality. (reply: 4)
      
1336. Two design programs give very different primer Tm values - RT-PCR (reply: 5)
      
1337. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
      
1338. Problem of Smear - Smearing of PCR product (reply: 7)
      
1339. Inconsistent results from replicates in Taqman real-time PCR - (reply: 26)
      
1340. cDNA synthesis primer - (reply: 3)
      
1341. Amplification of phage library - (reply: 3)
      
1342. gsamsa - Gene array vs PCR (reply: 4)
      
1343. Primer with tag: Which is the real concentration? - (reply: 3)
      
1344. Any one can tell me what happens to my RT-PCR? - Urgent !RT-PCR really kills me! (reply: 5)
      
1345. PCR Trouble - (reply: 5)
      
1346. PCR of herbarium specimes - PCR of Caesalpinia herbarium specimes (reply: 1)
      
1347. Problems (unspecific products) with PCR to determine the size of cDNA inserts - (reply: 7)
      
1348. pcr product transfection - (reply: 8)
      
1349. antigen primers - (reply: 1)
      
1350. restriction digestion of PCR product? - (reply: 10)
      
1351. How to design PCR primers in ChIP assay - (reply: 2)
      
1352. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
      
1353. Annealing vs melting Ts for real time PCR primers - (reply: 1)
      
1354. Taq polymerase vs HotstartTaq polymerase - (reply: 4)
      
1355. what hybridization temp in pcr - (reply: 5)
      
1356. relative quantification, primer pair problem - (reply: 1)
      
1357. Pfx PCR problem - (reply: 6)
      
1358. Question about PCR reaction condition - (reply: 3)
      
1359. RNA Amplification - (reply: 2)
      
1360. PCR Internal Controls - (reply: 2)
      
1361. Calculating Tm for bisulfite primers? - (reply: 3)
      
1362. smear in PCR - No consistancy in amplification (reply: 3)
      
1363. real-time PCR for quantitation of MSP - (reply: 3)
      
1364. Designing species specific primers for E.coli - please help? (reply: 3)
      
1365. DMSO Contamination in PCR - (reply: 3)
      
1366. Chloramphenicol amplification - (reply: 5)
      
1367. Fusion/overlap PCR troubleshooting - (reply: 3)
      
1368. Screening a Gene Library - I want to use colony PCR, but How (reply: 4)
      
1369. how could one avoid amplification of a very high non-specific band from PCR - (reply: 3)
      
1370. Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
      
1371. Using primers meant for DIG probe as PCR diagnostic - (reply: 3)
      
1372. Need for Nested PCR - Primers Designed with MethPrimer - (reply: 6)
      
1373. Real time PCR for gene family - (reply: 3)
      
1374. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
      
1375. Do I have to make pcr master mix fresh every time - (reply: 1)
      
1376. 2nd round of amplification issues - (reply: 1)
      
1377. Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
      
1378. Smearing of the PCR Products - (reply: 10)
      
1379. Primer for 5' RACE - (reply: 2)
      
1380. Stick T7 on custom primer for sequencing? - (reply: 2)
      
1381. RNA polymerase? - (reply: 5)
      
1382. SMEAR IN POSITIVE CONTROL IN LONG PCR - (reply: 3)
      
1383. To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
      
1384. Cloning PCR product - Any ideas? (reply: 3)
      
1385. ABI have released MethylPrimer Express! - A new primer design program for DNA methylation....For Free!!! (reply: 45)
      
1386. MSP primer design help - (reply: 5)
      
1387. PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
      
1388. Primers for nested-PCR - (reply: 1)
      
1389. PCR primers with high Tm - (reply: 6)
      
1390. no product after PCR - (reply: 5)
      
1391. how to choose best annealing temp. for primers? - (reply: 6)
      
1392. Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
      
1393. PCR using a long (100nucleotides) and a short (20 nucleotides) primer. Problem: - (reply: 7)
      
1394. making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
      
1395. PCR quickchange with high Tm oligos - help needed (reply: 2)
      
1396. BSP and MSP Primers - (reply: 2)
      
1397. PCR mixing - First tubes generally work, last ones generally don't. (reply: 3)
      
1398. PCR question, need help please! - (reply: 5)
      
1399. Storage of PCR product? - (reply: 1)
      
1400. Bisulfite sequencing primer - (reply: 4)
      
1401. MSP Primer Sets (Herman Papers) - (reply: 1)
      
1402. specific loci not present depending on DNA extraction method? - DNA extraction and succesful PCR (reply: 1)
      
1403. Can pfu Polymerase make a blunt end? - (reply: 3)
      
1404. Methods for ligating fragments to form dimers.. - (reply: 1)
      
1405. real time PCR machine choice - (reply: 1)
      
1406. Must all BSP be nested PCR? - (reply: 4)
      
1407. random primers for qPCR - (reply: 3)
      
1408. QPCR, where to start? - optimizing primer and template (reply: 5)
      
1409. difficulty in Primer walking - (reply: 2)
      
1410. about nested PCR - (reply: 2)
      
1411. PCR primers Tm higher than extension T - (reply: 8)
      
1412. Primer for sequencing - need help!!!!!!!!!! (reply: 6)
      
1413. Primer Design - (reply: 2)
      
1414. Primer design invert pcr - (reply: 5)
      
1415. fetal mistake in my hot start PCR - (reply: 3)
      
1416. Standard PCR - (reply: 3)
      
1417. cloning two fragments with deletion in between - PCR primer design (reply: 3)
      
1418. Left my PCR supplies out overnight. Are they all ruined? - (reply: 4)
      
1419. PCR not works time to time - (reply: 2)
      
1420. faster way to identify RNA virus other than RT-PCR? - (reply: 2)
      
1421. RT-PCR a GC Rich (68%) gene (2200bp) - (reply: 6)
      
1422. Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
      
1423. Two bands on RT-PCR product - (reply: 8)
      
1424. designing primers for Real Time - (reply: 1)
      
1425. RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge (reply: 3)
      
1426. Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
      
1427. Duplicate PCR: specific bands but different sizes. - (reply: 2)
      
1428. Real time PCR late amplification - (reply: 6)
      
1429. 4 deg Celsius after PCR - (reply: 1)
      
1430. PCR anealing temperature question - (reply: 12)
      
1431. PCR buffers for Taq and Pfu polymerase - (reply: 6)
      
1432. Taq and Pfu polymerase give different amplification - (reply: 8)
      
1433. Cloning a PCR product after digestion - (reply: 7)
      
1434. Methods for primer reconstitution - (reply: 23)
      
1435. Smearing in "Overlap Extension PCR" - (reply: 1)
      
1436. Help on PCR purification - (reply: 2)
      
1437. ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (reply: 1)
      
1438. BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
      
1439. differences between PCR and Quick Change PCR - what are thedifferences between PCR and Quick Change PCR? It's urgent (reply: 2)
      
1440. Troubleshooting PCR contamination - (reply: 13)
      
1441. ribosomal binding for pcr product cloning - urgent!!! (reply: 2)
      
1442. no result with pcr. is it the primer?/ - no amplification in pcr even after starting from the beginning (reply: 6)
      
1443. Digesting PCR products - (reply: 2)
      
1444. PCR result: Nothing! - Urgent! (reply: 2)
      
1445. RT-PCR mouse RNA released from complement-lysed cells - Goal: to whether RNA is of nuclear or mitochondrial origin. Planning to PCR ampl (reply: 2)
      
1446. PCR large fragments - (reply: 2)
      
1447. dimers of dna (not primers) following digest or pcr? - (reply: 11)
      
1448. can we add the terminaton codon in the 3' primer? - (reply: 1)
      
1449. Question about Realtime PCR primer concentration.... - (reply: 5)
      
1450. PCR probes for Southern blot - (reply: 1)
      
1451. Smear in PCR - (reply: 5)
      
1452. Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
      
1453. PCR amplification - (reply: 2)
      
1454. designing primer for methylation - (reply: 21)
      
1455. plasmid amplification - exons and primers (reply: 1)
      
1456. Primer dimers?Contaminations? - Unexpected bands! (reply: 5)
      
1457. reverse primer design with restriction site - (reply: 6)
      
1458. Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
      
1459. PCR dry mixture - (reply: 3)
      
1460. Use PCR products to determine efficiency of amplification? - (reply: 7)
      
1461. MSP PCR - (reply: 2)
      
1462. Band in negative PCR control - (reply: 25)
      
1463. Different RNA sources, Different PCR optimal condition? - (reply: 5)
      
1464. question in 5' RACE and primer extesion - (reply: 8)
      
1465. PCR contamination - does autoclaving help? - (reply: 7)
      
1466. Primer concentration ? - (reply: 2)
      
1467. PCR and DMSO - (reply: 2)
      
1468. PCR my cDNA & got nothing - (reply: 2)
      
1469. Dimer of PCR products? - (reply: 2)
      
1470. PCR product dimer (not primer dimer!) - PCR (reply: 2)
      
1471. Taq/PFU polymerase A overhang - bad for blunt ending? (reply: 3)
      
1472. Degradation of PCR product during purfication - Purification of PCR product for cloning (reply: 2)
      
1473. BAC clone amplification - (reply: 1)
      
1474. Nonspecific PCR amplification? - (reply: 2)
      
1475. QIAquick PCR for preparing for restriction digestion - very low concentrations (reply: 4)
      
1476. Plasmid PCR - (reply: 5)
      
1477. pcr products wont give me colonies - (reply: 3)
      
1478. Nested allele-specific PCR - (reply: 1)
      
1479. No PCR product for genotyping - (reply: 2)
      
1480. problems in subcloning PCR products for TA cloning - (reply: 4)
      
1481. Q-PCR questions: Primer design and melting curve - (reply: 13)
      
1482. no product for promoter genomic PCR - (reply: 3)
      
1483. MgCl vs MgSO4 for PCR - (reply: 2)
      
1484. from which strand to derive BSP primers? - (reply: 5)
      
1485. Validating microarray results - Real-time RT-PCR (reply: 1)
      
1486. SMear instead of PCR product - (reply: 3)
      
1487. PCR - free primer designation program (reply: 2)
      
1488. Best product size for real-time RT-PCR - (reply: 4)
      
1489. No success with PCR cloning of a gene into expression vector - (reply: 5)
      
1490. what is the principle of gradient PCR? - How does it work (reply: 2)
      
1491. Primer Annealing for PCR - (reply: 4)
      
1492. Why is my smaller band too faint? Genomic PCR not working! - Just trying to genotype some mice... (reply: 2)
      
1493. cDNA library screening with 5' specific primer - (reply: 1)
      
1494. Removal of PCR inhibitor - (reply: 3)
      
1495. Site-Directed Mutagenesis- Doubt - Overlapping primers (reply: 3)
      
1496. Both the PCR primers in a vial. - Did anybody mix the diluted primers in single tube for use? (reply: 7)
      
1497. PCR mix and DEPC water - (reply: 2)
      
1498. Concentrating PCR product - (reply: 9)
      
1499. PCR inhibitors - What are common pcr inhibitors (reply: 9)
      
1500. How to design primer for sequencing unknown DNA - (reply: 5)
      
1501. PCR contaminant - (reply: 4)
      
1502. PCR mix and DEPC water - (reply: 2)
      
1503. PCR - forward, reverse primers - is the reverse primer the "reverse complement" of the sense strand (reply: 2)
      
1504. need helps on probe design--human ip10 primers and probe. - (reply: 2)
      
1505. another PCR question - (reply: 12)
      
1506. PCR - (reply: 8)
      
1507. PCR doesn't work! - Falling in an empty gel "space" (reply: 2)
      
1508. Success with Direct Sequening of Bisulfite Pcr Products - (reply: 47)
      
1509. PCR contamination false positives - (reply: 6)
      
1510. how to determin the Annealing Temp of ristriction sites modified primer? - help me ! (reply: 26)
      
1511. Sequencing Primers - (reply: 4)
      
1512. two bands of one gene in PCR - (reply: 8)
      
1513. 5' RACE - gene specific primer - (reply: 1)
      
1514. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
      
1515. Viral RT PCR - (reply: 2)
      
1516. About PCR primers' concentration - (reply: 2)
      
1517. Reliability of PCR? - (reply: 4)
      
1518. Multiple unknown amplification products - (reply: 2)
      
1519. PCR in 25 uL is it sufficient? - (reply: 6)
      
1520. Qiagen Gel Extraction of PCR product - (reply: 7)
      
1521. Primer design with incomplete sequence - (reply: 2)
      
1522. Problem with lids to 8-strip .2 ml PCR tubes - (reply: 3)
      
1523. Annealing temperatures for long primers? - PCR reaction (reply: 6)
      
1524. Measuring the amount of PCR product - (reply: 9)
      
1525. PCR primers from amino acids? - (reply: 1)
      
1526. difficult PCR - (reply: 1)
      
1527. Some Questions about PCR - ... based von Wikipedia article (reply: 1)
      
1528. Low PCR product for antibody gene - (reply: 4)
      
1529. chronic PCR contamination - (reply: 11)
      
1530. Colony PCR positive, miniprep negative? - (reply: 9)
      
1531. TA cloning: if PCR product is not freshly prepared - (reply: 2)
      
1532. RT-PCR machine/setup problems - (reply: 2)
      
1533. Minimum amount of RNA for real-time PCR - (reply: 4)
      
1534. Primer dimers - (reply: 1)
      
1535. RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
      
1536. troubles with human genomic DNA amplification - (reply: 1)
      
1537. PCR vs biochemical tests - which one is more reliable? (reply: 6)
      
1538. RT-PCR question - genomic DNA amplified with RNA (reply: 9)
      
1539. DNA amplification for ChIP-on-chip - (reply: 1)
      
1540. Preserve PCR mix - (reply: 3)
      
1541. genomic dna in real-time pcr - (reply: 3)
      
1542. problem concerning PCR Test after disruption and yeastgenome DB - (reply: 6)
      
1543. More about PCR dimers! - (reply: 1)
      
1544. primers design for mutagenesis of two nearby sites - mutations (reply: 2)
      
1545. HELP! I have a PCR amplifing problem - PCR amplifing problem (reply: 4)
      
1546. Asymmetric PCR - (reply: 2)
      
1547. Purify integrating PCR product before transforming yeast? - (reply: 1)
      
1548. PCR of long fragment(approx 8 kb) - (reply: 3)
      
1549. What does the term "smear" mean in PCR ? - (reply: 3)
      
1550. PCR..HELP - band in no template control (reply: 1)
      
1551. RT-PCR Control Reactions - Need suggestions! - (reply: 1)
      
1552. Troubleshooting PCR smear - Urgent,Need help! (reply: 4)
      
1553. Reamplifying my PCR product - (reply: 7)
      
1554. [urgent help] Band missing in PCR - (reply: 5)
      
1555. DNase I Amplification grade and DNase I for RNA treatment - (reply: 1)
      
1556. Multiplex PCR - (reply: 4)
      
1557. design spesific primer of avr, hrp, toxin and falgella gene - (reply: 3)
      
1558. Smearing in nested pcr product - (reply: 2)
      
1559. no pcr product with pfu/pwo but with taq - (reply: 3)
      
1560. Intensive primer dimers - (reply: 2)
      
1561. pcr contamination - (reply: 3)
      
1562. PCR using different primers to generate same DNA sequence - explanation please :) (reply: 2)
      
1563. PCR problem: long product (4 kb) from a cDNA library - (reply: 2)
      
1564. PCR no longer works - no pcr product? (reply: 3)
      
1565. quantifying cDNA for Real Time PCR - (reply: 3)
      
1566. What should I do for more samples need to be tested by real-time PCR? - (reply: 1)
      
1567. no band after long-distance PCR - (reply: 3)
      
1568. Laddering effect in PCR blank control - (reply: 4)
      
1569. PCR is positive with one set of primers, negative with another. - (reply: 4)
      
1570. Do you synthsize your own oligo(dT) primer? - (reply: 2)
      
1571. Can't get MSP PCR products at all after treatment! - Help with MSP using Chemicon Fast DNA Mod Kit (reply: 1)
      
1572. PCR on Genomic DNA - (reply: 2)
      
1573. Trouble with primers of BSP - (reply: 3)
      
1574. Could higher ammount of taq be inhibitory for overall PCR reaction? - (reply: 2)
      
1575. Touchdown PCR for MSP? - (reply: 4)
      
1576. genotyping mice-no pcr product - (reply: 3)
      
1577. PCR smears are getting me crazy! - (reply: 1)
      
1578. Nested PCR - no amplicon - (reply: 1)
      
1579. PCR from pit soil type DNA sample - (reply: 3)
      
1580. PCR - (reply: 3)
      
1581. degenerate primer design - (reply: 4)
      
1582. Volume of Real time PCR mastermix and reaction - (reply: 4)
      
1583. Decreasing Amplification Curve for Standards - (reply: 2)
      
1584. Weird duplex PCR results - (reply: 5)
      
1585. Home-made Sybr green mix for real-time PCR - (reply: 1)
      
1586. PCR - (reply: 4)
      
1587. PCR buffers - can I use MgCl2 alone (reply: 4)
      
1588. potassium acetate in primer solution - I made a mess but need to recover (reply: 6)
      
1589. RNA storing/RT PCR using oligo dT - (reply: 5)
      
1590. cloning/amplifying attenuate RNA transcripts - (reply: 6)
      
1591. PCR with attB not working - (reply: 1)
      
1592. Nested PCR problems - (reply: 1)
      
1593. Very, VERY weird PCR results! - (reply: 4)
      
1594. confirmation of my pcr product - (reply: 2)
      
1595. What is the advantage of nest PCR in methylation analysis - (reply: 1)
      
1596. 16S rRNA primers for E. coli - (reply: 2)
      
1597. Insert size longer than expected on PCR - (reply: 3)
      
1598. About PCR smear - Anyone can help me? (reply: 4)
      
1599. preferential amplification among transcripts - (reply: 1)
      
1600. primer design - how to design primers (reply: 2)
      
1601. Should I dilute cDNA for real-time PCR? - (reply: 3)
      
1602. Real-time PCR problems - RT_PCR (reply: 7)
      
1603. PCR cloning problem - No colonies - (reply: 10)
      
1604. HIV RT PCR KIT - (reply: 1)
      
1605. strange contamination in real-time PCR in negative control? - (reply: 1)
      
1606. Real time PCR - no product - (reply: 1)
      
1607. Impact of temperature and time increment during PCR - (reply: 3)
      
1608. Multiplex Primers - Primer primer interaction (reply: 1)
      
1609. dNTP degradation - (reply: 4)
      
1610. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
      
1611. inconsistent PCR results - (reply: 2)
      
1612. PCR smear in samples and negative control - (reply: 5)
      
1613. pcr sequencing - (reply: 2)
      
1614. How should I check my colonies? - PCR check is not working...:( (reply: 5)
      
1615. RT-PCR of an intron - possible plateau effect? (reply: 2)
      
1616. question about mutagenic PCR. - (reply: 2)
      
1617. Why primers for bisulfite converted DNA amplify unconverted DNA? - (reply: 3)
      
1618. phenol/chloroform of purified PCR product after RE digestion - what are we trying to purify? (reply: 4)
      
1619. SYBR Green PCR Master Mix storage - (reply: 3)
      
1620. PCR and topo problem - (reply: 3)
      
1621. RT-PCR primer design at 3'-UTR or ORF? - (reply: 2)
      
1622. Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers?&# - (reply: 6)
      
1623. is PCR credible enough for validating my clones? - (reply: 5)
      
1624. How to calculate absolute fold change in expression by realtime PCR - Realtime PCR (reply: 2)
      
1625. unknown PCR bands - (reply: 4)
      
1626. Amplification of 3KB protein from Hela mRNA PCR PROBLEM - PCR Amplification of 3KB protein (reply: 6)
      
1627. Problem amplifying DNA from tissue - (reply: 4)
      
1628. qPCR for HKG and traditional PCR for GOI - (reply: 1)
      
1629. PCR or digestions for clone screen? - (reply: 5)
      
1630. Smeary bands in real-time RT-PCR - (reply: 7)
      
1631. high GC content in PCR? - (reply: 2)
      
1632. RT PCR - (reply: 1)
      
1633. Amplification of specific genes from cDNA - (reply: 3)
      
1634. How to purify PCR product? - (reply: 3)
      
1635. Touch down real-time PCR? - (reply: 1)
      
1636. PCR internal control - how does it work? (reply: 1)
      
1637. Primer design for environmental samples - Align sequences first? (reply: 3)
      
1638. False positive for PCR screening of insert - (reply: 1)
      
1639. not able to ligate PCR product into a vector... - what do you suggest? (reply: 2)
      
1640. why using Vent-exo polymerase? - (reply: 2)
      
1641. southern hybridizaion with 5' end labelled primer - (reply: 1)
      
1642. Programs for designing Primers - (reply: 6)
      
1643. DNA template for PCR reaction - (reply: 6)
      
1644. Disadvantages of using oligo-dT primers - (reply: 1)
      
1645. PCR chemistry - (reply: 4)
      
1646. Selective amplification problem in AFLP - (reply: 1)
      
1647. shorter PCR products after bisulfite treatment and cloning - (reply: 5)
      
1648. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
      
1649. Stool PCR and RFLPs - Campylobacter PCR from Stool (reply: 6)
      
1650. Do I need internal controls in MS-PCR? - Methylation specific PCR Internal controls (reply: 3)
      
1651. Blunt PCR cloning not working - (reply: 2)
      
1652. PCR Trouble shooting - PCR Trouble shooting (reply: 2)
      
1653. Genomic DNA Contamination of Real Time PCR - (reply: 4)
      
1654. ES cell genomic DNA as PCR template for screening - (reply: 5)
      
1655. How to eliminate primer dimer in MSP - (reply: 20)
      
1656. Problem with PCR amplification - (reply: 2)
      
1657. Band diffusion of my PCR product in electrophoresis - (reply: 3)
      
1658. Simple PCR - (reply: 1)
      
1659. do primers and dNTPs alter digestion efficiency? - (reply: 4)
      
1660. targetted pcr - targetted pcr (reply: 3)
      
1661. PCR from human genomic DNA - (reply: 4)
      
1662. Sequencing primer dilution - (reply: 2)
      
1663. magic? - got more product after PCR purification... (reply: 3)
      
1664. PCR for cloning - PCR (reply: 2)
      
1665. Optimization of PCR annealing temperature - (reply: 7)
      
1666. PCR with degenerate primer - (reply: 6)
      
1667. Strange PCR bands behaviour - (reply: 2)
      
1668. 1.3kb PCR only shows primer dimer - (reply: 2)
      
1669. Problem with PCR on DNA from paraffin section - (reply: 2)
      
1670. Titration of Mg2+ in RT-PCR - (reply: 3)
      
1671. RFLP PCR amplification - No amplification in PCR (reply: 2)
      
1672. RT-PCR primers for E. coli LacZ gene - (reply: 3)
      
1673. water instead of TE buffer to dissolved primers - (reply: 6)
      
1674. SOS: real time PCR standard curve - (reply: 1)
      
1675. Addition of pcr reagents to master mix - What order is best? (reply: 1)
      
1676. Serratia PCR - (reply: 6)
      
1677. primer reconstitution - (reply: 2)
      
1678. QuickChange mutagenesis: how to do it without kit? - What dNTP concentration should I use? (reply: 1)
      
1679. Question about PCR primer with degenerate base at 3' end - (reply: 2)
      
1680. Long distance (16 kb) PCR problem - (reply: 12)
      
1681. methylation Primers - Methyalted PCR (reply: 1)
      
1682. Primer for 4 genes sharing short consensus sequence - (reply: 2)
      
1683. what is the best pair of sequencing primer for puc 18 - (reply: 1)
      
1684. Looking for online primer design for SNaPshot - (reply: 1)
      
1685. pCI sequencing primer - (reply: 1)
      
1686. ask for an explanation to pcr ladder at high cycle number - help (reply: 2)
      
1687. about designing PCR primers - help (reply: 3)
      
1688. PCR primers: hairpin and dimers? - what can I do to avoid this? (reply: 4)
      
1689. 1st time real-time PCR questions - (reply: 1)
      
1690. BSP - favors amplification of unmethylated template? - (reply: 2)
      
1691. real-time pcr optimization problem - (reply: 4)
      
1692. PCR on PCR of cDNA smear - (reply: 1)
      
1693. RT-PCR amplification of an intron - has anyone tried this? (reply: 4)
      
1694. Reconstitute primers - (reply: 4)
      
1695. Which polymerase for bisulfite sequencing PCR - (reply: 3)
      
1696. PCR product - (reply: 5)
      
1697. Problems in reproducing the PCR results! - conventional PCR (reply: 3)
      
1698. Delta-delta Ct method and PCR efficiencies - (reply: 9)
      
1699. Multiplex PCR Problem - SOS (reply: 3)
      
1700. multiplex PCR for three sets of primers - (reply: 1)
      
1701. attach adaptors or PCR the restriction enzyme sites? - which one would you suggest and why (reply: 2)
      
1702. repeat PCR amplification problem - (reply: 2)
      
1703. PCR product purification - (reply: 3)
      
1704. to purify these PCR products or not? - preparation for restriction digest (reply: 5)
      
1705. inner primer for BSP PCR? - (reply: 6)
      
1706. How many mismatches in point mutation primers? - (reply: 3)
      
1707. Direct DNA sequencing - sequencing without PCR product (reply: 6)
      
1708. Software available for Multiplex PCR primer designing - (reply: 3)
      
1709. Picking stable clones by RT-PCR.. - (reply: 2)
      
1710. PCR of ligation products - (reply: 8)
      
1711. single primer PCR - use a single primer to amplify regions between inverted repeats (reply: 1)
      
1712. multiplex pcr trouble - some bands won't amplify (reply: 16)
      
1713. PRIMER design - primer dimers (reply: 1)
      
1714. Subcloing PCR fragments - (reply: 1)
      
1715. Why I keep getting no colonies from transformation of PCR products - help! (reply: 5)
      
1716. 3' - 5' activity of Taq DNA Polymerase - (reply: 2)
      
1717. ACE PCR - problems with PCR for angiotensin-converting enzyme (reply: 2)
      
1718. Amplify 4.5 kb pcr product without using special polymerase - (reply: 10)
      
1719. PCR master mix - (reply: 6)
      
1720. SOS! asymmetric PCR, no PCR product - (reply: 2)
      
1721. degenerate PCR vs low stringency PCR - (reply: 1)
      
1722. RT-PCR- help - Polyacrylmide Gel electrophrosis for PCR products (reply: 3)
      
1723. No DNA on gel, but get PCR product - (reply: 2)
      
1724. Mouse MSP primers - (reply: 11)
      
1725. PCR primer: 2 restriction sites? - (reply: 4)
      
1726. Primer Dimer problems in real-time PCR - (reply: 14)
      
1727. No PCR Product - Fungal Genomic PCR (reply: 9)
      
1728. primer orientation - (reply: 1)
      
1729. need help,why colony pcr of my blue has product what i need - (reply: 2)
      
1730. Degenerate primers for nested PCR? - (reply: 1)
      
1731. so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
      
1732. Looking for real time PCR workshops - real time PCR workshops (reply: 2)
      
1733. Amplification of the uncharacterized regions by Inverse PCR - what's the application since the target gene is inverted after it? (reply: 2)
      
1734. Reliable ChIP primers for Sp1? - (reply: 2)
      
1735. house-keeping primers for Cos7 cells - (reply: 4)
      
1736. increased primer dimer with template - (reply: 2)
      
1737. designing the sequencing primer - (reply: 2)
      
1738. How long should the DNA fragment be amplified by the two-step PCR? - (reply: 8)
      
1739. free software to examine if two primers will make dimers - (reply: 3)
      
1740. primer efficiency in real time PCR - need for a method explanasion (reply: 1)
      
1741. Mutagenic PCR unsuccessful :( - pls help.... (reply: 7)
      
1742. PCR High GC content - PCR High GC content (reply: 11)
      
1743. Testing primers - (reply: 3)
      
1744. PCR, contamination or not - (reply: 1)
      
1745. Me again...on type of polymerase and REs I should use... - (reply: 6)
      
1746. design of PRC primers of full ORF - (reply: 3)
      
1747. RT-PCR with an old DNaseI? - (reply: 3)
      
1748. Two round MSP with the same primer - (reply: 1)
      
1749. OD260 and free dNTP - (reply: 3)
      
1750. faint pcr bands to some, intense to some - (reply: 5)
      
1751. primer dimers - (reply: 4)
      
1752. Designing a Primer within an exon - Consesus required - (reply: 2)
      
1753. Nested primer PCR - (reply: 5)
      
1754. Contamination/no amplification in MSAP-PCR - (reply: 3)
      
1755. recommended pcr kits? - (reply: 8)
      
1756. No bands at all in gel for PCR products - (reply: 5)
      
1757. positive control for RT PCR - (reply: 2)
      
1758. Finding PCR Limiting Reagent - (reply: 5)
      
1759. Require help with Semi- quantitative PCR protocol - (reply: 10)
      
1760. problem wiht long pcr fragment - (reply: 2)
      
1761. Old telomerase - PCR elisa kit - can it work? - (reply: 2)
      
1762. difference between primer extension and RACE - (reply: 6)
      
1763. Amplifying products of different lengths - (reply: 2)
      
1764. Is RealTime PCR data enough for Telomerase activity? - (reply: 4)
      
1765. Taq PCR and A-tailing - (reply: 2)
      
1766. No insert for cloning PCR product into expression vector - (reply: 5)
      
1767. using gelatin for PCR - gelatin for PCR (reply: 3)
      
1768. designing QRT-PCR Primers - (reply: 11)
      
1769. Primer - (reply: 1)
      
1770. multiplex PCR - (reply: 4)
      
1771. Best way to purify PCR product for sequencing - (reply: 2)
      
1772. How can I get the 4.5kb Human typeI procollagen alpha1 chain cDNA Via RT-PCR fr - Seeking Help (reply: 14)
      
1773. OCT medium and real time pcr - (reply: 2)
      
1774. PCR Smear - (reply: 2)
      
1775. amplification fails to reach log linear phase - (reply: 1)
      
1776. More RT PCR questions: - can I amplify "everything?" (reply: 2)
      
1777. PCR off midiprep - problem with downstream reactions? (reply: 5)
      
1778. Ethidium Bromide vs Sybr Green stain (normal PCR) - (reply: 5)
      
1779. How many amplifications can you get with PCR? - (reply: 4)
      
1780. PCR master Mix problem...Plz help me out - PCR (reply: 8)
      
1781. Im stuck with my PCR for nearly 2 months! - i've no bands!! (reply: 5)
      
1782. RT-PCR on plasmid - (reply: 1)
      
1783. Digesting PCR products and cleaning up digestions - a few quick questions (reply: 4)
      
1784. Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
      
1785. Hybridation vs Normal PCR - (reply: 2)
      
1786. Detection of Bt gene in Corn Through PCR - High School Science Fair - (reply: 6)
      
1787. peculiar PCR inhibition - (reply: 11)
      
1788. separation of pcr products of similar sizes in agarose gel - (reply: 1)
      
1789. Total cDNA PCR - (reply: 3)
      
1790. Can I add additional sodium ion to PCR reaction buffers? - (reply: 5)
      
1791. Troubleshooting of basic PCR - (reply: 12)
      
1792. PCR product precipitation - (reply: 4)
      
1793. High Fidelity DNA Polymerase: how many mutations per every kb of amplified DNA o - (reply: 10)
      
1794. Cloning PCR product - double bands or heterozygote (reply: 5)
      
1795. no band in PCR ! - (reply: 4)
      
1796. virtual PCR - (reply: 3)
      
1797. Trouble with standard curves for real-time PCR - (reply: 6)
      
1798. Real-time PCR machines for DNA quantitation - (reply: 5)
      
1799. Contamination from media (MRS) in PCR - (reply: 1)
      
1800. purification by gel? - after a first round of PCR (reply: 2)
      
1801. rat 18s rRNA primer - designing primers (reply: 1)
      
1802. Does Reverse Transcription have to be done using PCR - (reply: 6)
      
1803. Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
      
1804. how to improve the efficiency of my FQ-PCR - I can not get a beautiful amplification curve (reply: 3)
      
1805. Which technique is Better: Southern Blotting or PCR?? - (reply: 6)
      
1806. PCR of AmpR gene - PCR of Amp gene (reply: 1)
      
1807. Condition for amplifying >6k PCR - Long PCR amplification (reply: 12)
      
1808. PCR reactions - What condition should I use? (reply: 5)
      
1809. False positive for colony PCR - (reply: 3)
      
1810. inverse PCR or promoter trapping - (reply: 2)
      
1811. Overlap PCR - (reply: 2)
      
1812. Taq polymerase - (reply: 5)
      
1813. Designing primers for multiplex - BLASTing lots of primers (reply: 3)
      
1814. Methylation & PCR - (reply: 1)
      
1815. optimization of pcr conditions - (reply: 9)
      
1816. TaqMan probes, pcr mix - (reply: 1)
      
1817. Why random primers for RT-qPCR - (reply: 1)
      
1818. What way would you suggest to clean PCR products? - (reply: 4)
      
1819. Real Time PCR for Telomerase - (reply: 4)
      
1820. Primer design question - Use of primer 3 (reply: 3)
      
1821. Can I make four substitutions (mutagenesis) using PCR at a time? - help me out, pls :) (reply: 7)
      
1822. Long PCR for amplification from plasmid - (reply: 5)
      
1823. RT-PCR detection limits - (reply: 1)
      
1824. PCR after Sonication... - (reply: 10)
      
1825. PCR Optimization for 50-100 base transcript - How do I increase yield of small PCR product? (reply: 4)
      
1826. PCR trouble - (reply: 16)
      
1827. Primer extension - yeast tRNA as a negative control (reply: 3)
      
1828. cloning of PCR product - (reply: 1)
      
1829. Dimers and weird bands after Bis-PCR - (reply: 5)
      
1830. KOD plus for PCR - (reply: 1)
      
1831. Taq polymerase that can run 100 cycles? - (reply: 12)
      
1832. RT-PCR Primer design - which region? - (reply: 2)
      
1833. primer design for MSP - How many CpG in the primer? (reply: 2)
      
1834. Lower size band in genomic PCR with 300 bp missing - (reply: 12)
      
1835. PCR - RFLP of plant cpDNA - Can I use several restriction enzyms together? (reply: 2)
      
1836. PCR amplicon size and contamination - (reply: 4)
      
1837. DNA PCR on blood sample - help-Genomic DNA PCR from clogged blood. (reply: 3)
      
1838. Gelatin for PCR - (reply: 2)
      
1839. question of getting a 75bp PCR product - (reply: 1)
      
1840. Question about gel purification of PCR product - (reply: 1)
      
1841. DNA amplification - troubleshooting - (reply: 4)
      
1842. nested PCR - (reply: 3)
      
1843. Cloning using RT-PCR for long fragment of 6kb - (reply: 1)
      
1844. No PCR products in MSP with MethPrimer designed primers - (reply: 2)
      
1845. No PCR product - troubleshooting PCR (reply: 12)
      
1846. Taq polymerase dilution? - (reply: 3)
      
1847. How to use Bst polymerase for PCR? - (reply: 8)
      
1848. help pcr cloning problem - help pcr cloning problem!!!!!1 (reply: 2)
      
1849. PCR stop working - (reply: 2)
      
1850. problems with mix of MSP's primers - (reply: 2)
      
1851. pcr ligation - (reply: 2)
      
1852. [PCR products] - (reply: 1)
      
1853. genomic DNA PCR - RE digestion of gDNA before amplification (reply: 3)
      
1854. real time PCR with sybr green - having problems validating my knock down (reply: 3)
      
1855. shorter unspecific PCR product - (reply: 7)
      
1856. PCR an insert in a vector with wrong product size - (reply: 1)
      
1857. Visualization of pcr product before sequencing - (reply: 5)
      
1858. RNA extraction with TRizol and RT-PCR - (reply: 8)
      
1859. Real-time PCR probe degradation - when do they degrades? (reply: 4)
      
1860. design primer for PCR.. - (reply: 3)
      
1861. question about PCR clone a cDNA - (reply: 9)
      
1862. Amplitaq Gold pcr master mix - Protocol question (reply: 1)
      
1863. From MSP primer to gene - how to recalculate original DNA sequence? (reply: 4)
      
1864. realtime pcr validating the results of microarray? - (reply: 7)
      
1865. positive NTC in real time PCR - (reply: 8)
      
1866. gel extraction of pcr products - (reply: 3)
      
1867. Design primer - (reply: 2)
      
1868. PCR for more than 5 kb - (reply: 4)
      
1869. cDNA cloning problem - No product in PCR (reply: 5)
      
1870. MSP primers not working - (reply: 1)
      
1871. Is polymerase slippage related to low fidelity? - DNA polymerase slippage during PCR (reply: 2)
      
1872. Quick cloning question - Purification of PCR fragment (reply: 1)
      
1873. No PCR product after BSP, but dimers - (reply: 5)
      
1874. problems with bisulfite PCR - (reply: 8)
      
1875. real time pcr problem - (reply: 4)
      
1876. extra sequence in primers - (reply: 4)
      
1877. Inactivation of Thermo Sequenase / Taq polymerase - (reply: 1)
      
1878. Amplification problems - (reply: 9)
      
1879. PCR TEST FOR DIAGONISTIC - (reply: 3)
      
1880. Sybr RT PCR problem - (reply: 4)
      
1881. Primer Programm for Sequencing - (reply: 3)
      
1882. calculating Tm of primers - (reply: 8)
      
1883. real time PCR trouble - real time PCR trouble (reply: 9)
      
1884. AmpliTaq Gold instead of TaqMan Universal PCR Mix in Real-time? - (reply: 3)
      
1885. Primer extension problem - there are too many bands!! (reply: 1)
      
1886. RT-PCR for large fragment - (reply: 7)
      
1887. real time RT-PCR in bacteria - quantitative PCR (reply: 2)
      
1888. nested PCR after bisulphite - (reply: 3)
      
1889. How to design random primers - (reply: 2)
      
1890. design pcr primers - (reply: 3)
      
1891. PCR amplification of >6Kbp DNA - (reply: 2)
      
1892. RT-PCR problem - negative control (reply: 1)
      
1893. How to store PCR product - (reply: 3)
      
1894. Random genoic amplification for microarray - (reply: 4)
      
1895. PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
      
1896. Real-time PCR - contamination or not? - (reply: 6)
      
1897. Does it help to add antisense primer first for bisulfite PCR? - (reply: 2)
      
1898. Degenerate PCR troubleshooting - (reply: 3)
      
1899. Amount of DNA template in PCR reaction - (reply: 2)
      
1900. A problem of quantitative PCR - (reply: 2)
      
1901. gDNA PCR - (reply: 2)
      
1902. maximum size for PCR - (reply: 3)
      
1903. primer design problem - (reply: 1)
      
1904. Bisulfite treated DNA specific primer set critique? - (reply: 18)
      
1905. changing dNTP -> changing PCR conditions? - (reply: 3)
      
1906. 60 bp PCR and primer dimers - (reply: 6)
      
1907. Can I directly digest PCR products? - (reply: 9)
      
1908. faint bands of PCR products - (reply: 5)
      
1909. Real Time PCR optimization - standard curve generation (reply: 3)
      
1910. PCR contamination with human DNA - (reply: 5)
      
1911. No product/primer dimers after BSP - (reply: 1)
      
1912. bisulfite modification after pcr - (reply: 2)
      
1913. Primer Express Software - Probe Design (reply: 8)
      
1914. Degenerate PCR troubleshooting - (reply: 9)
      
1915. Primer design:Is it ok to omit extra bases after RE site? - (reply: 4)
      
1916. effect of ethanol and dna concentration in pcr - (reply: 3)
      
1917. Problem in Multiplex PCR - Multiplex PCR (reply: 3)
      
1918. PCR small framents - (reply: 2)
      
1919. Problems with methylation analysis!!! - Primers crossreaction etc. (reply: 3)
      
1920. real time PCR (inconsistant results) - (reply: 2)
      
1921. Double digestion of PCR product and ligation - (reply: 5)
      
1922. PCR optimization - (reply: 12)
      
1923. The same PCR gives different results?? - PCR (reply: 1)
      
1924. no band obtain after PCR - (reply: 2)
      
1925. Inconsistent RT-PCR results - (reply: 1)
      
1926. Problem with PCR - (reply: 4)
      
1927. Odd curve of Gel purified PCR product - (reply: 5)
      
1928. primers for SoE - (reply: 1)
      
1929. inconsistent PCR - (reply: 7)
      
1930. Trouble with Inverse PCR - (reply: 8)
      
1931. RAPD PCR help - (reply: 2)
      
1932. multiple band on PCR - (reply: 5)
      
1933. What is the role for gelatin in PCR buffer - (reply: 1)
      
1934. PCR enhancer - (reply: 5)
      
1935. PCR amplifying 50 bp DNA fragment - (reply: 2)
      
1936. Discussion: ChIP PCR control - (reply: 12)
      
1937. Help: Question on PCR Cleaning - PCR Cleaning Problem (reply: 3)
      
1938. designing flanking primers around msp primers - (reply: 1)
      
1939. Primer for BSP - (reply: 1)
      
1940. Help: (PCR) Increase template, but NO Increase in product - (reply: 5)
      
1941. BSP cycles and primers - Is it OK to use the same primer set but with more (reply: 6)
      
1942. PCR purification basics? - (reply: 6)
      
1943. primer hairpins and self complementation - online primer checker.. (reply: 2)
      
1944. No PCR products with the ChIP DNA - (reply: 5)
      
1945. Problem amplifying 3 kp fragment from a plasmid - (reply: 4)
      
1946. primer does not bind - help (reply: 2)
      
1947. PCR problems - problems (reply: 2)
      
1948. reconstitute primer - (reply: 4)
      
1949. PCR mix storage - (reply: 1)
      
1950. RT-PCR scale-down makes more sensitive? - (reply: 3)
      
1951. Troubleshooting problem in PCR for bisulfite Treated DNA - (reply: 1)
      
1952. Purification of short PCR product - (reply: 4)
      
1953. No PCR product after bisulfite treatment :( - (reply: 3)
      
1954. PCR Supermix vs Roche - (reply: 3)
      
1955. cloning problem - sticky end cloning with PCR insert - (reply: 3)
      
1956. SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
      
1957. Online primer design - (reply: 7)
      
1958. PCR contamination by recombination - (reply: 1)
      
1959. DNase1 problem in PCR - (reply: 6)
      
1960. RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
      
1961. Real-time PCR questions (Mx3000P) - Need some help with interpretation (reply: 7)
      
1962. T4 DNA poymerase - T4 DNA polymerase (reply: 5)
      
1963. TOPO XL cloning with 7.5kb PCR product - troubleshooting help (reply: 1)
      
1964. Unmethylated primer control - (reply: 4)
      
1965. PCR MUTAGENESIS PROBLEM - SiteDirected Mutagenesis - Problem (reply: 3)
      
1966. Cloning large pcr product - efficiency (reply: 2)
      
1967. help me to choose a high fidelity taq polymerase - (reply: 6)
      
1968. how much total RNA do you use for RT-PCR - (reply: 6)
      
1969. One Step RT-PCR cloning problem - (reply: 2)
      
1970. Extremely PCR scale down - (reply: 4)
      
1971. Manipulation with PCR components - Laminar, PCR box? (reply: 3)
      
1972. mastermix for Real-time PCR - (reply: 2)
      
1973. Real time PCR - small peaks in the melt curve - (reply: 5)
      
1974. PCR from genomic DNA - (reply: 1)
      
1975. storage of taq polymerase - (reply: 4)
      
1976. PCR on Leishmania DNA - (reply: 1)
      
1977. is it effective to use a primer of SD rat to Wistar rat in PCR - help (reply: 2)
      
1978. Buffers for storing and diluting of dNTP - (reply: 3)
      
1979. What kind of statistic methods? - RT-PCR (reply: 2)
      
1980. Help a first time cloner with PCR! - First time cloning (reply: 5)
      
1981. Custom Primers - (reply: 2)
      
1982. Primers for RT reaction - (reply: 6)
      
1983. PCR interference? - (reply: 2)
      
1984. Real Time PCR - Relative Quantitation - Choice of calibrator and calculations (reply: 1)
      
1985. Primer Tm - How low can you go? - (reply: 3)
      
1986. PCR product size with a Roche Lightcycler? - (reply: 1)
      
1987. How to prepare primer solution - (reply: 7)
      
1988. Primers design: how to check for mispriming? - (reply: 2)
      
1989. Internal Control RNA & RT-PCR - (reply: 1)
      
1990. PCR from genomic DNA - (reply: 6)
      
1991. Methylated-Specific PCR - (reply: 6)
      
1992. standard curves - real time pcr (reply: 3)
      
1993. primer storage - (reply: 7)
      
1994. EDTA - real time PCR (reply: 3)
      
1995. Cloning and PCR Problems - (reply: 1)
      
1996. Pls check my bisulphite sequencing primers - (reply: 12)
      
1997. rt-PCR false postitives - (reply: 2)
      
1998. Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
      
1999. Verifying the microarray - real time PCR (reply: 1)
      
2000. How to setup control for bisulfite sequencing PCR - (reply: 2)
      
2001. RT-PCR some questions - (reply: 2)
      
2002. Bad Primer - (reply: 2)
      
2003. Cloning of sequence with PCR added sites - BstBI and BclI (reply: 2)
      
2004. PCR problems - (reply: 3)
      
2005. long range PCR problem - unable to amplify 12 kb fragment (reply: 4)
      
2006. delta delta Ct method - Real time PCR (reply: 4)
      
2007. first strand or double strand cDNA before PCR: RT-qPCR - (reply: 3)
      
2008. Reverse-transcriptase PCR troubleshoot HELP - (reply: 13)
      
2009. reducing primer dimer - (reply: 1)
      
2010. PCR troubleshooting - Primers (reply: 3)
      
2011. PCR problem amplifying 4 kb DNA - (reply: 5)
      
2012. Calculating correct melting temperature for primers - (reply: 2)
      
2013. primer melting temperature - (reply: 2)
      
2014. How can I add sequences to DNA using PCR? - (reply: 1)
      
2015. Bisulfite Sequencing Primer Design - (reply: 3)
      
2016. end of primer - (reply: 2)
      
2017. degenerate primer desgining - degenerate primer desgining (reply: 5)
      
2018. T/A cloning of cDNA? - PGEM-T vs pCR 2.1 T/A TOPO? (reply: 3)
      
2019. Reamplification of RT-PCR product - (reply: 2)
      
2020. PCR primer dimer? - (reply: 7)
      
2021. checking primer - checking primer (reply: 5)
      
2022. How many times for a run -real time pcr - (reply: 1)
      
2023. PCR primer Tm and concentration question - (reply: 3)
      
2024. How to avoid secondary structure in primer - (reply: 2)
      
2025. PCR reamplifying problem - (reply: 5)
      
2026. subcloning of PCR product, please help! - subcloning problems (reply: 12)
      
2027. cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
      
2028. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
      
2029. primer design - checking off-target site - (reply: 5)
      
2030. real-time PCR - (reply: 5)
      
2031. NAC (No Amplification Control) - (reply: 2)
      
2032. Realtime PCR standard curves - (reply: 8)
      
2033. What is the distance between a forward and reverse primer - (reply: 1)
      
2034. Real time PCR supermix - (reply: 4)
      
2035. differents results in PCR and sequencing!! - (reply: 3)
      
2036. PCR contamination mystery - (reply: 2)
      
2037. Does residual ethanol in DNA inhibit PCR? - ? (reply: 1)
      
2038. Real-time PCR amplification curve up and down - (reply: 3)
      
2039. Resuspend probe in real time pcr - (reply: 1)
      
2040. Which RT primer is better for qPCR - (reply: 2)
      
2041. background signal-real time pcr - (reply: 1)
      
2042. HEPA filter - and PCR contamination (reply: 1)
      
2043. quencher choice in real time pcr - (reply: 2)
      
2044. Problem with amplifying DNA from FTA cards - Is there a special trick involved? (reply: 1)
      
2045. PCR; Perhaps someone can explain? - PCR; Primer (reply: 3)
      
2046. Why different amounts of forward and reverese primers in RT-QPCR - (reply: 2)
      
2047. Degenerate primers - (reply: 1)
      
2048. primer optimization - (reply: 1)
      
2049. Mx 3000 machine-real time pcr - (reply: 1)
      
2050. PCR on plasmids? - (reply: 2)
      
2051. PCR with T7 promoter/T7 terminator - (reply: 5)
      
2052. Pfx PCR problems - (reply: 1)
      
2053. primers for Q-PCR - (reply: 2)
      
2054. Storage of RT-PCR products - (reply: 2)
      
2055. Detecting 12 nt differences in a 600+ bp pcr product - (reply: 2)
      
2056. PCR of a large DNA fragment - (reply: 3)
      
2057. multiplex PCR - template and primer concentration (reply: 2)
      
2058. Pipetting on ice - during PCR (reply: 5)
      
2059. PCR contamination? - (reply: 3)
      
2060. Sometimes i get bands, sometimes i get nothing for my PCR - Strange PCR Results (reply: 1)
      
2061. Primers and templates - Primer and template concentrations (reply: 2)
      
2062. Multiplex PCR troubleshooting - (reply: 6)
      
2063. PCR inhibition - (reply: 1)
      
2064. Real Time qPCR - qPCR SYBR greenI primer design (reply: 5)
      
2065. PCR product on gel as smear - DNA isolated from paraffin blocks why smear? (reply: 1)
      
2066. Primers for species identification - Know any good? (reply: 6)
      
2067. melt curves for SYBR Green real-time PCR driving me nuts!! - melt curves real-time PCR (reply: 6)
      
2068. Genotype ratio with quantitative PCR - (reply: 1)
      
2069. The perfect number of CpGs in MSP primer - (reply: 8)
      
2070. can't get rid of DNA contamination even with DNase for RT-PCR - (reply: 3)
      
2071. Primer Express 2.0 - crashes (reply: 1)
      
2072. PCR master mix or super mix - PCR technique (reply: 1)
      
2073. relative DNA quantification from PCR - calculation of band intensity from PCR (reply: 2)
      
2074. PCR screeing for positive transormation - (reply: 1)
      
2075. real-time RT-PCR one cell - (reply: 2)
      
2076. Contamination in PCR cloning - (reply: 3)
      
2077. Primers - (reply: 1)
      
2078. PCR fragment analysis - please help! - PCR fragment analysis - please help! (reply: 2)
      
2079. Doublet after real time PCR! - (reply: 3)
      
2080. Hot start PCR - (reply: 6)
      
2081. dNTP calculation - (reply: 5)
      
2082. mutilple bands after RT-PCR - (reply: 2)
      
2083. Random primers-real time PCR - (reply: 1)
      
2084. How to measure the concentration of primers? - (reply: 2)
      
2085. detection of transgene by PCR - (reply: 1)
      
2086. Probes or primers that span introns - (reply: 6)
      
2087. Tm for my PCR product (not Tm for the primers!) - (reply: 3)
      
2088. Problem with second PCR - 1st PCR product and 2nd PCR product are different (reply: 1)
      
2089. Primer3 - (reply: 1)
      
2090. Cloning a large PCR product - trouble cloning large DNA (reply: 3)
      
2091. double sized PCR product from cloned cDNA - (reply: 6)
      
2092. real-time pcr plasmid standards - (reply: 4)
      
2093. RT-PCR and RE cut site-will this work? - (reply: 3)
      
2094. How much of the starting amounts for Real time PCR? - (reply: 1)
      
2095. PCR of plasmid miniprep from Agrobacterium - Can't get PCR product out of plasmid miniprep (reply: 1)
      
2096. PCR parameters - (reply: 2)
      
2097. what primers specific for B.stearothermophilus?Help pls. - (reply: 5)
      
2098. Unequal amplification efficiency of heat blocks - (reply: 4)
      
2099. Can Taq polymerase be left at room temperature for a long time - (reply: 4)
      
2100. PCR colony screening - (reply: 9)
      
2101. PCR off of Genomic DNA problems! - -why is my gene without introns showing up?- (reply: 3)
      
2102. help regarding primers reconstitution - (reply: 4)
      
2103. problem with my PCR - primer dimer. - (reply: 1)
      
2104. PCR - Long PCR with short primers (reply: 1)
      
2105. invitrogen Superscript one step RT PCR kit - Efficiency of kit (reply: 4)
      
2106. Calculating Tm of a PCR product? - (reply: 6)
      
2107. PCR techniques (help me) - (reply: 5)
      
2108. Sequencing of PCR products - (reply: 5)
      
2109. quantitation of PCR bands - (reply: 1)
      
2110. a Takara TA taq DNA polymerase protocol - (reply: 1)
      
2111. Can BSA help PCR? - (reply: 3)
      
2112. how long a primer should I use? - (reply: 1)
      
2113. TA cloning with ABgene's extensor HiFi polymerase - (reply: 1)
      
2114. problem with PCR --using fragment as template - (reply: 12)
      
2115. PCR scaling up doesn't work - (reply: 7)
      
2116. real time PCR kit components - (reply: 2)
      
2117. Screening of TA cloning by T7/Sp6 PCR possible? - (reply: 2)
      
2118. identifying exon and intron for primer design - (reply: 2)
      
2119. Can I PCR amplify a ligation product directly - (reply: 3)
      
2120. PCR Genomic DNA - PCR Genomic DNA (reply: 1)
      
2121. difficulty about cloning 3.5kb PCR product - (reply: 1)
      
2122. PCR question - (reply: 3)
      
2123. what is the "tailed primer and taq primer"? - (reply: 2)
      
2124. Real Time PCR and melting curve - (reply: 2)
      
2125. PCR to the PCR!! - (reply: 6)
      
2126. threshold for real time PCR - (reply: 1)
      
2127. contamination in real-time PCR - (reply: 32)
      
2128. TA cloning with short PCR products - use of TA cloning kit (reply: 4)
      
2129. Ligation of PCR products - (reply: 1)
      
2130. How do you prepare PCR stock solution to minimize pipetting - (reply: 37)
      
2131. How do you mix PCR reactions - swish, flick or nothing? - (reply: 44)
      
2132. multiplex PCR for 9 exons - (reply: 5)
      
2133. Promoter Cloning - PCR amplification of a promoter and cloning (reply: 1)
      
2134. Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system? (reply: 1)
      
2135. PCR an insert in a vector with wrong product size - I can not amplify my fragment with a normal PCR (reply: 8)
      
2136. tRNA and Real time RT-PCR - (reply: 1)
      
2137. Problem with TA PCR cloning - no insert in white colonies - (reply: 12)
      
2138. Designing methylation primers on long CpG island - (reply: 14)
      
2139. Difficutly go separate product from primer dimers - Methylation specific PCR (reply: 1)
      
2140. 3 bands on RT-PCR - (reply: 4)
      
2141. PCR product wrong size (too high) - PCR (reply: 2)
      
2142. DNaseI have affect with RT-PCR or not - (reply: 3)
      
2143. PCR help - (reply: 5)
      
2144. RT- PCR? - (reply: 2)
      
2145. ABI7000 + SYBR GREEN I - quantitative PCR (reply: 4)
      
2146. real time PCR primer for methylation - (reply: 2)
      
2147. designing gene-specific primer for cDNA synthesis - (reply: 5)
      
2148. Determine the RNA concentraiton for linear PCR - (reply: 1)
      
2149. Purification of small (100bp) PCR products - (reply: 7)
      
2150. contamination in real-time pcr negative control - (reply: 12)
      
2151. Primer design for unknown gene - (reply: 4)
      
2152. TOPO TA pCR 2.1 cloning - (reply: 1)
      
2153. How to design primers for CpG, CpNpG, CpHpH methylation - Bisulfite Sequencing PCR (reply: 2)
      
2154. Real-time PCR problems - (reply: 3)
      
2155. Designing primers from amino acid sequence - cloning problem (reply: 3)
      
2156. software to find restriction site and TM for primer - (reply: 7)
      
2157. RT-PCR for an intronless gene - (reply: 6)
      
2158. Large scale PCR to get milligram of DNA - (reply: 6)
      
2159. PCR woes - intermittent empty lanes (reply: 6)
      
2160. real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
      
2161. Extrremely bright bands in the WELLs of PCR agarose gel - Bright and sharp bands in the PCR gel well (reply: 9)
      
2162. PCR problem: DISTINCT bands far shorter than target! - (reply: 3)
      
2163. Real-time PCR and high Ct value - (reply: 1)
      
2164. genotyping transgenic mice with PCR - (reply: 1)
      
2165. High fidelity RT-PCR enzymes - looking for choices... (reply: 5)
      
2166. Failed to amplify a 2.1 kb PCR product - (reply: 3)
      
2167. Reuse of PCR purification column - (reply: 1)
      
2168. About PfuTurbo® DNA Polymerase and Pfu DNA Polymerase - (reply: 1)
      
2169. Problem with DNase treatment of RNA for RT-PCR - (reply: 1)
      
2170. realtime RT-PCR/internal control - (reply: 3)
      
2171. PCR and electrophoresis - help finding missing bands! (reply: 3)
      
2172. PCR LABORATORY - to avoid contaminations... (reply: 1)
      
2173. sense/antisense specific pcr - design of sense/antisense specific pcr primers (reply: 1)
      
2174. PCR contamination - (reply: 1)
      
2175. Problem with genomic DNA amplification - (reply: 7)
      
2176. PCR low amplification - (reply: 2)
      
2177. Realtime RT-PCR problem - no product - (reply: 6)
      
2178. RT-PCR gone bad - (reply: 4)
      
2179. product size for real-time PCR primer - (reply: 3)
      
2180. Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
      
2181. RT-PCR - a lot of wrong size fragments. - (reply: 2)
      
2182. Which vector for cloning big PCR fragments? - (reply: 3)
      
2183. Efficiency Real-Time PCR - Real-time PCR (reply: 3)
      
2184. microsatellite PCR from poor quality/trace amounts of DNA - (reply: 3)
      
2185. how to produce a high-quality cDNA template for RT-PCR - produce a high-quality cDNA template (reply: 4)
      
2186. Asymmetric PCR for generation of single stranded DNA - (reply: 5)
      
2187. PCR Ligation cloning - (reply: 2)
      
2188. GC content of PCR primers - (reply: 3)
      
2189. real time PCR primers - (reply: 2)
      
2190. influence of MgCl2 in PCR - (reply: 7)
      
2191. Colony PCR for clone screen - Colony PCR before sending sequence (reply: 4)
      
2192. RT-PCR - (reply: 1)
      
2193. mC not in CpG? Primer selection - (reply: 1)
      
2194. cpgware primer design, does it work - (reply: 4)
      
2195. TOPO-TA Cloning PCR product - Is amplicilin selection engough? (reply: 4)
      
2196. RT-PCR - 2 kb fragment in RT-PCR (reply: 2)
      
2197. PCR does not work - what is the problem? (reply: 2)
      
2198. Who will give me a software to design primer for PCR? - (reply: 3)
      
2199. old degraded samples. DNA extraction & PCR - (reply: 3)
      
2200. No PCR Product for Sequencing - (reply: 5)
      
2201. PCR Primer Design with restriction sites - (reply: 1)
      
2202. PCR - No bands anymore in well functioning PCR - (reply: 6)
      
2203. RT-PCR - (reply: 4)
      
2204. problems with PCR long target gene - (reply: 2)
      
2205. Hotstart in PCR - (reply: 1)
      
2206. No PCR Product - (reply: 2)
      
2207. primers with different Tms - PCR amplification using primers with different Tms (reply: 9)
      
2208. PCR comparison of gene expression - (reply: 5)
      
2209. Reamplification of PCR products - (reply: 2)
      
2210. "PCR ghosts" - :wacko: (reply: 2)
      
2211. The weirdest PCR ever! - 8kb band in 1min. (reply: 3)
      
2212. Sequence input for bisulfite PCR primer design - (reply: 1)
      
2213. Primer design for MSP queries - (reply: 3)
      
2214. Gradient PCR - (reply: 2)
      
2215. Database software to catalogue / archive primers - Database software to catalogue / archive primers (reply: 1)
      
2216. PCR Failure..pls help! - (reply: 3)
      
2217. PCR with DMSO - (reply: 12)
      
2218. Real Time PCR Annealing vs. Melting - Relation of anneal temp rxn to melt temp primer (reply: 2)
      
2219. mass balance in PCR - (reply: 3)
      
2220. Is there a PCR length limitation after Bisulfite treatment? - (reply: 3)
      
2221. PCR blood old samples - (reply: 2)
      
2222. RT-PCR with B-cells as template - (reply: 1)
      
2223. RT-PCR of different cell lines - (reply: 1)
      
2224. PCR for ChIP - PCR for ChIP not working (reply: 3)
      
2225. Bisulfite sequencing PCR problem - no products (reply: 3)
      
2226. primer design for bisulphite treated DNA - (reply: 1)
      
2227. methylated genomic DNA PCR - (reply: 1)
      
2228. PCR more difficult in methylated CpG region? - (reply: 7)
      
2229. primer dimers - what exactly are primer dimers (reply: 2)
      
2230. PCR of unknown DNA - PCRing unknown DNA with known linkers (reply: 2)
      
2231. primer design: end-point vs. RT - (reply: 1)
      
2232. Quantification of PCR using delta-delta Ct method. - Quantification (reply: 1)
      
2233. Primers for unmethylated DNA work too well! - (reply: 2)
      
2234. ligation of pcr product - (reply: 4)
      
2235. software to check the primer dimer - (reply: 3)
      
2236. TAQ polymerase is needed - TAQ polymerase for 60 cycles (reply: 1)
      
2237. Control for primer extension - (reply: 2)
      
2238. How to determine PCR annealing temperature - (reply: 9)
      
2239. BSP and MSP primer check - (reply: 2)
      
2240. sometimes I get it sometimes I dont - My PCr does not work always (reply: 9)
      
2241. Using a big primer in PCR - (reply: 4)
      
2242. allele-specific PCR - detection of SNPs by allele-specific amplification (reply: 4)
      
2243. No PCR product - Environmental samples (reply: 2)
      
2244. How to remove PCR inhibitors from DNA - (reply: 7)
      
2245. How to get rid of PCR primer dimer - (reply: 65)
      
2246. Problem cloning PCR product into double-cut vector - (reply: 3)
      
2247. designed primer more powerful? - (reply: 1)
      
2248. RT-PCR primer design - exon-exon primer design (reply: 5)
      
2249. How to remove DNA for quantitative PCR - (reply: 5)
      
2250. Primer design - PCR for cloning (reply: 6)
      
2251. Long-distance PCR problem - help (reply: 3)
      
2252. Promoter amplification.... - (reply: 7)
      
2253. PCR Contamination issues - PCR false positives (reply: 106)
      
2254. 1-2kb PCR products TOPO cloning troubles - help (reply: 4)
      
2255. no template but still got exact size bands???!!! - PCR genotyping problem (reply: 1)
      
2256. RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (reply: 1)
      
2257. PCR specificity problem - (reply: 5)
      
2258. PCR product for ligation - no PCR product from PCR product (reply: 1)
      
2259. How to qualify PCR productiion concentration? - (reply: 1)
      
2260. availabre primers for MSP - (reply: 1)
      
2261. real time PCR (using Sybr green) - (reply: 1)
      
2262. Strange mobility behavior of PCR products on agarose gel - EB stained vs Gelstar stained (reply: 2)
      
2263. product dimers with GenomeWalker kit - how to deal with them? (reply: 2)
      
2264. PCR or RT-PCR? - (reply: 3)
      
2265. PCR failing - (reply: 18)
      
2266. 3' end of forward primer mismatch - (reply: 5)
      
2267. Bisulfite sequencing and PCR issues - (reply: 1)
      
2268. Problem with PCR on difficult template - How to amplify GC-rich region? (reply: 6)
      
2269. PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
      
2270. DNA quantification - How to quantify my PCR product before sequencing?? (reply: 8)
      
2271. Weak RT-PCR products? - (reply: 1)
      
2272. RACE - specific primers in first strand synthesis (reply: 4)
      
2273. RT PCR - dilution prior to PCR (reply: 2)
      
2274. cloning PCR fragment - PCR fragment is not getting cloned in the vector (reply: 2)
      
2275. qPCR Primer Design and Genomic DNA Amplification - (reply: 1)
      
2276. checking for inserts with colony PCR - (reply: 6)
      
2277. Poor PCR yield - (reply: 4)
      
2278. Primer Designer Online - Pipleline use of designer (reply: 1)
      
2279. Well adding Sybr green in a PCR mix - to detect quicker than by electrophoresis PCR prod (reply: 4)
      
2280. PCR-Check, Screen-Check, MiniPrep - HUH?? - PCR product transformed, grown on AMP, no mini (reply: 2)
      
2281. does anyone know real-time methyaltion pcr? - (reply: 1)
      
2282. Cloning a long PCR product - (reply: 2)
      
2283. Problem in PCR cloning - (reply: 7)
      
2284. If the primer is dimer,it's ok? - (reply: 5)
      
2285. methylation primers - problems with primers (reply: 1)
      
2286. Troubleshoot PCR smear problem - all I got was smear!! (reply: 71)
      
2287. degenerate PCR - (reply: 2)
      
2288. cloning size is not correct - 2 bands on the PCR product (reply: 2)
      
2289. PCR amplification - PCR amplification (reply: 6)
      
2290. Multiplex PCR primer designing - (reply: 4)
      
2291. Smear after second PCR - (reply: 2)
      
2292. still more about cloning PCR products - (reply: 9)
      
2293. single cell PCR - (reply: 8)
      
2294. Annealing temperature for PCR - (reply: 3)
      
2295. Invitrogen platinum pfx enzyme not so fantastic - problems with pcr product gel migration (reply: 1)
      
2296. Genomic DNA amplification - (reply: 4)
      
2297. PCR works first time, then not! - (reply: 1)
      
2298. RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
      
2299. total rna extraction from whole blood - for rt pcr (reply: 3)
      
2300. PCR is6110 Tuberculosis - Non specific amplification (reply: 2)
      
2301. PCR from BAC - (reply: 1)
      
2302. Bisulfite modification kits and PCR - (reply: 5)
      
2303. Trouble shooting RT-PCR - (reply: 4)
      
2304. Transformation and PCR questions - (reply: 2)
      
2305. Advice for buying PCR workstation - (reply: 1)
      
2306. PCR contamination in negative control - (reply: 3)
      
2307. RNA isolation from mouse blood - RNA for RT-PCR (reply: 1)
      
2308. PCR Cloning - primer design and CpG methylation (reply: 4)
      
2309. 96 Well PCR clean up - Looking for a reliable product (reply: 3)
      
2310. cloning from PCR - (reply: 25)
      
2311. PCR cloning - PCR cloning (reply: 2)
      
2312. sowing pcr? - (reply: 5)
      
2313. real-time PCR - How to design primers (reply: 2)
      
2314. How to identify plasmid - using enzyme and PCR or other? (reply: 2)
      
2315. Which gene is the best internal control for RT-PCR - (reply: 5)
      
2316. BiPolar Clamps on PCR primers for TGGE - (reply: 1)
      
2317. PCR primers - PCR primers for RT-PCR (reply: 1)
      
2318. multiplex primers - (reply: 6)
      
2319. Real Time PCR problem - different dilutions have the same Ct - (reply: 6)
      
2320. Bisulfite PCR: Smear - Can't amplify a certain sequence (reply: 4)
      
2321. RT-PCR;Northern-blotting - internal standard (reply: 4)
      
2322. PCR product sequencing BD v3.1 - Help in removal of residues (reply: 1)
      
2323. Primers: advice? - (reply: 1)
      
2324. RNA and DNA extraction and PCR control - (reply: 1)
      
2325. correct bands in RT-PCR negative control - (reply: 14)
      
2326. About Q-PCR(real-time PCR) - COMPARE (reply: 1)
      
2327. fusion pcr - (reply: 1)
      
2328. dissociation curve of real time pcr products - 2 different peaks in dissociation curve (reply: 2)
      
2329. PCR product purification - (reply: 58)
      
2330. parse primer3 output - (reply: 3)
      
2331. primer3 - select primer in 3' end (reply: 3)
      
2332. bisulfite pcr primers - (reply: 1)
      
2333. bisulfite modification of dna for pcr - (reply: 1)
      
2334. The PCR Suite - a set of new primer design tools - (reply: 1)
      
2335. methylation specific PCR (MSP) - reproducibilty? (reply: 1)
      
2336. pcr ligation - problems with ligation (reply: 1)
      
2337. PCR - (reply: 1)
      
2338. PCR problem - can not get the target SSR band (reply: 1)
      
2339. RT-PCR, RNA isolation, etc. - (reply: 1)
      
2340. RT-PCR amplification problem - amplification problem (reply: 1)
      
2341. contamination PCR - (reply: 3)
      
2342. PCR problems - (reply: 4)
      
2343. Genomic PCR - (reply: 2)
      
2344. Is there anybody use Taq polymerase of Fermemtas - (reply: 20)
      
2345. multiplex PCR - (reply: 1)
      
2346. Amplification Problem - PCR Failure... (reply: 5)
      
2347. RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
      
2348. LacZ PCR - (reply: 1)
      
2349. about semi-quantity of PCR products on agarose gel - (reply: 2)
      
2350. Fusion PCR Protocol? - (reply: 6)
      
2351. PCR artefacts - (reply: 1)
      
2352. Primer dimers - (reply: 1)
      
2353. PCR Cloning - (reply: 1)
      
2354. RT-PCR 1 step - (reply: 2)
      
2355. does hot lid matter? - PCR problem (reply: 9)
      
2356. PCR and Primers - PCR-no products (reply: 4)
      
2357. Double strand cDNA sysnthesis - Using random primers (reply: 1)
      
2358. AMPLIFYING LONG FRAGMENTS - problems obtaining 3kb fragment (reply: 4)
      
2359. RNA contamination PCR - RNA contamination PCR (reply: 1)
      
2360. Amplification Failure of Unknown Reason - (reply: 1)
      
2361. APOE PCR conditions - RFLP e2, e3, e4? (reply: 5)
      
2362. Ammonium Sulfate PCR buffer - Ammonium Sulfate PCR buffer (reply: 1)
      
2363. degenerate primer - optimizing degenerate primers (reply: 1)
      
2364. Detection in SmartCycler RT-PCR - (reply: 1)
      
2365. Long PCR problem - Product smaller than expected size (reply: 3)
      
2366. Probes for Real-Time PCR - (reply: 3)
      
2367. gDNA contamination in RT-PCR - (reply: 2)
      
2368. DNA Contamination in RNA? - PCR of RNA (reply: 1)
      
2369. reference on PCR using degenerate primers - Help me find the reference (reply: 1)
      
2370. Help! DNA contamination in RNA and RT-PCR. - (reply: 1)
      
2371. simple pcr questions - simple pcr questions (reply: 1)
      
2372. Problems in my Multiplex PCR - (reply: 6)
      
2373. pcr select cdna subtraction - kit from clontech (reply: 1)
      
2374. PCR detection of water-bornebacteria - (reply: 1)
      
2375. Kit for Mycoplasma detection by PCR - (reply: 4)
      
2376. Sequencing PCR products - (reply: 2)
      
2377. Whole Cell PCR from BacteriaProtocols? - (reply: 4)
      
2378. cDNA PCR - Amplifying region using PCR from cDNA library (reply: 1)
      
2379. tomato DNA for PCR template - (reply: 1)
      
2380. tomato DNA for PCR - (reply: 1)
      
2381. Inverse /ligation-mediated PCR - 5'-flanking region amplification (reply: 1)
      
2382. Re: dNTP make up - (reply: 1)
      
2383. Need suggestions for degenerate primers - (reply: 2)
      
2384. My PCR is not work again - (reply: 6)
      
2385. phage PCR - (reply: 1)
      
2386. PCR from parraffin embeded samples - (reply: 2)
      
2387. Primer extension - (reply: 1)
      
2388. Primer design - Primer design for RT-PCR (reply: 1)
      
2389. I am afraid I got wrong Primer from company - (reply: 2)
      
2390. Difficult PCR amplification of 4kb genomic DNA - (reply: 3)
      
2391. long PCR for mtDNA - (reply: 1)
      
2392. Primer contamination - (reply: 2)
      
2393. PCR amplification in negative control - (reply: 1)
      
2394. PCR on cDNA libraries - (reply: 2)
      
2395. sequencing of Pcr product - (reply: 4)
      
2396. Blunting 3' ends with T4 polymerase - (reply: 1)
      
2397. Gene copy number by PCR - (reply: 1)
      
2398. Difference between RNase Protention and RT-PCR - (reply: 1)
      
2399. RT-PCR - (reply: 2)
      
2400. A variation of the usual PCRprotocol needed - (reply: 3)
      
2401. degenerated primer for nested RT PCR - (reply: 5)
      
2402. RNase and PCR - (reply: 1)
      
2403. RT-PCR - (reply: 1)
      
2404. pcr mutagenesis - (reply: 3)
      
2405. questions about degenerate primer PCR - (reply: 2)
      
2406. design a primer - how to determind a specific primer in a complete gonome? (reply: 1)
      
2407. RT-PCR - (reply: 1)
      
2408. dUTP used for PCR - (reply: 6)
      
2409. PCR and Cultures - PCR and Cultures (reply: 1)
      
2410. PCR with cell source - (reply: 1)
      
2411. PCR - (reply: 1)
      
2412. In situ PCR and hybridization in plant - In situ PCR/hybridization protocols (reply: 1)
      
2413. RT PCR - (reply: 1)
      
2414. Difference in RT PCR products when using total RNA or mRNA - (reply: 4)
      
2415. ligation problems - cloning a PCR fragment - into a double-cut vector (reply: 4)
      
2416. PCR mutagenesis - (reply: 1)
      
2417. RT-PCR, help needed - (reply: 2)
      
2418. M13 phage amplification,who canhelp? - (reply: 2)
      
2419. Overlap PCR that will notamplify - (reply: 1)
      
2420. Poor PCR result - (reply: 2)
      
2421. Error-prone PCR - (reply: 1)
      
2422. Rt-PCR help,come in - use RT (reply: 2)
      
2423. Dimers on SDS-PAGE - (reply: 2)
      
2424. PCR or Southern? - (reply: 3)
      
2425. Pcr cloning - how to get a single band (reply: 4)
      
2426. hot start Taq - Taq polymerase (reply: 1)
      
2427. RNA isolation from isolatedislets and RT-PCR - (reply: 1)
      
2428. How to find/check the highly conservative sequence - RT-PCR primers (reply: 2)
      
2429. How to PCR GC-rich inserts - (reply: 2)
      
2430. RT-PCR - (reply: 2)
      
2431. Nested PCR sensitivity - (reply: 3)
      
2432. pcr on genomic dna ( tailsmouse), HELP !!!! - (reply: 2)
      
2433. SOE PCR - (reply: 1)
      
2434. Bioinfomatics (ePCR) - (reply: 1)
      
2435. cloning of pcr product - (reply: 2)
      
2436. About Marker - for PCR (reply: 2)
      
2437. Genomic Boiling Prep for PCR - quick way to obtain PCR template (reply: 2)
      
2438. Trypsin - PCR amplification (reply: 1)
      
2439. making radioactive DNA probesusing PCR - (reply: 1)
      
2440. PCR problem - non specific bands - pcr problem (reply: 8)
      
2441. Scorpion Primers? - Synthese of Scorpion Primer (reply: 1)
      
2442. Poor PCR Results - (reply: 3)
      
2443. problem with Rt-PCR - (reply: 1)
      
2444. ABI377 Sequencing of PCR products - (reply: 1)
      
2445. amplifiable dna from bones - procedure for dna isolation from bones (reply: 4)
      
2446. How to clean agar contamination for colony PCR - (reply: 2)
      
2447. Cloning error prone PCR products - (reply: 1)
      
2448. PCR problems - (reply: 1)
      
2449. genotyping transgenic mice with PCR - (reply: 1)
      
2450. PCR restriction site - (reply: 2)
      
2451. deletion pcr - deletion pcr gene (reply: 3)
      
2452. Single-cell RT-PCR with only ~10-100 Cells - (reply: 2)
      
2453. Problems with PCR Purification - When I try to purify my PCR product I lose all of my DNA? (reply: 4)
      
2454. C-MYC AND CYCLIN D1  / RT-PCR - (reply: 2)
      
2455. Amount of genomic DNA for PCRamplication - (reply: 1)
      
2456. a question about RT-PCR - (reply: 1)
      
2457. Re: PCR cloning problem - (reply: 1)
      
2458. primer purification - search method for primer purifying (reply: 3)
      
2459. PCR amplification of the terminal repeat - (reply: 1)
      
2460. How to make PCR  using the gel solution - (reply: 1)
      
2461. primer design tools - primer designing (reply: 28)
      
2462. Software for MSP (methylation-specific PCR) primer design - (reply: 3)
      
2463. Bioinfomatics (ePCR) - (reply: 9)
      
2464. primer design - Deinococcus (reply: 1)
      

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