Abgene PCR master mix?-MgCl concentration - (Jul/28/2008 )
Hi,
does anybody know How to find the MgCl concentration of abgene PCR master mix?? do they publish it ?
I have seen in invitrogen they say it is 6mM. I get my ct values for this particular gene around 26 but I cannot amplify my third dilution (0.01).
http://lgmb.fmrp.usp.br/eg/protocolos/PSGQS.pdf
-pcrmossad-
-RatRace2000-