when to set up a RT-PCR for mRNA expression? - detect transcription of transgene (Aug/10/2008 )
Can any body let me know, when is the right time to confirm that the transgene is getting transcribed (set up RT-PCR experimet)?
Since the litters of F1 generation needs to be genotyped, I can not know which pups are transgenic before 3 weeks, does that mean the mouse needs to be sacrificed once it is transgene positive (after 3 weeks) and take out the tissue for RNA isolation?
Is it neccesary to do RT-PCR with all the transgenic pups (different litters from different mouse colonies?
Thanks for your time!
-starstar-
Where and When is your TG expressed?
If it exists in every tissue there's no need to sac the mouse, just take some blood.
-NYUlentivirus-