Sss1 treatment of PCR amplicons - Potential dumb question (Feb/01/2007 )

Hi there,

probably a dumb question , but nevertheless:

Can Sss1 treatment be used to methylate PCR amplicons? If yes, it would be possible to produce methylated and unmethylated controls of a certain target for BSP by simply amplifying a large region of the promoter with the fragment of interest for bisulfite treatment in it. The PCR amplicon should be completely free of methylation, unless treated with Sss1. Or does this only make sense, if you Sss1-treat genomic DNA?

Thanks in advance,

Krümel

absolutely,

if you perform direct BSP you can then mix the methylated and unmethylated amplicons and do direct sequencing after bisulfite treatment.

Nick