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Software available for Multiplex PCR primer designing - (Dec/02/2005 )

hai everyone

I am trying for the concurrent amplification of 5 different gene sequences, target is one but the position of gene is different. I want to check the compatibility of these primers for the Multiplex PCR assay. Is there any bioinformatic programme or software (freely available) for this purpose.

or, In other way is there any software available for specific Multiplex PCR primer designing.....?

thanking U.



Here is an online site that lets you do it for free. Hope it helps.


Primer3 works pretty good

you can also purchase licenses from eg. VisualOmp but it is quite expensive


Hi for multiplex analysis you should really use "fastPCR" as this has features specially designed for multiplex PCR analysis.