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Why random primers for RT-qPCR - (Oct/06/2005 )

Hi

Why is it better to use random primers and dTs for RT-PCR? If you use a random primer is it possible that the sequence of interest will not be coded?

-bosco-

QUOTE (bosco @ Oct 6 2005, 12:46 PM)
Hi

Why is it better to use random primers and dTs for RT-PCR? If you use a random primer is it possible that the sequence of interest will not be coded?



Forget it, I figured it out

-bosco-