PCR amplification of the terminal repeat - (Feb/28/2002 )
one of my PCR primer is located in the terminal repeat region and I have trouble in the amplification.
Anyone that have good ideas?
terminal repeat of what?
r u sayign u have repeats in ur constructs? or???
perhaps u can change the primers... that's one way. if u must use the repeats, u will always get heterogeneous band sizes. look for the right size.