condition for keeping integrity of RNA for RT-PCR - (Dec/12/2006 )
I'm new to doing RNA and I've been pretty nervous about keeping the integrity of the RNA for measuring RNA concentration and doing RT-PCR.
If i take out the RNA sample from -70 degrees and wait for it to thaw to the liquid phase so i can measure the RNA concentration and do RT-PCR, i'm still at risk of degrading the RNA, is that true???
Is there any way that i can minimize that chance?
Thank you so much for any inputs!
after extraction, i directly quantitae the RNA and aliquot it.
i use 1 aliquot per exp and thus the RNA is not degraded for an other exp. moreover concentrations are the same.