Is there anybody use Taq polymerase of Fermemtas - (Dec/08/2003 )
Is there anybody use Taq polymerase of Fermentas(MBI), or other products of this company, its Taq is so cheap that i'm afraid.....
works fine for most applications. havent tried it for anything bigger than 1.5 kb.
I have been using Fermentas´s products for a while and have no complaints whatsoever. Ive used different kinds of their polymerases (Taq, Pfu) and theyve worked flawlessly...tackling some products ive couldnt amplify with other brands. On the other hand, ive used many of their restriction enzymes and also worked great.
At first I was a little suspicious about the price, but it seems now that ive been overcharged by other companies for a long time...Fermentas´s enzymes are not expensive and work great, period.
Hope this helps....
Fermentas Taq has been used in my lab. No complaints about the taq, but they have a 6 week delay on getting it to us here in NZ. So, we're sticking to using Invitrogen who can deliver next day.
I have used it and it is not bad. In our lab, we prefer Replitaq or Amplitaq.
I'm used to perform PCR with the Fermentas Taq and I think it is as good as others' ,which are usually more expensive.
well my experience with fermentas taq has been bad, their enzymes are high fidelity ones and usually recommended for long range PCR.
for unknown reasons it never worked in our lab....we tried amplifying a 1kb product though!!
their enzymes are fine,just enjoy them.
I used extraction kit (for bacteria)of Fermentas. It's bad for me
---> low yield
---> don't have RNase
Their QC is sometimes fail. You will end up with multiple bands in your gels. at last, find out the Taq still bound will some DNA fragments during the manufactured. For normal PCR it is fine. But, strictly do not use for Real-time.