Insert PCR - (Jul/09/2007 )
Hi guys
What should I do after amplyfing my cDNA and running gel.
I picked up 96 clones for primary library and have my inserts.
What should I do next? I am completely confused?
I should grow them and then extract my vector and cDNA?
Maybe one could explain what I should do from where I am to sequencing step?
Greatful for help
Thanks
Robert
-Robertek-
QUOTE (Robertek @ Jul 9 2007, 12:34 PM)
Hi guys
What should I do after amplyfing my cDNA and running gel.
I picked up 96 clones for primary library and have my inserts.
What should I do next? I am completely confused?
I should grow them and then extract my vector and cDNA?
Maybe one could explain what I should do from where I am to sequencing step?
Greatful for help
Thanks
Robert
What should I do after amplyfing my cDNA and running gel.
I picked up 96 clones for primary library and have my inserts.
What should I do next? I am completely confused?
I should grow them and then extract my vector and cDNA?
Maybe one could explain what I should do from where I am to sequencing step?
Greatful for help
Thanks
Robert
Grow the 96 clones and make miniprep DNA and send them for sequencing.
With the results from sequencing, blast it and compare matches from the database.
-scolix-