PCR screening of colonies - (Jul/27/2007 )
how can i perform a positive control of such PCR screening? Suppose I am using E.coli JM109 for transformation
Hi, U can add some amount of positive vector to the untransformed strain and use as positive control.
Well, you will need a plasmid that contains the structure you are look for. That is in essence the possitive control.
But sometimes that is no possible. In situations where you are building a brand new plasmid, one that has never been made before, a possitive control is not possible. Regretfully the best that you can do is a negative control, have confidence in your ability and a prayer to the God of Molecular biology.
have confidence in your ability and a prayer to the God of Molecular biology.