designing a primer - (Jun/16/2007 )
Dear fellow friends,
Please help me how to design primer(s) for site-directed mutagenesis.
Any suggestion on how may I go about it...
hi,
this is from the user Dax,
There are some good primer design programs out there, but most of them are expensive. Though, I have successfully developed site-directed primers using PrimerX, an online program. It can be found at: http://bioinformatics.org/primerx/
and this is the program I use;
http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi
good luck
are you using a kit to achieve mutagenesis?
Thank you very much. Best regards.
this is from the user Dax,
There are some good primer design programs out there, but most of them are expensive. Though, I have successfully developed site-directed primers using PrimerX, an online program. It can be found at: http://bioinformatics.org/primerx/
and this is the program I use;
http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi
good luck
no i'm not using a kit
use a kit might be helpful.
go to some company's website (for eg. stratagene) which makes these kits. the site will have everything you need including programs to help design primers.