primer optimization - (May/22/2005 )
When i do the primer optimization should i use my sample or should i use other samples which i don't need with my target to find the optimal primer concentrations? If i use my sample for this optimization will not i waste my sample? How do you do?
Usually it depends on how difficult it is to get your template and how precious it is to you. If sample is limited I would try to optimise on something else that does have my gene of interest.