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Touch down real-time PCR? - (Jan/17/2006 )

hi all,

i was wondering if a touchdown pcr programm is suitable for qPCR?
might it increase the bias of the experiment ?

thanks for your thoughts and suggestions...



Why would you want to do that?

qPCR assumes that you've already optimized the primers and amplification temps. You want each amplification step to be the same over the forty cycles.