The best reverse transcriptase for RT PCR - (Sep/24/2007 )
Hye everyone. I have to buy reverse transcriptase for RT-PCR protocol. My supervisor suggested using AMV r.t. So, from your experience, which supplier provide the best rt? Thanks.
I use Invitrogen's M-MLV but they also have AMV, I guess. It works fine! You can also try Promega, NEB, Finnzymes or ABgene (been having very good experience with their DNA polymerases, cheap and good).
I prefer SuperScript III Reverse Transcriptase from invitrogen
I, also, prefer SSIII from Invitrogen.
Me too prefer Invitrogen's superscript III.
I've tried superscript II but it's been a rather disapointing experience! How better is SSIII?