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PCR of herbarium specimes - PCR of Caesalpinia herbarium specimes (Jun/21/2006 )

Hi every one,

I am trying to amplify some herbarium samples and i am having a hard time. I got very nice amplification using ITS or ETS, but I got almos nothing but a smirr using low-copy nuclear genes. I tried different PCR protocols, I didi Get rid of the junk before performing the PCR and nothing changed.
by the there any time machine available?
ok...I will be very happy with any suggestion.



here there's another post about herbarium samples and DNA extraction.

Maybe you won't find it that useful, but unfortunately I can't help you more then this... unsure.gif
Good luck!