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The same PCR gives different results?? - PCR (Aug/10/2005 )

I run PCR in three tubes in parallel, with exactly the same reagents added in a very short time range.

However, the three tubes gives different results. One gives a PCR product band (500bp) and a heavy larger band (1.6kbp), one gives larger band (1.6kbp) only, and one gives nothing.

How can the same reaction lead to different results?
Thanks for help!

-rabbitrabbit-

By the way, the template is a 4 kbp sized plasmid.

-rabbitrabbit-