The same PCR gives different results?? - PCR (Aug/10/2005 )
I run PCR in three tubes in parallel, with exactly the same reagents added in a very short time range.
However, the three tubes gives different results. One gives a PCR product band (500bp) and a heavy larger band (1.6kbp), one gives larger band (1.6kbp) only, and one gives nothing.
How can the same reaction lead to different results?
Thanks for help!
-rabbitrabbit-
By the way, the template is a 4 kbp sized plasmid.
-rabbitrabbit-