Real-Time PCR Amplicon Question - (Oct/30/2006 )
whats the amplicon size limit for real-time pcr? Is 300-400bp too big?
thanks
-BioDude1-
I've had amplicons that size work fine. I don't think you want to get much bigger than that however.
-tap14-
100-150bp is enough. I always use 100bp and it works well.
-fruit-