[PCR] Which substances cause inhinbition of Taq? - (Aug/23/2006 )
HI!
Which substances are known inhibitors of the Taq polymerase? (Paper?)
CU
Sven
www.google.com
sven, I would also like to add something...you often ask for papers with your questions
are you familiar with pubmed? if you go to pubmed, there is a very easy search function where you can likely get your questions answered more easily, and you already have the appropriate paper
D. Gelfand, 1989, PCR Technology, H. Erlich, ed. Stockton Press, 17-22.
D. Loffert, S. Stump, N. Schaffrath, M. Berkenkopf and J. Kang, 1997 PCR: effects of template quality. Qiagen News 1: 8-10.
Rebecca A. Reiss and Bridget Rutz, 1999. Quality control PCR: a method for detecting inhibitors of Taq DNA polymerase. Biotechniques 27 (5): 920-926.
Wittwer(1991) BioTechniques 10.1, 82
sure
OK:
I always ask for papers, because if someone asks me, i normally just give him the values i know, although i know the paper. But if you want to write something it is in my opinion not enough to say "i heard that,..." - that is just a hint, but NO information.
The point is: i think there are many people who know several papers, dealing with certain problems. Finding papers via PubMed (,...) is sometimes difficult (i tried to find out something about gel characteristics - there are values in wikipedia, but there ist no proof mentioned), and after half an hour of searching i think its OK to ask other people, who maybe know the answer. Furthermore other people have the chance to read the topic, and that makes their search easier.
I agree with you that "posting without previous searching, and let other people doing the work" is not the function of a Forum, but "sharing information" is.
My special problem is, that i do not have access to many papers, and that makes inquiry difficult (for a certain primer i needed to days to find out who developed it because i had no access to papers that mentioned it)....
well, yeah, but most of us don't necessarily have links to papers citing inhibitors of Taq hanging out around our desktops...I think a quick few minutes on NEB's website will give you the information plus a reference...I also think a quick few minutes at Promega, Stratagene, ABI, any of the PCR-supplier websites would give you the same information with references in the technical format you are requesting
I'm not trying to pick on you sven, it really does seem to me that if you want a comprehensive list with a reference, you're much more likely to get it quickly if you do it yourself. this is just my opinion and I'm not trying to anger you or anything, but the best way to do a literature search (which seems to be what you want) is to use something like Pubmed, even if it takes longer than 30 minutes. I've spent hours on Pubmed 