multiple displacement amplification - (May/14/2007 )
Hi! I am trying to find more infos on this tecniques that i will have to use for whole genomic amplification from few cells eg how does the dna polymerase phi29 displaces the newly synthesized fagments? and how are the hexanucleotide primers designed? how much risk there is of allele drop out when aplifying dna fragments after MDA?
Is there any free pcr manual on line I can consult about it?
I found several articles about it......but would like to understand better the basics.
have you tried www.biotechniques.com ?? you might find something useful there. good luck on your research.
I fund I nice article there: THANX! :-)!