RT-PCR for small RNAs - (Jan/07/2009 )
Hi all, I'd like to express snoRNAs (about 90nt) in mammalian cells and want to detect its expression by RT-PCR. I'm not sure can ordinary RT-PCR work for this purpose. Should I use random hexamer or gene-specific primer? I also plan to do northern blot in the future. Do I need to run acrylamide/urea gel or ordinary denatured agrose gel? Thanks so much!
See if you find answers in many small RNA Protocols here.